spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    


This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, J. H.
Right arrow Articles by Wu, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, J. H.
Right arrow Articles by Wu, S. M.

Journal of Cell Science, Vol 114, Issue 9 1655-1664, Copyright © 2001 by Company of Biologists


JOURNAL ARTICLES

Apoptotic cell death of cultured salamander photoreceptors induced by cccp: CsA-insensitive mitochondrial permeability transition

JH Yang, RL Gross, SF Basinger and SM Wu
Dept of Ophthalmology, Baylor College of Medicine, NC205, Houston, TX 77030-2702, USA. jhyang@bcm.tmc.edu

Photoreceptor degeneration is mediated by apoptosis in several animal models, although the underlying mechanisms are yet to be elucidated. We present here an apoptotic model based on a primary cell culture of tiger salamander photoreceptors, in which treatment with carbonyl cyanide m-chlorophenylhydrazone (cccp), a protonophore, induced apoptosis. Cells exposed to cccp showed condensed nuclei and displayed positive TdT-dUTP terminal nick-end labeling (TUNEL). In addition, 10-100 microM cccp rapidly induced a reduction of Delta psi(m) and > or = 30 microM cccp induced a significant leakage of calcein from mitochondria to cytosol and nucleus, indicating a change in mitochondrial inner membrane permeability. Cyclosporin A (CsA), a transition pore blocker, did not prevent the cccp-induced MPT or the cccp-evoked apoptotic cell death, suggesting that cccp-induced apoptotic process was mediated by a CsA-insensitive pathway. This cell model provides an in vitro tool for studying mechanisms of photoreceptor apoptosis in isolated photoreceptors and may provide clues to the etiology of retinal degeneration.


This article has been cited by other articles:


Home page
J. Biol. Chem.Home page
J. S. Armstrong, H. Yang, W. Duan, and M. Whiteman
Cytochrome bc1 Regulates the Mitochondrial Permeability Transition by Two Distinct Pathways
J. Biol. Chem., November 26, 2004; 279(48): 50420 - 50428.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
W. Bergmeier, P. C. Burger, C. L. Piffath, K. M. Hoffmeister, J. H. Hartwig, B. Nieswandt, and D. D. Wagner
Metalloproteinase inhibitors improve the recovery and hemostatic function of in vitro-aged or -injured mouse platelets
Blood, December 1, 2003; 102(12): 4229 - 4235.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2001