|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Research Article |
Institute for Medical Biochemistry,
1 Center for Molecular Biology of Inflammation and
2 Institute of Infectiology University of Münster, von-Esmarch-Str. 56, D-48149 Münster, Germany
* These authors contributed equally to this work
Author for correspondence (e-mail: gerke{at}uni-muenster.de)
Accepted September 26, 2001
Annexin 2 is a Ca2+-regulated membrane- and F-actin-binding protein implicated in the stabilization or regulation of membrane/cytoskeleton contacts, or both, at the plasma membrane and at early endosomal membranes. To analyze the dynamic nature of such action we investigated whether annexin 2 could be found at sites of localized actin rearrangements occurring at the plasma membrane of HeLa cells infected with noninvading enteropathogenic Escherichia coli (EPEC). We show that adherent EPEC microcolonies, which are known to induce the formation of actin-rich pedestals beneath them, specifically recruit annexin 2 to the sites of their attachment. Mutant EPEC (EPECtir), which lack a functional receptor for intimate attachment (Tir, translocated intimin receptor) and which fail to produce full pedestal formation, are still capable of recruiting annexin 2 to the bacterial contact sites. Accumulation of annexin 2 at sites of EPEC or EPECtir attachment is accompanied by a recruitment of the annexin 2 protein ligand S100A10. EPEC and EPECtir attachment also induces a concentration of cholesterol and glycosyl phosphatidylinositol-anchored proteins at sites of bacterial contact. This indicates that membrane components present in rafts or raft-like microdomains are clustered upon EPEC adherence and that annexin 2 is recruited to the cytoplasmic membrane surface of such clusters, possibly stabilizing raft patches and their linkage to the actin cytoskeleton beneath adhering EPEC.
Key words: Actin organization, Calcium, Membrane microdomain, Raft
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  B. A. Babbin, M. Sasaki, K. W. Gerner-Schmidt, A. Nusrat, and J.-M. A. Klapproth The Bacterial Virulence Factor Lymphostatin Compromises Intestinal Epithelial Barrier Function by Modulating Rho GTPases Am. J. Pathol., April 1, 2009; 174(4): 1347 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sason, M. Milgrom, A. M. Weiss, N. Melamed-Book, T. Balla, S. Grinstein, S. Backert, I. Rosenshine, and B. Aroeti Enteropathogenic Escherichia coli Subverts Phosphatidylinositol 4,5-Bisphosphate and Phosphatidylinositol 3,4,5-Trisphosphate upon Epithelial Cell Infection Mol. Biol. Cell, January 1, 2009; 20(1): 544 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Rescher, C. Ludwig, V. Konietzko, A. Kharitonenkov, and V. Gerke Tyrosine phosphorylation of annexin A2 regulates Rho-mediated actin rearrangement and cell adhesion J. Cell Sci., July 1, 2008; 121(13): 2177 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Graauw, I. Tijdens, M. B. Smeets, P. J. Hensbergen, A. M. Deelder, and B. van de Water Annexin A2 Phosphorylation Mediates Cell Scattering and Branching Morphogenesis via Cofilin Activation Mol. Cell. Biol., February 1, 2008; 28(3): 1029 - 1040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Chintagari, N. Jin, P. Wang, T. A. Narasaraju, J. Chen, and L. Liu Effect of Cholesterol Depletion on Exocytosis of Alveolar Type II Cells Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 677 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jandu, P. J. M. Ceponis, S. Kato, J. D. Riff, D. M. McKay, and P. M. Sherman Conditioned Medium from Enterohemorrhagic Escherichia coli-Infected T84 Cells Inhibits Signal Transducer and Activator of Transcription 1 Activation by Gamma Interferon Infect. Immun., March 1, 2006; 74(3): 1809 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Gokhale, A. Abraham, M. A. Digman, E. Gratton, and W. Cho Phosphoinositide Specificity of and Mechanism of Lipid Domain Formation by Annexin A2-p11 Heterotetramer J. Biol. Chem., December 30, 2005; 280(52): 42831 - 42840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Riff, J. W. Callahan, and P. M. Sherman Cholesterol-Enriched Membrane Microdomains Are Required for Inducing Host Cell Cytoskeleton Rearrangements in Response to Attaching-Effacing Escherichia coli Infect. Immun., November 1, 2005; 73(11): 7113 - 7125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Markoff and V. Gerke Expression and functions of annexins in the kidney Am J Physiol Renal Physiol, November 1, 2005; 289(5): F949 - F956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang and K. R. McCrae Annexin A2 mediates endothelial cell activation by antiphospholipid/anti-{beta}2 glycoprotein I antibodies Blood, March 1, 2005; 105(5): 1964 - 1969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ma, T. Greenwell-Wild, K. Lei, W. Jin, J. Swisher, N. Hardegen, C. T. Wild, and S. M. Wahl Secretory Leukocyte Protease Inhibitor Binds to Annexin II, a Cofactor for Macrophage HIV-1 Infection J. Exp. Med., November 15, 2004; 200(10): 1337 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. G. van der Goot, G. T. van Nhieu, A. Allaoui, P. Sansonetti, and F. Lafont Rafts Can Trigger Contact-mediated Secretion of Bacterial Effectors via a Lipid-based Mechanism J. Biol. Chem., November 12, 2004; 279(46): 47792 - 47798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Conlin, S. B. Curtis, Y. Zhao, E. D. W. Moore, V. C. Smith, R. M. Meloche, B. B. Finlay, and A. M. J. Buchan Helicobacter pylori Infection Targets Adherens Junction Regulatory Proteins and Results in Increased Rates of Migration in Human Gastric Epithelial Cells Infect. Immun., September 1, 2004; 72(9): 5181 - 5192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Rescher, D. Ruhe, C. Ludwig, N. Zobiack, and V. Gerke Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranes J. Cell Sci., July 15, 2004; 117(16): 3473 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Kansau, C. Berger, M. Hospital, R. Amsellem, V. Nicolas, A. L. Servin, and M.-F. Bernet-Camard Zipper-Like Internalization of Dr-Positive Escherichia coli by Epithelial Cells Is Preceded by an Adhesin-Induced Mobilization of Raft-Associated Molecules in the Initial Step of Adhesion Infect. Immun., July 1, 2004; 72(7): 3733 - 3742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Rescher and V. Gerke Annexins - unique membrane binding proteins with diverse functions J. Cell Sci., June 1, 2004; 117(13): 2631 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Gillette and S. M. Nielsen-Preiss The role of annexin 2 in osteoblastic mineralization J. Cell Sci., January 22, 2004; 117(3): 441 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kirshner, D. Schumann, and J. E. Shively CEACAM1, a Cell-Cell Adhesion Molecule, Directly Associates with Annexin II in a Three-dimensional Model of Mammary Morphogenesis J. Biol. Chem., December 12, 2003; 278(50): 50338 - 50345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koltzscher, C. Neumann, S. Konig, and V. Gerke Ca2+-dependent Binding and Activation of Dormant Ezrin by Dimeric S100P Mol. Biol. Cell, June 1, 2003; 14(6): 2372 - 2384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. C. Harris, A. Ravandi, D. E. Awrey, and C.-H. Siu Cytoskeleton Interactions Involved in the Assembly and Function of Glycoprotein-80 Adhesion Complexes in Dictyostelium J. Biol. Chem., January 17, 2003; 278(4): 2614 - 2623. [Abstract] [Full Text] [PDF]
|
|
