|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
RESEARCH ARTICLE |
2ß1-integrin expression
1 Prostate Research Group, Department of Surgery, The Medical School, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK
2 Prostate Research Group, Department of Oncology, Western General Hospital, University of Edinburgh, Edinburgh, EH4 2XU, UK
3 YCR Cancer Research Unit, Department of Biology, University of York, PO Box 373, York, YO1 5YN, UK
*Author for correspondence (e-mail: anne.collins{at}newcastle.ac.uk)
Accepted July 23, 2001
A major impediment to our understanding of the biology of stem cells is the inability to distinguish them from their differentiating progeny. We made use of the known association of stem cells with basement membranes to isolate prostate epithelial stem cells. We show that, in vivo, putative stem cells express higher levels of the 
2-integrin subunit than other cells within the basal layer. Approximately 1% of basal cells examined by confocal microscopy were integrin ‘bright’, and these cells can be selected directly from the tissue on the basis of rapid adhesion to type I collagen. This selected population has a basal phenotype, as determined by expression of CK5 and CK14 and lack of expression of the differentiation-specific markers prostate specific antigen (PSA) and prostatic acid phosphatase (PAP), and has a fourfold greater ability to form colonies in vitro than the total basal population. These putative stem cells are distinguished from other basal cells by their ability to generate prostate-like glands in vivo with morphologic and immuno-histochemical evidence of prostate-specific differentiation. These properties are consistent with a stem cell origin. Furthermore, the presence of surface integrins on prostate stem cells suggests that these cells share common pathways with stem cells in other tissues.
Key words: Stem cells, Integrins, Prostate
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Rutella, G. Bonanno, A. Procoli, A. Mariotti, M. Corallo, M. G. Prisco, A. Eramo, C. Napoletano, D. Gallo, A. Perillo, et al. Cells with Characteristics of Cancer Stem/Progenitor Cells Express the CD133 Antigen in Human Endometrial Tumors Clin. Cancer Res., July 1, 2009; 15(13): 4299 - 4311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.M. Shen, X. Wang, K.D. Economides, D. Walker, and C. Abate-Shen Progenitor Cells for the Prostate Epithelium: Roles in Development, Regeneration, and Cancer Cold Spring Harb Symp Quant Biol, January 15, 2009; (2009) sqb.2008.73.050v1. [Abstract] [PDF]
|
|
|
|
 |
|
 A. S. Goldstein, D. A. Lawson, D. Cheng, W. Sun, I. P. Garraway, and O. N. Witte Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics PNAS, December 30, 2008; 105(52): 20882 - 20887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Vander Griend, W. L. Karthaus, S. Dalrymple, A. Meeker, A. M. DeMarzo, and J. T. Isaacs The Role of CD133 in Normal Human Prostate Stem Cells and Malignant Cancer-Initiating Cells Cancer Res., December 1, 2008; 68(23): 9703 - 9711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.U. Lukacs, D.A. Lawson, L. Xin, Y. Zong, I. Garraway, A.S. Goldstein, S. Memarzadeh, and O.N. Witte Epithelial Stem Cells of the Prostate and Their Role in Cancer Progression Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.012v1. [Abstract] [PDF]
|
|
|
|
 |
|
 B. Bhatia, M. Jiang, M. Suraneni, L. Patrawala, M. Badeaux, R. Schneider-Broussard, A. S. Multani, C. R. Jeter, T. Calhoun-Davis, L. Hu, et al. Critical and Distinct Roles of p16 and Telomerase in Regulating the Proliferative Life Span of Normal Human Prostate Epithelial Progenitor Cells J. Biol. Chem., October 10, 2008; 283(41): 27957 - 27972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Kirkland and H. Ying {alpha}2{beta}1 Integrin Regulates Lineage Commitment in Multipotent Human Colorectal Cancer Cells J. Biol. Chem., October 10, 2008; 283(41): 27612 - 27619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. P. Risbridger and R. A. Taylor Regulation of Prostatic Stem Cells by Stromal Niche in Health and Disease Endocrinology, September 1, 2008; 149(9): 4303 - 4306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Konishi, K. Shimada, M. Nakamura, E. Ishida, I. Ota, N. Tanaka, and K. Fujimoto Function of JunB in Transient Amplifying Cell Senescence and Progression of Human Prostate Cancer Clin. Cancer Res., July 15, 2008; 14(14): 4408 - 4416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Maitland and A. T. Collins Prostate Cancer Stem Cells: A New Target for Therapy J. Clin. Oncol., June 10, 2008; 26(17): 2862 - 2870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mimeault, P. P. Mehta, R. Hauke, and S. K. Batra Functions of Normal and Malignant Prostatic Stem/Progenitor Cells in Tissue Regeneration and Cancer Progression and Novel Targeting Therapies Endocr. Rev., April 1, 2008; 29(2): 234 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Patrawala, T. Calhoun-Davis, R. Schneider-Broussard, and D. G. Tang Hierarchical Organization of Prostate Cancer Cells in Xenograft Tumors: The CD44+{alpha}2{beta}1+ Cell Population Is Enriched in Tumor-Initiating Cells Cancer Res., July 15, 2007; 67(14): 6796 - 6805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gu, J. Yuan, M. Wills, and S. Kasper Prostate Cancer Cells with Stem Cell Characteristics Reconstitute the Original Human Tumor In vivo Cancer Res., May 15, 2007; 67(10): 4807 - 4815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Miki, B. Furusato, H. Li, Y. Gu, H. Takahashi, S. Egawa, I. A. Sesterhenn, D. G. McLeod, S. Srivastava, and J. S. Rhim Identification of Putative Stem Cell Markers, CD133 and CXCR4, in hTERT-Immortalized Primary Nonmalignant and Malignant Tumor-Derived Human Prostate Epithelial Cell Lines and in Prostate Cancer Specimens Cancer Res., April 1, 2007; 67(7): 3153 - 3161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, N. C. McKnight, T. Zhang, M. L. Lu, S. P. Balk, and X. Yuan SOX9 Is Expressed in Normal Prostate Basal Cells and Regulates Androgen Receptor Expression in Prostate Cancer Cells Cancer Res., January 15, 2007; 67(2): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Lawson, L. Xin, R. U. Lukacs, D. Cheng, and O. N. Witte Isolation and functional characterization of murine prostate stem cells PNAS, January 2, 2007; 104(1): 181 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. V. Litvinov, D. J. Vander Griend, Y. Xu, L. Antony, S. L. Dalrymple, and J. T. Isaacs Low-Calcium Serum-Free Defined Medium Selects for Growth of Normal Prostatic Epithelial Stem Cells. Cancer Res., September 1, 2006; 66(17): 8598 - 8607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Hall, J. Dai, K. L. van Golen, E. T. Keller, and M. W. Long Type I Collagen Receptor ({alpha}2{beta}1) Signaling Promotes the Growth of Human Prostate Cancer Cells within the Bone. Cancer Res., September 1, 2006; 66(17): 8648 - 8654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Heer, A. T. Collins, C. N. Robson, B. K. Shenton, and H. Y. Leung KGF suppresses {alpha}2{beta}1 integrin function and promotes differentiation of the transient amplifying population in human prostatic epithelium. J. Cell Sci., April 1, 2006; 119(Pt 7): 1416 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanatsu-Shinohara, K. Inoue, J. Lee, H. Miki, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara Anchorage-Independent Growth of Mouse Male Germline Stem Cells In Vitro Biol Reprod, March 1, 2006; 74(3): 522 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Campos, L. Decker, V. Taylor, and W. Skarnes Notch, Epidermal Growth Factor Receptor, and beta1-Integrin Pathways Are Coordinated in Neural Stem Cells J. Biol. Chem., February 24, 2006; 281(8): 5300 - 5309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Collins, P. A. Berry, C. Hyde, M. J. Stower, and N. J. Maitland Prospective Identification of Tumorigenic Prostate Cancer Stem Cells Cancer Res., December 1, 2005; 65(23): 10946 - 10951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Leone, J. B. Relvas, L. S. Campos, S. Hemmi, C. Brakebusch, R. Fassler, C. ffrench-Constant, and U. Suter Regulation of neural progenitor proliferation and survival by {beta}1 integrins J. Cell Sci., June 15, 2005; 118(12): 2589 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. Burger, X. Xiong, S. Coetzee, S. N. Salm, D. Moscatelli, K. Goto, and E. L. Wilson Sca-1 expression identifies stem cells in the proximal region of prostatic ducts with high capacity to reconstitute prostatic tissue PNAS, May 17, 2005; 102(20): 7180 - 7185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D M Peehl Primary cell cultures as models of prostate cancer development Endocr. Relat. Cancer, March 1, 2005; 12(1): 19 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.A. LAWSON, L. XIN, R. LUKACS, Q. XU, D. CHENG, and O.N. WITTE Prostate Stem Cells and Prostate Cancer Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 187 - 196. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Kurita, R. T. Medina, A. A. Mills, and G. R. Cunha Role of p63 and basal cells in the prostate Development, October 15, 2004; 131(20): 4955 - 4964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Alam, M. J. O'Hare, I. Laczko, A. Freeman, F. Al-Beidh, J. R. Masters, and D. L. Hudson Differential Expression of CD44 During Human Prostate Epithelial Cell Differentiation J. Histochem. Cytochem., August 1, 2004; 52(8): 1083 - 1090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Richardson, C. N. Robson, S. H. Lang, D. E. Neal, N. J. Maitland, and A. T. Collins CD133, a novel marker for human prostatic epithelial stem cells J. Cell Sci., July 15, 2004; 117(16): 3539 - 3545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-D. Wang, J. Shou, P. Wong, D. M. French, and W.-Q. Gao Notch1-expressing Cells Are Indispensable for Prostatic Branching Morphogenesis during Development and Re-growth Following Castration and Androgen Replacement J. Biol. Chem., June 4, 2004; 279(23): 24733 - 24744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Keay, F. Seillier-Moiseiwitsch, C.-O. Zhang, T. C. Chai, and J. Zhang Changes in human bladder epithelial cell gene expression associated with interstitial cystitis or antiproliferative factor treatment Physiol Genomics, July 7, 2003; 14(2): 107 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. V. Litvinov, A. M. De Marzo, and J. T. Isaacs Is the Achilles' Heel for Prostate Cancer Therapy a Gain of Function in Androgen Receptor Signaling? J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 2972 - 2982. [Full Text] [PDF]
|
|
