Journal of Cell Science, Vol 114, Issue 8 1533-1544, Copyright © 2001 by Company of Biologists

JOURNAL ARTICLES

Mechanism for the transcriptional repression by c-Myc on PDGF (&bgr;)-receptor

H Izumi, C Molander, LZ Penn, A Ishisaki, K Kohno and K Funa
Department of Cell Biology, Institute of Anatomy and Cell Biology, Goteborg University, Box 420, SE-405 30, Gothenburg, Sweden. keiko.funa@anatcell.gu.se

c-Myc plays a key role in the cell cycle dependent control of the PDGF (&bgr;)-receptor mRNA. The mouse platelet-derived growth factor (PDGF) (&bgr;)-receptor promoter contains a CCAAT motif, and NF-Y plays an essential role in its transcription. Coexpression of c-Myc represses PDGF (&bgr;)-receptor luciferase reporter activity, and the CCAAT motif in the promoter is indispensable for this repression. Here we show that c-Myc binds NF-Y subunits, YB and YC, by immunoprecipitation from cotransfected COS-1 cells. The in vitro-translated c-Myc also binds the glutathione S-transferase (GST)-NF-YB fusion protein and GST-NF-YC, but not GST-NF-YA. The most C-terminal region of HAP domains of NF-YB and NF-YC, and the Myc homology boxes, but not the C-terminal bHLHZip domain, are indispensable for the coimmunoprecipitation, and also for the repression of PDGF (&bgr;)-receptor. c-Myc binds NF-Y complex without affecting the efficiency of NF-Y binding to DNA. However, the expression of Myc represses the transcriptional activation of NF-YC when fused to the GAL4 DNA binding domain. Furthermore, this repression was seen only when Myc homology boxes are present, and NF-YC contains the c-Myc binding region.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				Z. Li, D. Boone, and S. R. Hann Nucleophosmin interacts directly with c-Myc and controls c-Myc-induced hyperproliferation and transformation PNAS, December 2, 2008; 105(48): 18794 - 18799. [Abstract] [Full Text] [PDF]

				M. Eilers and R. N. Eisenman Myc's broad reach Genes & Dev., October 15, 2008; 22(20): 2755 - 2766. [Abstract] [Full Text] [PDF]

				G. Jiang, A. Espeseth, D. J. Hazuda, and D. M. Margolis c-Myc and Sp1 Contribute to Proviral Latency by Recruiting Histone Deacetylase 1 to the Human Immunodeficiency Virus Type 1 Promoter J. Virol., October 15, 2007; 81(20): 10914 - 10923. [Abstract] [Full Text] [PDF]

				S.-Y. Woo, D.-H. Kim, C.-B. Jun, Y.-M. Kim, E. V. Haar, S.-i. Lee, J. W. Hegg, S. Bandhakavi, T. J. Griffin, and D.-H. Kim PRR5, a Novel Component of mTOR Complex 2, Regulates Platelet-derived Growth Factor Receptor beta Expression and Signaling J. Biol. Chem., August 31, 2007; 282(35): 25604 - 25612. [Abstract] [Full Text] [PDF]

				S.-H. Park, G.-R. Yu, W.-H. Kim, W.-S. Moon, J.-H. Kim, and D.-G. Kim NF-Y-Dependent Cyclin B2 Expression in Colorectal Adenocarcinoma Clin. Cancer Res., February 1, 2007; 13(3): 858 - 867. [Abstract] [Full Text] [PDF]

				J. H. Patel and S. B. McMahon Targeting of Miz-1 Is Essential for Myc-mediated Apoptosis J. Biol. Chem., February 10, 2006; 281(6): 3283 - 3289. [Abstract] [Full Text] [PDF]

				G. Brady, L. Boggan, A. Bowie, and L. A. J. O'Neill Schlafen-1 Causes a Cell Cycle Arrest by Inhibiting Induction of Cyclin D1 J. Biol. Chem., September 2, 2005; 280(35): 30723 - 30734. [Abstract] [Full Text] [PDF]

				F. Li, Y. Wang, K. I. Zeller, J. J. Potter, D. R. Wonsey, K. A. O'Donnell, J.-w. Kim, J. T. Yustein, L. A. Lee, and C. V. Dang Myc Stimulates Nuclearly Encoded Mitochondrial Genes and Mitochondrial Biogenesis Mol. Cell. Biol., July 15, 2005; 25(14): 6225 - 6234. [Abstract] [Full Text] [PDF]

				C. Imbriano, A. Gurtner, F. Cocchiarella, S. Di Agostino, V. Basile, M. Gostissa, M. Dobbelstein, G. Del Sal, G. Piaggio, and R. Mantovani Direct p53 Transcriptional Repression: In Vivo Analysis of CCAAT-Containing G2/M Promoters Mol. Cell. Biol., May 1, 2005; 25(9): 3737 - 3751. [Abstract] [Full Text] [PDF]

				A. Testa, G. Donati, P. Yan, F. Romani, T. H.-M. Huang, M. A. Vigano, and R. Mantovani Chromatin Immunoprecipitation (ChIP) on Chip Experiments Uncover a Widespread Distribution of NF-Y Binding CCAAT Sites Outside of Core Promoters J. Biol. Chem., April 8, 2005; 280(14): 13606 - 13615. [Abstract] [Full Text] [PDF]

				H. Uramoto, D. Wetterskog, A. Hackzell, Y. Matsumoto, and K. Funa p73 competes with co-activators and recruits histone deacetylase to NF-Y in the repression of PDGF {beta}-receptor J. Cell Sci., October 15, 2004; 117(22): 5323 - 5331. [Abstract] [Full Text] [PDF]

				R. Elkon, K. I. Zeller, C. Linhart, C. V. Dang, R. Shamir, and Y. Shiloh In silico identification of transcriptional regulators associated with c-Myc Nucleic Acids Res., September 23, 2004; 32(17): 4955 - 4961. [Abstract] [Full Text] [PDF]

				H. Uramoto, A. Hackzell, D. Wetterskog, A. Ballagi, H. Izumi, and K. Funa pRb, Myc and p53 are critically involved in SV40 large T antigen repression of PDGF {beta}-receptor transcription J. Cell Sci., August 1, 2004; 117(17): 3855 - 3865. [Abstract] [Full Text] [PDF]

				Q.-S. Zhu, B. Qian, and D. Levy CCAAT/Enhancer-binding Protein {alpha} (C/EBP{alpha}) Activates Transcription of the Human Microsomal Epoxide Hydrolase Gene (EPHX1) through the Interaction with DNA-bound NF-Y J. Biol. Chem., July 16, 2004; 279(29): 29902 - 29910. [Abstract] [Full Text] [PDF]

				D. Y. L. Mao, D. Barsyte-Lovejoy, C. S. W. Ho, J. D. Watson, A. Stojanova, and L. Z. Penn Promoter-binding and repression of PDGFRB by c-Myc are separable activities Nucleic Acids Res., June 29, 2004; 32(11): 3462 - 3468. [Abstract] [Full Text] [PDF]

				G. Caretti, V. Salsi, C. Vecchi, C. Imbriano, and R. Mantovani Dynamic Recruitment of NF-Y and Histone Acetyltransferases on Cell-cycle Promoters J. Biol. Chem., August 15, 2003; 278(33): 30435 - 30440. [Abstract] [Full Text] [PDF]

				F. Yi, R. Jaffe, and E. V. Prochownik The CCL6 Chemokine Is Differentially Regulated by c-Myc and L-Myc, and Promotes Tumorigenesis and Metastasis Cancer Res., June 1, 2003; 63(11): 2923 - 2932. [Abstract] [Full Text] [PDF]

				Y. Li, X. Li, and F. H. Sarkar Gene Expression Profiles of I3C- and DIM-Treated PC3 Human Prostate Cancer Cells Determined by cDNA Microarray Analysis J. Nutr., April 1, 2003; 133(4): 1011 - 1019. [Abstract] [Full Text] [PDF]

				P. M. Gowri, J. H. Yu, A. Shaufl, M. A. Sperling, and R. K. Menon Recruitment of a Repressosome Complex at the Growth Hormone Receptor Promoter and Its Potential Role in Diabetic Nephropathy Mol. Cell. Biol., February 1, 2003; 23(3): 815 - 825. [Abstract] [Full Text]

				C. Romier, F. Cocchiarella, R. Mantovani, and D. Moras The NF-YB/NF-YC Structure Gives Insight into DNA Binding and Transcription Regulation by CCAAT Factor NF-Y J. Biol. Chem., January 3, 2003; 278(2): 1336 - 1345. [Abstract] [Full Text] [PDF]

				A. Hackzell, H. Uramoto, H. Izumi, K. Kohno, and K. Funa p73 Independent of c-Myc Represses Transcription of Platelet-derived Growth Factor beta -Receptor through Interaction with NF-Y J. Biol. Chem., October 11, 2002; 277(42): 39769 - 39776. [Abstract] [Full Text] [PDF]

				J. D. Watson, S. K. Oster, M. Shago, F. Khosravi, and L. Z. Penn Identifying Genes Regulated in a Myc-dependent Manner J. Biol. Chem., September 27, 2002; 277(40): 36921 - 36930. [Abstract] [Full Text] [PDF]

				H. Uramoto, H. Izumi, T. Ise, M. Tada, T. Uchiumi, M. Kuwano, K. Yasumoto, K. Funa, and K. Kohno p73 Interacts with c-Myc to Regulate Y-box-binding Protein-1 Expression J. Biol. Chem., August 23, 2002; 277(35): 31694 - 31702. [Abstract] [Full Text] [PDF]

				K. Kamemura, B. K. Hayes, F. I. Comer, and G. W. Hart Dynamic Interplay between O-Glycosylation and O-Phosphorylation of Nucleocytoplasmic Proteins. ALTERNATIVE GLYCOSYLATION/PHOSPHORYLATION OF THR-58, A KNOWN MUTATIONAL HOT SPOT OF c-Myc IN LYMPHOMAS, IS REGULATED BY MITOGENS J. Biol. Chem., May 17, 2002; 277(21): 19229 - 19235. [Abstract] [Full Text] [PDF]