|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 5 931-939, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
P Murray and D Edgar
Department of Human Anatomy and Cell Biology, The University of Liverpool, Ashton Street, Liverpool L69 3GE, UK.
Both the extracellular matrix and parathyroid hormone-related peptide (PTHrP) have been implicated in the differentiation and migration of extra-embryonic endodermal cells in the pre-implantation mammalian blastocyst. In order to define the individual roles and interactions between these factors in endodermal differentiation, we have used embryoid bodies derived from Lamc1(-/-) embryonic stem cells that lack basement membranes. The results show that in the absence of basement membranes, increased numbers of both visceral and parietal endodermal cells differentiate, but they fail to form organised epithelia. Furthermore, although parietal endodermal cells only migrate away from control embryoid bodies in the presence of PTHrP, they readily migrate from Lamc1(-/-) embryoid bodies in the absence of PTHrP, and this migration is unaffected by PTHrP. Thus, the basement membrane between epiblast and extra-embryonic endoderm is required for the proper organisation of visceral and parietal endodermal cells and also restricts their differentiation to maintain the population of primitive endodermal stem cells. Moreover, this basement membrane inhibits migration of parietal endodermal cells, the role of PTHrP being to stimulate delamination of parietal endodermal cells from the basement membrane rather than promoting migration per se.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Egea, C. Erlacher, E. Montanez, I. Burtscher, S. Yamagishi, M. Hess, F. Hampel, R. Sanchez, M. T. Rodriguez-Manzaneque, M. R. Bosl, et al. Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesoderm differentiation Genes & Dev., December 1, 2008; 22(23): 3349 - 3362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Futaki, Y. Hayashi, T. Emoto, C. N. Weber, and K. Sekiguchi Sox7 Plays Crucial Roles in Parietal Endoderm Differentiation in F9 Embryonal Carcinoma Cells through Regulating Gata-4 and Gata-6 Expression Mol. Cell. Biol., December 1, 2004; 24(23): 10492 - 10503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Takito and Q. Al-Awqati Conversion of ES cells to columnar epithelia by hensin and to squamous epithelia by laminin J. Cell Biol., September 27, 2004; 166(7): 1093 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Miner, C. Li, J. L. Mudd, G. Go, and A. E. Sutherland Compositional and structural requirements for laminin and basement membranes during mouse embryo implantation and gastrulation Development, May 15, 2004; 131(10): 2247 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gao, H. Y. Shi, C. Daughty, N. Cella, and M. Zhang Maspin plays an essential role in early embryonic development Development, April 1, 2004; 131(7): 1479 - 1489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sasaki, R. Fassler, and E. Hohenester Laminin: the crux of basement membrane assembly J. Cell Biol., March 29, 2004; 164(7): 959 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, D. Harrison, S. Carbonetto, R. Fassler, N. Smyth, D. Edgar, and P. D. Yurchenco Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation J. Cell Biol., June 24, 2002; 157(7): 1279 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Smirnov, E. L. McDearmon, S. Li, J. M. Ervasti, K. Tryggvason, and P. D. Yurchenco Contributions of the LG Modules and Furin Processing to Laminin-2 Functions J. Biol. Chem., May 17, 2002; 277(21): 18928 - 18937. [Abstract] [Full Text] [PDF]
|
|
