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Journal of Cell Science 114, 3857-3863 (2001)
© 2001 The Company of Biologists Limited

RESEARCH ARTICLE

The abundant retinal protein of the Chlamydomonas eye is not the photoreceptor for phototaxis and photophobic responses

Markus Fuhrmann{ddagger}, Alke Stahlberg, Elena Govorunova*, Simone Rank and Peter Hegemann

Institut für Biochemie I, Universität Regensburg, 93040 Regensburg, Germany
* Present address: Moscow state university, Biology Department, 119899 Moscow, Russia

{ddagger}Author for correspondence (e-mail: markus.fuhrmann{at}vkl.uni-regensburg.de)

Accepted July 16, 2001

The chlamyopsin gene (cop) encodes the most abundant eyespot protein in the unicellular green alga Chlamydomonas reinhardtii. This opsin-related protein (COP) binds retinal and was thought to be the photoreceptor controlling photomovement responses via a set of photoreceptor currents. Unfortunately, opsin-deficient mutants are not available and targeted disruption of non-selectable nuclear genes is not yet possible in any green alga. Here we show that intron-containing gene fragments directly linked to their intron-less antisense counterpart provide efficient post-transcriptional gene silencing (PTGS) in C. reinhardtii, thus allowing an efficient reduction of a specific gene product in a green alga. In opsin-deprived transformants, flash-induced photoreceptor currents (PC) are left unchanged. Moreover, photophobic responses as studied by motion analysis and phototaxis tested in a light-scattering assay were indistinguishable from the responses of untransformed wild-type cells. We conclude that phototaxis and photophobic responses in C. reinhardtii are triggered by an as yet unidentified rhodopsin species.

Key words: RNAi, Chlamyopsin, Antisense




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© The Company of Biologists Ltd 2001