spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Touitou, I.
Right arrow Articles by Pruliere, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Touitou, I.
Right arrow Articles by Pruliere, G.

Journal of Cell Science, Vol 114, Issue 3 481-491, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Boursin, a sea urchin bimC kinesin protein, plays a role in anaphase and cytokinesis

I Touitou, G Lhomond and G Pruliere
UMR 7009 CNRS-UPMC, Observatoire Oceanologique, 06234 Villefranche-Sue-Mer Cedex, France. touitou@obs-vlfr.fr

We have isolated and characterized Boursin, a kinesin-related protein of the bimC family, from Paracentrotus lividus sea urchin eggs. Boursin is expressed at high levels in eggs and embryos during early cleavage stages. Boursin was found to be associated with different parts of the mitotic spindle from early prophase to telophase. Expression of a form of the protein predicted to act as a dominant negative mutant caused severe defects in cell division and resulted in the formation of embryos with polyploid and multiastral blastomeres. Immunofluorescence analysis indicated that these defects did not arise from failure in either centrosome separation or bipolar spindle formation. Time-lapse observations showed rather that these perturbations in cell division resulted from abnormal anaphase and failure to complete cytokinesis. These phenotypes differ from the phenotype described following perturbation of the function of bimC family members in other organisms. Our study has thus uncovered roles for a bimC kinesin in late stages of cell division.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
N. Movshovich, V. Fridman, A. Gerson-Gurwitz, I. Shumacher, I. Gertsberg, A. Fich, M. A. Hoyt, B. Katz, and L. Gheber
Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells
J. Cell Sci., August 1, 2008; 121(15): 2529 - 2539.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. R. Hildebrandt, L. Gheber, T. Kingsbury, and M. A. Hoyt
Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function
J. Biol. Chem., September 8, 2006; 281(36): 26004 - 26013.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. D. Bishop, Z. Han, and J. M. Schumacher
The Caenorhabditis elegans Aurora B Kinase AIR-2 Phosphorylates and Is Required for the Localization of a BimC Kinesin to Meiotic and Mitotic Spindles
Mol. Biol. Cell, February 1, 2005; 16(2): 742 - 756.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001