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Journal of Cell Science, Vol 114, Issue 9 1699-1708, Copyright © 2001 by Company of Biologists
JOURNAL ARTICLES |
MD Haskell, AL Nickles, JM Agati, L Su, BD Dukes and SJ Parsons
Department of Microbiology and Cancer Center, Box 800734, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA. sap@virginia.edu
p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 participates in actin cytoskeleton rearrangements that occur following transformation by v-Src or stimulation by growth factors, and that tyrosine phosphorylation of p190 by Src influences these processes. The current study was performed to establish whether p190RhoGAP directly participates in epidermal growth factor-induced actin stress fiber disassembly and how c-Src is involved in this process. Our results support a model in which the p190 MD negatively regulates the activity of the GAP domain and that c-Src phosphorylation of Y1105 is necessary, but insufficient on its own, for actin stress fiber disassembly.
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