|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 5 941-952, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
GK Ojakian, DR Ratcliffe and R Schwimmer
Department of Anatomy and Cell Biology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA. gojakian@netmail.hscbklyn.edu
The extracellular matrix plays an important role in regulation of epithelial development and organization. To determine more precisely the function of extracellular matrix in this process, the initial steps in collagen-mediated formation of epithelial tubules were studied using a model cell culture system. Previous studies have demonstrated that incubation of Madin-Darby canine kidney (MDCK) epithelial cells with a collagen gel overlay induces (beta)1 integrin-regulated epithelial remodeling accompanied by extensive cell rearrangements and formation of epithelial tubules. During epithelial remodeling there was extensive disruption of the epithelial junctional complex. Progressive opening of tight junctions was observed over 8 hours using transepithelial resistance measurements and immunofluorescence microscopy demonstrated that tight and adherens junction proteins were dispersed throughout the apical and basolateral membranes. Junction complex disruption allowed the formation of apical cell extensions and subsequent migration of selected cell sheets from the epithelial monolayer. Confocal microscopy demonstrated the presence of adherens junction (E-cadherin, (alpha)-catenin, (beta)-catenin, plakoglobin) and desmosomal (desmoplakin-1/2, plakoglobin) proteins on, and within, cell extensions demonstrating that cell junctions had undergone considerable disassembly. However, groups of cell extensions appeared to be associated by E-cadherin/catenin-mediated interactions. Association of E-cadherin/catenin complexes with the epithelial cytoskeleton was analyzed by differential detergent extraction. SDS-PAGE and immunoblot analysis demonstrated that adherens junction proteins were primarily cytoskeleton-associated in control cells. During integrin-regulated remodeling, there was a progressive reduction in the interaction of adherens junction proteins with the cytoskeleton suggesting that they play an important role in the maintenance of epithelial integrity. Since loss of transepithelial electrical resistance and disruption of junctional complexes were inhibited by an antifunctional integrin antibody, we propose that activation of integrin signaling pathways regulate junctional complex stability, cell-cell interactions and cell migration. These observations provide evidence that integrin-regulated MDCK epithelial tubule formation can serve as a model system for studying rearrangements of epithelial sheets which occur during development.
This article has been cited by other articles:
|
|
 |
|
  J. M. Torkko, A. Manninen, S. Schuck, and K. Simons Depletion of apical transport proteins perturbs epithelial cyst formation and ciliogenesis J. Cell Sci., April 15, 2008; 121(8): 1193 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thery, V. Racine, M. Piel, A. Pepin, A. Dimitrov, Y. Chen, J.-B. Sibarita, and M. Bornens From the Cover: Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity PNAS, December 26, 2006; 103(52): 19771 - 19776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Battini, E. Fedorova, S. Macip, X. Li, P. D. Wilson, and G. L. Gusella Stable Knockdown of Polycystin-1 Confers Integrin-{alpha}2beta1-Mediated Anoikis Resistance J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3049 - 3058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamanaka, Y. Horikoshi, N. Izumi, A. Suzuki, K. Mizuno, and S. Ohno Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity J. Cell Sci., May 15, 2006; 119(10): 2107 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Eisen, S. Walid, D. R. Ratcliffe, and G. K. Ojakian Regulation of epithelial tubule formation by Rho family GTPases Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1297 - C1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Park, H. Zhang, M. Pallavicini, J. W. Gray, F. Baehner, C. J. Park, and M. J. Bissell {beta}1 Integrin Inhibitory Antibody Induces Apoptosis of Breast Cancer Cells, Inhibits Growth, and Distinguishes Malignant from Normal Phenotype in Three Dimensional Cultures and In vivo Cancer Res., February 1, 2006; 66(3): 1526 - 1535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vespa, S. J.A. D'Souza, and L. Dagnino A Novel Role for Integrin-linked Kinase in Epithelial Sheet Morphogenesis Mol. Biol. Cell, September 1, 2005; 16(9): 4084 - 4095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Haga, C. Irahara, R. Kobayashi, T. Nakagaki, and K. Kawabata Collective Movement of Epithelial Cells on a Collagen Gel Substrate Biophys. J., March 1, 2005; 88(3): 2250 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, A. Datta, P. Leroy, L. E. O'Brien, G. Mak, T.-S. Jou, K. S. Matlin, K. E. Mostov, and M. M.P. Zegers {beta}1-Integrin Orients Epithelial Polarity via Rac1 and Laminin Mol. Biol. Cell, February 1, 2005; 16(2): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eisen, D. R. Ratcliffe, and G. K. Ojakian Modulation of epithelial tubule formation by Rho kinase Am J Physiol Cell Physiol, April 1, 2004; 286(4): C857 - C866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cohen, P. J. Brennwald, E. Rodriguez-Boulan, and A. Musch Mammalian PAR-1 determines epithelial lumen polarity by organizing the microtubule cytoskeleton J. Cell Biol., March 1, 2004; 164(5): 717 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Perego, C. Vanoni, S. Massari, A. Raimondi, S. Pola, M. G. Cattaneo, M. Francolini, L. M. Vicentini, and G. Pietrini Invasive behaviour of glioblastoma cell lines is associated with altered organisation of the cadherin-catenin adhesion system J. Cell Sci., August 15, 2002; 115(16): 3331 - 3340. [Abstract] [Full Text] [PDF]
|
|
