|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 4 653-661, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
SG Zeitlin, CM Barber, CD Allis and K Sullivan
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA.
After DNA replication, cells condense their chromosomes in order to segregate them during mitosis. The condensation process as well as subsequent segregation requires phosphorylation of histone H3 at serine 10. Histone H3 phosphorylation initiates during G2 in pericentric foci prior to H3 phosphorylation in the chromosome arms. Centromere protein A (CENP-A), a histone H3-like protein found uniquely at centromeres, contains a sequence motif similar to that around H3 Ser10, suggesting that CENP-A phosphorylation might be linked to pericentric initiation of histone H3 phosphorylation. To test this hypothesis, we generated peptide antibodies against the putative phosphorylation site of CENP-A. ELISA, western blot and immunocytochemical analyses show that CENP-A is phosphorylated at the shared motif. Simultaneous co-detection demonstrates that phosphorylation of CENP-A and histone H3 are separate events in G2/M. CENP-A phosphorylation occurs after both pericentric initiation and genome-wide stages of histone H3 phosphorylation. Quantitative immunocytochemistry reveals that CENP-A phosphorylation begins in prophase and reaches maximal levels in prometaphase. CENP-A phosphoepitope reactivity is lost during anaphase and becomes undetectable in telophase cells. Duplication of prekinetochores, detected as the doubling of CENP-A foci, occurs prior to complete histone H3 phosphorylation in G2. Mitotic phosphorylation of histone H3-family proteins shows tight spatial and temporal control, occurring in three phases: (1) pericentric H3 phosphorylation, (2) chromosome arm H3 phosphorylation and (3) CENP-A phosphorylation at kinetochores. These observations reveal new cytological landmarks characteristic of G2 progression.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Ferri, H. Bouzinba-Segard, G. Velasco, F. Hube, and C. Francastel Non-coding murine centromeric transcripts associate with and potentiate Aurora B kinase Nucleic Acids Res., June 19, 2009; (2009) gkp529v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Faragher, X.-M. Sun, M. Butterworth, N. Harper, M. Mulheran, S. Ruchaud, W. C. Earnshaw, and G. M. Cohen Death Receptor-induced Apoptosis Reveals a Novel Interplay between the Chromosomal Passenger Complex and CENP-C during Interphase Mol. Biol. Cell, April 1, 2007; 18(4): 1337 - 1347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schrofelbauer, Q. Yu, S. G. Zeitlin, and N. R. Landau Human Immunodeficiency Virus Type 1 Vpr Induces the Degradation of the UNG and SMUG Uracil-DNA Glycosylases J. Virol., September 1, 2005; 79(17): 10978 - 10987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Hake, B. A. Garcia, M. Kauer, S. P. Baker, J. Shabanowitz, D. F. Hunt, and C. D. Allis Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes PNAS, May 3, 2005; 102(18): 6344 - 6349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Kamakaka and S. Biggins Histone variants: deviants? Genes & Dev., February 1, 2005; 19(3): 295 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, X. Li, J. B. Marshall, C. X. Zhong, and R. K. Dawe Phosphoserines on Maize CENTROMERIC HISTONE H3 and Histone H3 Demarcate the Centromere and Pericentromere during Chromosome Segregation PLANT CELL, February 1, 2005; 17(2): 572 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Ishov, O. V. Vladimirova, and G. G. Maul Heterochromatin and ND10 are cell-cycle regulated and phosphorylation-dependent alternate nuclear sites of the transcription repressor Daxx and SWI/SNF protein ATRX J. Cell Sci., August 1, 2004; 117(17): 3807 - 3820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Shibata and M. Murata Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana J. Cell Sci., June 15, 2004; 117(14): 2963 - 2970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Crosio, E. Heitz, C. D. Allis, E. Borrelli, and P. Sassone-Corsi Chromatin remodeling and neuronal response: multiple signaling pathways induce specific histone H3 modifications and early gene expression in hippocampal neurons J. Cell Sci., December 15, 2003; 116(24): 4905 - 4914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kawajiri, Y. Yasui, H. Goto, M. Tatsuka, M. Takahashi, K.-i. Nagata, and M. Inagaki Functional Significance of the Specific Sites Phosphorylated in Desmin at Cleavage Furrow: Aurora-B May Phosphorylate and Regulate Type III Intermediate Filaments during Cytokinesis Coordinatedly with Rho-kinase Mol. Biol. Cell, April 1, 2003; 14(4): 1489 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Preuss, G. Landsberg, and K. H. Scheidtmann Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase Nucleic Acids Res., February 1, 2003; 31(3): 878 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Jenuwein and C. D. Allis Translating the Histone Code Science, August 10, 2001; 293(5532): 1074 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Zeitlin, R. D. Shelby, and K. F. Sullivan CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis J. Cell Biol., December 24, 2001; 155(7): 1147 - 1158. [Abstract] [Full Text] [PDF]
|
|
