|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Research Article |
Institut Jacques Monod, CNRS-UMRC9922, Université Paris 6 and Paris 7-Denis Diderot, 2 place Jussieu, 75251-Paris-cedex 05, France
* These authors contributed equally to this work
Author for correspondence (e-mail: grimal{at}ijm.jussieu.fr)
Accepted September 26, 2001
The modification of yeast uracil permease by phosphorylation at the plasma membrane is a key mechanism for regulating transporter endocytosis. Uracil permease is phosphorylated at several serine residues within a well characterized PEST sequence. The phosphorylation of these residues facilitates the ubiquitination and internalization of the permease. Following endocytosis, the permease is targeted to the lysosome/vacuole for proteolysis. We have shown that in casein kinase 1 (CK1)-deficient cells, the permease is poorly phosphorylated, poorly ubiquitinated and that Yck activity may play a direct role in phosphorylating the permease. We show here that CK1-deficient cells accumulated permease that was subjected to endocytosis in an internal compartment on its way to the vacuole. Uracil permease, produced as a fusion protein with green fluorescent protein in CK1-deficient cells, was detected in dots adjacent to the vacuole. These dots probably correspond to the late endosome/prevacuolar compartment because they were partially colocalized with the Pep12p marker. This accumulation was abolished by mutations affecting the adaptor-related complex, AP-3. The CPY and ALP pathways to the vacuole were both unaffected in CK1-deficient cells. Our analysis provides the first evidence that CK1 is important for the delivery of proteins to the vacuole after endocytosis.
Key words: Endocytosis, Saccharomyces cerevisiae, Transporter, Late endosome, Vacuole, Kinase, CK1, AP-3
This article has been cited by other articles:
|
|
 |
|
  C. J. Brame, W. M. Pruitt, and L. C. Robinson A Molecular Genetics Laboratory Course Applying Bioinformatics and Cell Biology in the Context of Original Research CBE Life Sci Educ, December 1, 2008; 7(4): 410 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pineau, L. Bonifait, J.-M. Berjeaud, P. Alimardani-Theuil, T. Berges, and T. Ferreira A Lipid-mediated Quality Control Process in the Golgi Apparatus in Yeast Mol. Biol. Cell, March 1, 2008; 19(3): 807 - 821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dupre-Crochet, A. Figueroa, C. Hogan, E. C. Ferber, C. U. Bialucha, J. Adams, E. C. N. Richardson, and Y. Fujita Casein Kinase 1 Is a Novel Negative Regulator of E-Cadherin-Based Cell-Cell Contacts Mol. Cell. Biol., May 15, 2007; 27(10): 3804 - 3816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kota, M. Melin-Larsson, P. O. Ljungdahl, and H. Forsberg Ssh4, Rcr2 and Rcr1 Affect Plasma Membrane Transporter Activity in Saccharomyces cerevisiae Genetics, April 1, 2007; 175(4): 1681 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gadura, L. C. Robinson, and C. A. Michels Glc7-Reg1 Phosphatase Signals to Yck1,2 Casein Kinase 1 to Regulate Transport Activity and Glucose-Induced Inactivation of Saccharomyces Maltose Permease Genetics, March 1, 2006; 172(3): 1427 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Levin Cell Wall Integrity Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 262 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Johnston, C. Foote, A. Santeford, and S. F. Nothwehr Golgi-to-Late Endosome Trafficking of the Yeast Pheromone Processing Enzyme Ste13p Is Regulated by a Phosphorylation Site in its Cytosolic Domain Mol. Biol. Cell, March 1, 2005; 16(3): 1456 - 1468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Abdel-Sater, M. El Bakkoury, A. Urrestarazu, S. Vissers, and B. Andre Amino Acid Signaling in Yeast: Casein Kinase I and the Ssy5 Endoprotease Are Key Determinants of Endoproteolytic Activation of the Membrane-Bound Stp1 Transcription Factor Mol. Cell. Biol., November 15, 2004; 24(22): 9771 - 9785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bugnicourt, M. Froissard, K. Sereti, H. D. Ulrich, R. Haguenauer-Tsapis, and J.-M. Galan Antagonistic Roles of ESCRT and Vps Class C/HOPS Complexes in the Recycling of Yeast Membrane Proteins Mol. Biol. Cell, September 1, 2004; 15(9): 4203 - 4214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Moriya and M. Johnston Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I PNAS, February 10, 2004; 101(6): 1572 - 1577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-O. Blondel, J. Morvan, S. Dupre, D. Urban-Grimal, R. Haguenauer-Tsapis, and C. Volland Direct Sorting of the Yeast Uracil Permease to the Endosomal System Is Controlled by Uracil Binding and Rsp5p-dependent Ubiquitylation Mol. Biol. Cell, February 1, 2004; 15(2): 883 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Nikko, A.-M. Marini, and B. Andre Permease Recycling and Ubiquitination Status Reveal a Particular Role for Bro1 in the Multivesicular Body Pathway J. Biol. Chem., December 12, 2003; 278(50): 50732 - 50743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hearn, R. L. Lester, and R. C. Dickson The Uracil Transporter Fur4p Associates with Lipid Rafts J. Biol. Chem., January 31, 2003; 278(6): 3679 - 3686. [Abstract] [Full Text] [PDF]
|
|
