spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Self, A. J.
Right arrow Articles by Hall, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Self, A. J.
Right arrow Articles by Hall, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 114, Issue 7 1357-1366, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Analysis of R-Ras signalling pathways

AJ Self, E Caron, HF Paterson and A Hall
MRC Laboratory for Molecular Cell Biology, CRC Oncogene and Signal Transduction Group and Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, UK. Alan.Hall@ucl.ac.uk

R-Ras has a high degree of sequence homology to Ras and to other members of the Ras subfamily including Rap, TC21 and M-Ras. Activated versions of Ras and TC21 are highly transforming in a variety of cell lines and mutated forms of both proteins have been found in human tumours. R-Ras interacts with many of the same proteins as Ras and TC21, including c-Raf1, and can induce transformed foci, although this activity is weak compared to Ras and appears to be cell-type specific. Here, we have investigated R-Ras signalling pathways in a variety of cell types. We find that microinjection of activated R-Ras into quiescent fibroblasts stimulates cell cycle progression through G(1) phase and subsequent DNA synthesis. However, unlike Ras, R-Ras does not activate the ERK MAP kinase pathway nor does it activate the JNK or p38/Mpk2 MAP kinase pathways. Microinjection of R-Ras into PC12 cells does not induce terminal differentiation, but instead causes extensive cell spreading, consistent with R-Ras having a role in integrin activation. Finally, in a macrophage cell line, R-Ras activates the (&agr;)(M)(&bgr;)(2 )integrin via the small GTPase Rap1, leading to phagocytosis of opsonized red blood cells, whereas Ras does not. These results indicate that R-Ras has an important role in the regulation of cell growth and adhesion, but that this is mediated through downstream signals distinct from those used by Ras.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. R. Cera, M. Fabbri, C. Molendini, M. Corada, F. Orsenigo, M. Rehberg, C. A. Reichel, F. Krombach, R. Pardi, and E. Dejana
JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling
J. Cell Sci., January 15, 2009; 122(2): 268 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. W-M. Gho, W.-K. Ip, K. Y-Y. Chan, P. T-Y. Law, P. B-S. Lai, and N. Wong
Re-Expression of Transcription Factor ATF5 in Hepatocellular Carcinoma Induces G2-M Arrest
Cancer Res., August 15, 2008; 68(16): 6743 - 6751.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Erdogan, A. Pozzi, N. Bhowmick, H. L. Moses, and R. Zent
Transforming Growth Factor-{beta} (TGF-{beta}) and TGF-{beta}-Associated Kinase 1 Are Required for R-Ras-Mediated Transformation of Mammary Epithelial Cells
Cancer Res., August 1, 2008; 68(15): 6224 - 6231.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G. Serini, L. Napione, M. Arese, and F. Bussolino
Besides adhesion: new perspectives of integrin functions in angiogenesis
Cardiovasc Res, May 1, 2008; 78(2): 213 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
N. El Zein, B. Badran, and E. Sariban
VIP differentially activates {beta}2 integrins, CR1, and matrix metalloproteinase-9 in human monocytes through cAMP/PKA, EPAC, and PI-3K signaling pathways via VIP receptor type 1 and FPRL1
J. Leukoc. Biol., April 1, 2008; 83(4): 972 - 981.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Lehto, M. I. Mayranpaa, T. Pellinen, P. Ihalmo, S. Lehtonen, P. T. Kovanen, P.-H. Groop, J. Ivaska, and V. M. Olkkonen
The R-Ras interaction partner ORP3 regulates cell adhesion
J. Cell Sci., March 1, 2008; 121(5): 695 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. H. Serezani, J. Chung, M. N. Ballinger, B. B. Moore, D. M. Aronoff, and M. Peters-Golden
Prostaglandin E2 Suppresses Bacterial Killing in Alveolar Macrophages by Inhibiting NADPH Oxidase
Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 562 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Stasyk, N. Schiefermeier, S. Skvortsov, H. Zwierzina, J. Peranen, G. K. Bonn, and L. A. Huber
Identification of Endosomal Epidermal Growth Factor Receptor Signaling Targets by Functional Organelle Proteomics
Mol. Cell. Proteomics, May 1, 2007; 6(5): 908 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Takaya, T. Kamio, M. Masuda, N. Mochizuki, H. Sawa, M. Sato, K. Nagashima, A. Mizutani, A. Matsuno, E. Kiyokawa, et al.
R-Ras Regulates Exocytosis by Rgl2/Rlf-mediated Activation of RalA on Endosomes
Mol. Biol. Cell, May 1, 2007; 18(5): 1850 - 1860.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Iwashita, M. Kobayashi, Y. Kubo, Y. Hinohara, M. Sezaki, K. Nakamura, R. Suzuki-Migishima, M. Yokoyama, S. Sato, M. Fukuda, et al.
Versatile Roles of R-Ras GAP in Neurite Formation of PC12 Cells and Embryonic Vascular Development
J. Biol. Chem., February 9, 2007; 282(6): 3413 - 3417.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. E. Goldfinger, C. Ptak, E. D. Jeffery, J. Shabanowitz, D. F. Hunt, and M. H. Ginsberg
RLIP76 (RalBP1) is an R-Ras effector that mediates adhesion-dependent Rac activation and cell migration
J. Cell Biol., September 11, 2006; 174(6): 877 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Yu, Y. Hao, and L. A. Feig
The R-Ras GTPase Mediates Cross Talk between Estrogen and Insulin Signaling in Breast Cancer Cells.
Mol. Cell. Biol., September 1, 2006; 26(17): 6372 - 6380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. L. De Jesus, M. B. Stope, P. A. O. Weernink, Y. Mahlke, C. Borgermann, V. N. Ananaba, C. Rimmbach, D. Rosskopf, M. C. Michel, K. H. Jakobs, et al.
Cyclic AMP-dependent and Epac-mediated Activation of R-Ras by G Protein-coupled Receptors Leads to Phospholipase D Stimulation
J. Biol. Chem., August 4, 2006; 281(31): 21837 - 21847.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Dail, M. Richter, P. Godement, and E. B. Pasquale
Eph receptors inactivate R-Ras through different mechanisms to achieve cell repulsion
J. Cell Sci., April 1, 2006; 119(7): 1244 - 1254.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. S. Ada-Nguema, H. Xenias, M. P. Sheetz, and P. J. Keely
The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLC{epsilon}
J. Cell Sci., April 1, 2006; 119(7): 1307 - 1319.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. J. S. Stork and T. J. Dillon
Multiple roles of Rap1 in hematopoietic cells: complementary versus antagonistic functions
Blood, November 1, 2005; 106(9): 2952 - 2961.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Nakada, J. A. Niska, N. L. Tran, W. S. McDonough, and M. E. Berens
EphB2/R-Ras Signaling Regulates Glioma Cell Adhesion, Growth, and Invasion
Am. J. Pathol., August 1, 2005; 167(2): 565 - 576.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. P. Holly, M. K. Larson, and L. V. Parise
The Unique N-Terminus of R-Ras Is Required for Rac Activation and Precise Regulation of Cell Migration
Mol. Biol. Cell, May 1, 2005; 16(5): 2458 - 2469.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G.-X. Shi and D. A. Andres
Rit Contributes to Nerve Growth Factor-Induced Neuronal Differentiation via Activation of B-Raf-Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Cascades
Mol. Cell. Biol., January 15, 2005; 25(2): 830 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H.-W. Jeong, J.-O. Nam, and I.-S. Kim
The COOH-terminal End of R-Ras Alters the Motility and Morphology of Breast Epithelial Cells through Rho/Rho-Kinase
Cancer Res., January 15, 2005; 65(2): 507 - 515.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. A. Wozniak, L. Kwong, D. Chodniewicz, R. L. Klemke, and P. J. Keely
R-Ras Controls Membrane Protrusion and Cell Migration through the Spatial Regulation of Rac and Rho
Mol. Biol. Cell, January 1, 2005; 16(1): 84 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Rodriguez-Viciana, C. Sabatier, and F. McCormick
Signaling Specificity by Ras Family GTPases Is Determined by the Full Spectrum of Effectors They Regulate
Mol. Cell. Biol., June 1, 2004; 24(11): 4943 - 4954.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Furuhjelm and J. Peranen
The C-terminal end of R-Ras contains a focal adhesion targeting signal
J. Cell Sci., September 15, 2003; 116(18): 3729 - 3738.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Z. Wang, T. M. Leisner, and L. V. Parise
Platelet {alpha}2{beta}1 integrin activation: contribution of ligand internalization and the {alpha}2-cytoplasmic domain
Blood, August 15, 2003; 102(4): 1307 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. L. Cole, G. Subbanagounder, S. Mukhopadhyay, J. A. Berliner, and D. K. Vora
Oxidized Phospholipid-Induced Endothelial Cell/Monocyte Interaction Is Mediated by a cAMP-Dependent R-Ras/PI3-Kinase Pathway
Arterioscler Thromb Vasc Biol, August 1, 2003; 23(8): 1384 - 1390.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. J. H. Koopman, R. R. Bosch, S. E. van Emst-de Vries, M. Spaargaren, J. J. H. H. M. De Pont, and P. H. G. M. Willems
R-Ras Alters Ca2+ Homeostasis by Increasing the Ca2+ Leak across the Endoplasmic Reticular Membrane
J. Biol. Chem., April 11, 2003; 278(16): 13672 - 13679.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Kwong, M. A. Wozniak, A. S. Collins, S. D. Wilson, and P. J. Keely
R-Ras Promotes Focal Adhesion Formation through Focal Adhesion Kinase and p130Cas by a Novel Mechanism That Differs from Integrins
Mol. Cell. Biol., February 1, 2003; 23(3): 933 - 949.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
E. Caron
Cellular functions of the Rap1 GTP-binding protein: a pattern emerges
J. Cell Sci., February 1, 2003; 116(3): 435 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bertoni, S. Tadokoro, K. Eto, N. Pampori, L. V. Parise, G. C. White, and S. J. Shattil
Relationships between Rap1b, Affinity Modulation of Integrin alpha IIbbeta 3, and the Actin Cytoskeleton
J. Biol. Chem., July 5, 2002; 277(28): 25715 - 25721.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Tian and L. A. Feig
Basis for Signaling Specificity Difference between Sos and Ras-GRF Guanine Nucleotide Exchange Factors
J. Biol. Chem., December 7, 2001; 276(50): 47248 - 47256.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2001