|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 114, Issue 8 1491-1503, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
CH Herrmann and MA Mancini
Dept of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA. herrmann@bcm.tmc.edu
TAK/P-TEFb is an elongation factor for RNA polymerase II-directed transcription that is thought to function by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. TAK/P-TEFb is composed of Cdk9 and cyclin T and serves as the cellular cofactor for the human immunodeficiency virus transactivator Tat protein. In this study, we examined the subcellular distribution of Cdk9 and cyclin T1 using high resolution immunofluorescence microscopy and found that Cdk9 and cyclin T1 localized throughout the non-nucleolar nucleoplasm, with increased signal present at numerous foci. Both Cdk9 and cyclin T1 showed only limited colocalization with different phosphorylated forms of RNA polymerase II. However, significant colocalization with antibodies to several splicing factors that identify nuclear 'speckles' was observed for Cdk9 and especially for cyclin T1. The pattern of Cdk9 and cyclin T1 distribution was altered in cells treated with transcription inhibitors. Transient expression of cyclin T1 deletion mutants indicated that a region in the central portion of cyclin T1 is important for accumulation at speckles. Furthermore, cyclin T1 proteins that accumulated at speckles were capable of recruiting Cdk9 and the HIV Tat protein to this compartment in overexpression experiments. These results suggest that cyclin T1 functions to recruit its binding partners to nuclear speckles and raises the possibility that nuclear speckles are a site of TAK/P-TEFb function.
This article has been cited by other articles:
|
|
 |
|
  S. J. Leuenroth and C. M. Crews Triptolide-Induced Transcriptional Arrest Is Associated with Changes in Nuclear Substructure Cancer Res., July 1, 2008; 68(13): 5257 - 5266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Oven, N. Brdickova, J. Kohoutek, T. Vaupotic, M. Narat, and B. M. Peterlin AIRE Recruits P-TEFb for Transcriptional Elongation of Target Genes in Medullary Thymic Epithelial Cells Mol. Cell. Biol., December 15, 2007; 27(24): 8815 - 8823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Espinoza-Derout, M. Wagner, K. Shahmiri, E. Mascareno, B. Chaqour, and M.A.Q. Siddiqui Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy Cardiovasc Res, July 1, 2007; 75(1): 129 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Barboric, J. H. N. Yik, N. Czudnochowski, Z. Yang, R. Chen, X. Contreras, M. Geyer, B. Matija Peterlin, and Q. Zhou Tat competes with HEXIM1 to increase the active pool of P-TEFb for HIV-1 transcription Nucleic Acids Res., March 19, 2007; 35(6): 2003 - 2012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sanchez-Alvarez, A. C. Goldstrohm, M. A. Garcia-Blanco, and C. Sune Human Transcription Elongation Factor CA150 Localizes to Splicing Factor-Rich Nuclear Speckles and Assembles Transcription and Splicing Components into Complexes through Its Amino and Carboxyl Regions. Mol. Cell. Biol., July 1, 2006; 26(13): 4998 - 5014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Q. Xie, S. Martin, P. V. Guillot, D. L. Bentley, and A. Pombo Splicing Speckles Are Not Reservoirs of RNA Polymerase II, but Contain an Inactive Form, Phosphorylated on Serine2 Residues of the C-Terminal Domain Mol. Biol. Cell, April 1, 2006; 17(4): 1723 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O. Durand, S. J. Advani, A. P. W. Poon, and B. Roizman The Carboxyl-Terminal Domain of RNA Polymerase II Is Phosphorylated by a Complex Containing cdk9 and Infected-Cell Protein 22 of Herpes Simplex Virus 1 J. Virol., June 1, 2005; 79(11): 6757 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bres, N. Gomes, L. Pickle, and K. A. Jones A human splicing factor, SKIP, associates with P-TEFb and enhances transcription elongation by HIV-1 Tat Genes & Dev., May 15, 2005; 19(10): 1211 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Peng, S. C. Moses, J. Tan, E. Kremmer, and P. D. Ling The Epstein-Barr Virus EBNA-LP Protein Preferentially Coactivates EBNA2-Mediated Stimulation of Latent Membrane Proteins Expressed from the Viral Divergent Promoter J. Virol., April 1, 2005; 79(7): 4492 - 4505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kavanagh, T. C. Schulz, P. Davey, C. Claudianos, C. Russell, and P. D. Rathjen A family of RS domain proteins with novel subcellular localization and trafficking Nucleic Acids Res., March 1, 2005; 33(4): 1309 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Smith, S. Kulkarni, and T. Pawson FF Domains of CA150 Bind Transcription and Splicing Factors through Multiple Weak Interactions Mol. Cell. Biol., November 1, 2004; 24(21): 9274 - 9285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Diederichs, N. Baumer, P. Ji, S. K. Metzelder, G. E. Idos, T. Cauvet, W. Wang, M. Moller, S. Pierschalski, J. Gromoll, et al. Identification of Interaction Partners and Substrates of the Cyclin A1-CDK2 Complex J. Biol. Chem., August 6, 2004; 279(32): 33727 - 33741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. de Graaf, P. Hekerman, O. Spelten, A. Herrmann, L. C. Packman, K. Bussow, G. Muller-Newen, and W. Becker Characterization of Cyclin L2, a Novel Cyclin with an Arginine/Serine-rich Domain: PHOSPHORYLATION BY DYRK1A AND COLOCALIZATION WITH SPLICING FACTORS J. Biol. Chem., February 6, 2004; 279(6): 4612 - 4624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Young, Q. Wang, T. Pe'ery, and M. B. Mathews The Human I-mfa Domain-Containing Protein, HIC, Interacts with Cyclin T1 and Modulates P-TEFb-Dependent Transcription Mol. Cell. Biol., September 15, 2003; 23(18): 6373 - 6384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Alvarez, X. Estivill, and S. de la Luna DYRK1A accumulates in splicing speckles through a novel targeting signal and induces speckle disassembly J. Cell Sci., August 1, 2003; 116(15): 3099 - 3107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Michels, V. T. Nguyen, A. Fraldi, V. Labas, M. Edwards, F. Bonnet, L. Lania, and O. Bensaude MAQ1 and 7SK RNA Interact with CDK9/Cyclin T Complexes in a Transcription-Dependent Manner Mol. Cell. Biol., July 15, 2003; 23(14): 4859 - 4869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. R. K. Yedavalli, M. Benkirane, and K.-T. Jeang Tat and Trans-activation-responsive (TAR) RNA-independent Induction of HIV-1 Long Terminal Repeat by Human and Murine Cyclin T1 Requires Sp1 J. Biol. Chem., February 14, 2003; 278(8): 6404 - 6410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-Y. Liou, C. H. Herrmann, and A. P. Rice Transient Induction of Cyclin T1 during Human Macrophage Differentiation Regulates Human Immunodeficiency Virus Type 1 Tat Transactivation Function J. Virol., October 2, 2002; 76(21): 10579 - 10587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, J. Li, B. O. Kim, B. S. Pace, and J. J. He HIV-1 Tat Protein-mediated Transactivation of the HIV-1 Long Terminal Repeat Promoter Is Potentiated by a Novel Nuclear Tat-interacting Protein of 110 kDa, Tip110 J. Biol. Chem., June 21, 2002; 277(26): 23854 - 23863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Vandepoele, J. Raes, L. De Veylder, P. Rouze, S. Rombauts, and D. Inze Genome-Wide Analysis of Core Cell Cycle Genes in Arabidopsis PLANT CELL, April 1, 2002; 14(4): 903 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hoque, T. M. Young, C.-G. Lee, G. Serrero, M. B. Mathews, and T. Pe'ery The Growth Factor Granulin Interacts with Cyclin T1 and Modulates P-TEFb-Dependent Transcription Mol. Cell. Biol., March 1, 2002; 23(5): 1688 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Pendergrast, C. Wang, N. Hernandez, and S. Huang FBI-1 Can Stimulate HIV-1 Tat Activity and Is Targeted to a Novel Subnuclear Domain that Includes the Tat-P-TEFb---containing Nuclear Speckles Mol. Biol. Cell, March 1, 2002; 13(3): 915 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martin-Serrano, K. Li, and P. D. Bieniasz Cyclin T1 Expression Is Mediated by a Complex and Constitutively Active Promoter and Does Not Limit Human Immunodeficiency Virus Type 1 Tat Function in Unstimulated Primary Lymphocytes J. Virol., January 1, 2002; 76(1): 208 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ghose, L.-Y. Liou, C. H. Herrmann, and A. P. Rice Induction of TAK (Cyclin T1/P-TEFb) in Purified Resting CD4+ T Lymphocytes by Combination of Cytokines J. Virol., December 1, 2001; 75(23): 11336 - 11343. [Abstract] [Full Text]
|
|
|
|
|
|
A. Marcello, R. A. G. Cinelli, A. Ferrari, A. Signorelli, M. Tyagi, V. Pellegrini, F. Beltram, and M. Giacca Visualization of in Vivo Direct Interaction between HIV-1 TAT and Human Cyclin T1 in Specific Subcellular Compartments by Fluorescence Resonance Energy Transfer J. Biol. Chem., October 12, 2001; 276(42): 39220 - 39225. [Abstract] [Full Text] [PDF]
|
|
