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Journal of Cell Science 114, 4001-4011 (2001)
© 2001 The Company of Biologists Limited

RESEARCH ARTICLE

Distinct changes in intranuclear lamin A/C organization during myoblast differentiation

Bh. Muralikrishna, Jyotsna Dhawan, Nandini Rangaraj and Veena K. Parnaik*

Centre for Cellular and Molecular Biology, Hyderabad 500 007, India

*Address for correspondence (e-mail: veenap{at}ccmb.ap.nic.in)

Accepted August 3, 2001

Intranuclear lamin foci or speckles have been observed in various cell types. In order to explore the possibility of changes in internal lamin organization during muscle differentiation, we have examined the appearance of A-type lamin speckles that associate with RNA splicing factor speckles in C2C12 myoblasts and myotubes. Lamin speckles were observed in dividing myoblasts but disappeared early during the course of differentiation in postmitotic myocytes, and were absent in myotubes and muscle fibers. However, no changes were seen in the typical peripheral organization of lamins A/C or B1 or in RNA splicing factor speckles. Lamin speckles were also absent in quiescent myoblasts but reappeared as cells were reactivated to enter the cell cycle. These changes were not observed in other quiescent cell types. Immunoblot analysis indicated that the abundance and migration of lamins A and C was not altered in differentiated myoblasts. When myotube or quiescent myoblast nuclei were extracted with nucleases and detergent, a uniformly stained internal lamina was revealed, indicating that lamins A/C were antigenically masked in these cells, probably owing to structural reorganization of the lamina during differentiation or quiescence. Our results suggest that muscle cell differentiation is accompanied by regulated rearrangements in the organization of the A-type lamins.

Key words: Nuclear lamina, Lamin A, Muscle differentiation, Myoblasts


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© The Company of Biologists Ltd 2001