|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 4 719-726, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
MI Milewski, JE Mickle, JK Forrest, DM Stafford, BD Moyer, J Cheng, WB Guggino, BA Stanton and GR Cutting
Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21278, USA.
Localization of ion channels and transporters to the correct membrane of polarized epithelia is important for vectorial ion movement. Prior studies have shown that the cytoplasmic carboxyl terminus of the cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the apical localization of this protein. Here we show that the C-terminal tail alone, or when fused to the green fluorescent protein (GFP), can localize to the apical plasma membrane, despite the absence of transmembrane domains. Co-expression of the C terminus with full-length CFTR results in redistribution of CFTR from apical to basolateral membranes, indicating that both proteins interact with the same target at the apical membrane. Amino acid substitution and deletion analysis confirms the importance of a PDZ-binding motif D-T-R-L> for apical localization. However, two other C-terminal regions, encompassing amino acids 1370-1394 and 1404-1425 of human CFTR, are also required for localizing to the apical plasma membrane. Based on these results, we propose a model of polarized distribution of CFTR, which includes a mechanism of selective retention of this protein in the apical plasma membrane and stresses the requirement for other C-terminal sequences in addition to a PDZ-binding motif.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Stenzel, C. P. Fetzer, R. Heumann, and K. S. Erdmann PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells J. Cell Sci., September 15, 2009; 122(18): 3374 - 3384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Broere, J. Hillesheim, B. Tuo, H. Jorna, A. B. Houtsmuller, S. Shenolikar, E. J. Weinman, M. Donowitz, U. Seidler, H. R. de Jonge, et al. Cystic Fibrosis Transmembrane Conductance Regulator Activation Is Reduced in the Small Intestine of Na+/H+ Exchanger 3 Regulatory Factor 1 (NHERF-1)- but Not NHERF-2-deficient Mice J. Biol. Chem., December 28, 2007; 282(52): 37575 - 37584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bossard, A. Robay, G. Toumaniantz, S. Dahimene, F. Becq, J. Merot, and C. Gauthier NHE-RF1 protein rescues {Delta}F508-CFTR function Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1085 - L1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wolde, A. Fellows, J. Cheng, A. Kivenson, B. Coutermarsh, L. Talebian, K. Karlson, A. Piserchio, D. F. Mierke, B. A. Stanton, et al. Targeting CAL as a Negative Regulator of {Delta}F508-CFTR Cell-Surface Expression: AN RNA INTERFERENCE AND STRUCTURE-BASED MUTAGENETIC APPROACH J. Biol. Chem., March 16, 2007; 282(11): 8099 - 8109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mazzochi, D. J. Benos, and P. R. Smith Interaction of epithelial ion channels with the actin-based cytoskeleton Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1113 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Mason, K. E. Allen, and C. W. Slayman Effects of C-terminal Truncations on Trafficking of the Yeast Plasma Membrane H+-ATPase J. Biol. Chem., August 18, 2006; 281(33): 23887 - 23898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sharma, S. H. Low, S. Misra, B. Pallavi, and T. Weimbs Apical targeting of syntaxin 3 is essential for epithelial cell polarity J. Cell Biol., June 19, 2006; 173(6): 937 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guerra, T. Fanelli, M. Favia, S. M. Riccardi, G. Busco, R. A. Cardone, S. Carrabino, E. J. Weinman, S. J. Reshkin, M. Conese, et al. Na+/H+ Exchanger Regulatory Factor Isoform 1 Overexpression Modulates Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Activity in Human Airway 16HBE14o- Cells and Rescues {Delta}F508 CFTR Functional Expression in Cystic Fibrosis Cells J. Biol. Chem., December 9, 2005; 280(49): 40925 - 40933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-Q. Sun, N. Balasubramaniyan, C.-J. Liu, M. Shahid, and F. J. Suchy Association of the 16-kDa Subunit c of Vacuolar Proton Pump with the Ileal Na+-dependent Bile Acid Transporter: PROTEIN-PROTEIN INTERACTION AND INTRACELLULAR TRAFFICKING J. Biol. Chem., April 16, 2004; 279(16): 16295 - 16300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Haggie, B. A. Stanton, and A. S. Verkman Increased Diffusional Mobility of CFTR at the Plasma Membrane after Deletion of Its C-terminal PDZ Binding Motif J. Biol. Chem., February 13, 2004; 279(7): 5494 - 5500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Inukai, A. M. Shewan, W. S. Pascoe, S. Katayama, D. E. James, and Y. Oka Carboxy Terminus of Glucose Transporter 3 Contains an Apical Membrane Targeting Domain Mol. Endocrinol., February 1, 2004; 18(2): 339 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Guggino The Cystic Fibrosis Transmembrane Regulator Forms Macromolecular Complexes with PDZ Domain Scaffold Proteins Proceedings of the ATS, January 1, 2004; 1(1): 28 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Konno, T. Ebihara, K. Hisaeda, T. Uchiumi, T. Nakamura, T. Shirakusa, M. Kuwano, and M. Wada Identification of Domains Participating in the Substrate Specificity and Subcellular Localization of the Multidrug Resistance Proteins MRP1 and MRP2 J. Biol. Chem., June 13, 2003; 278(25): 22908 - 22917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Benharouga, M. Sharma, J. So, M. Haardt, L. Drzymala, M. Popov, B. Schwapach, S. Grinstein, K. Du, and G. L. Lukacs The Role of the C Terminus and Na+/H+ Exchanger Regulatory Factor in the Functional Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Nonpolarized Cells and Epithelia J. Biol. Chem., June 6, 2003; 278(24): 22079 - 22089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Caplan How megalin finds its way: identification of a novel apical sorting motif. Focus on "Identification of an apical sorting determinant in the cytoplasmic tail of megalin" Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1101 - C1104. [Full Text] [PDF]
|
|
|
|
|
|
H. Lohi, G. Lamprecht, D. Markovich, A. Heil, M. Kujala, U. Seidler, and J. Kere Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domains Am J Physiol Cell Physiol, March 1, 2003; 284(3): C769 - C779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Ostedgaard, C. Randak, T. Rokhlina, P. Karp, D. Vermeer, K. J. Ashbourne Excoffon, and M. J. Welsh Effects of C-terminal deletions on cystic fibrosis transmembrane conductance regulator function in cystic fibrosis airway epithelia PNAS, February 18, 2003; 100(4): 1937 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Swiatecka-Urban, M. Duhaime, B. Coutermarsh, K. H. Karlson, J. Collawn, M. Milewski, G. R. Cutting, W. B. Guggino, G. Langford, and B. A. Stanton PDZ Domain Interaction Controls the Endocytic Recycling of the Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., October 11, 2002; 277(42): 40099 - 40105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Milewski, J. E. Mickle, J. K. Forrest, B. A. Stanton, and G. R. Cutting Aggregation of Misfolded Proteins Can Be a Selective Process Dependent upon Peptide Composition J. Biol. Chem., September 6, 2002; 277(37): 34462 - 34470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. M. Fernandez, Z. Hollo, A. Kern, E. Bakos, P. A. Fischer, P. Borst, and R. Evers Role of the N-terminal Transmembrane Region of the Multidrug Resistance Protein MRP2 in Routing to the Apical Membrane in MDCKII Cells J. Biol. Chem., August 16, 2002; 277(34): 31048 - 31055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zheng and J. E. Sadler Mucin-like Domain of Enteropeptidase Directs Apical Targeting in Madin-Darby Canine Kidney Cells J. Biol. Chem., February 22, 2002; 277(9): 6858 - 6863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Chen, C. Cutler, C. Jacques, E. Denamur, G. Lecointre, B. Mercier, G. Cramb, and C. Ferec A Combined Analysis of the Cystic Fibrosis Transmembrane Conductance Regulator: Implications for Structure and Disease Models Mol. Biol. Evol., September 1, 2001; 18(9): 1771 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Karthikeyan, T. Leung, and J. A. A. Ladias Structural Basis of the Na+/H+ Exchanger Regulatory Factor PDZ1 Interaction with the Carboxyl-terminal Region of the Cystic Fibrosis Transmembrane Conductance Regulator J. Biol. Chem., June 1, 2001; 276(23): 19683 - 19686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. Li, S. W. Straight, and D. B. Kershaw PDZ domain-mediated interaction of rabbit podocalyxin and Na+/H+ exchange regulatory factor-2 Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1129 - F1139. [Abstract] [Full Text] [PDF]
|
|
