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Journal of Cell Science, Vol 114, Issue 4 747-750, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase

J Malinsky, K Koberna, D Stanek, M Masata, I Votruba and I Raska
Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Albertov 4, 128 00 Prague 2, Czech Republic.

Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2'-deoxythymidine analogues 5-chloro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2'-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase.


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 Mol. Biol. CellHome page
C. Conti, B. Sacca, J. Herrick, C. Lalou, Y. Pommier, and A. Bensimon
Replication Fork Velocities at Adjacent Replication Origins Are Coordinately Modified during DNA Replication in Human Cells
Mol. Biol. Cell, August 1, 2007; 18(8): 3059 - 3067.
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© The Company of Biologists Ltd 2001