QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nielsen, F. C. Articles by Christiansen, J. Search for Related Content PubMed PubMed Citation Articles by Nielsen, F. C. Articles by Christiansen, J. Social Bookmarking                         What's this?

Cytoplasmic trafficking of IGF-II mRNA-binding protein by conserved KH domains

¹ Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
² Institute of Molecular Biology, University of Copenhagen, Denmark

^* Author for correspondence (e-mail: janchr{at}mermaid.molbio.ku.dk )

Accepted 25 February 2002

The IGF-II mRNA-binding proteins (IMPs), which are composed of two RNA recognition motifs, (RRM) and four hnRNP K homology (KH) domains, have been implicated in subcytoplasmic localization of mRNAs during embryogenesis. The IMP family originated via two gene duplications before the divergence of vertebrates, and IMP homologues consisting of only the four KH motifs have been identified in Drosophila and Caenorhabditis elegans. Here we characterise the trafficking of GFP-IMP1 fusion proteins and determine the structural determinants for proper cytoplasmic localization. GFP-IMP1 is present in large 200-700 nm RNP granules, which are distributed along microtubules. In motile cells, GFP-IMP1 is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments. Granules travel in an ATP-dependent fashion at an average speed of 0.12 µm/s (range 0.04-0.22 µm/s), and cells switch from a delocalized to a localized pattern within 15-20 minutes. Both granule formation and localization are unaffected by removal of the two RRMs, whereas deletion of the KH domains, which mediate RNA-binding, impairs these functions. We conclude that IMP1 localization is associated with motility and that the major functions of IMP1 are carried out by the phylogenetically conserved KH domains.

Key words: Cytoskeleton, Microtubules, RNA localization, RNP granules, Zipcode-binding protein

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. Blumenthal and I. Ginzburg Zinc as a translation regulator in neurons: implications for P-body aggregation J. Cell Sci., October 1, 2008; 121(19): 3253 - 3260. [Abstract] [Full Text] [PDF]

				Y. Zhou, L. Rong, J. Lu, Q. Pan, and C. Liang Insulin-Like Growth Factor II mRNA Binding Protein 1 Associates with Gag Protein of Human Immunodeficiency Virus Type 1, and Its Overexpression Affects Virus Assembly J. Virol., June 15, 2008; 82(12): 5683 - 5692. [Abstract] [Full Text] [PDF]

				F. Oberman, K. Rand, Y. Maizels, A. M. Rubinstein, and J. K. Yisraeli VICKZ proteins mediate cell migration via their RNA binding activity RNA, September 1, 2007; 13(9): 1558 - 1569. [Abstract] [Full Text] [PDF]

				L. Jonson, J. Vikesaa, A. Krogh, L. K. Nielsen, T. vO. Hansen, R. Borup, A. H. Johnsen, J. Christiansen, and F. C. Nielsen Molecular Composition of IMP1 Ribonucleoprotein Granules Mol. Cell. Proteomics, May 1, 2007; 6(5): 798 - 811. [Abstract] [Full Text] [PDF]

				N. O. Knudsen, F. C. Nielsen, L. Vinther, R. Bertelsen, S. Holten-Andersen, S. E. Liberti, R. Hofstra, K. Kooi, and L. J. Rasmussen Nuclear localization of human DNA mismatch repair protein exonuclease 1 (hEXO1) Nucleic Acids Res., April 10, 2007; (2007) gkl1166v1. [Abstract] [Full Text] [PDF]

				D. Sparanese and C. H. Lee CRD-BP shields c-myc and MDR-1 RNA from endonucleolytic attack by a mammalian endoribonuclease Nucleic Acids Res., February 28, 2007; 35(4): 1209 - 1221. [Abstract] [Full Text] [PDF]

				T. Kato, S. Hayama, T. Yamabuki, N. Ishikawa, M. Miyamoto, T. Ito, E. Tsuchiya, S. Kondo, Y. Nakamura, and Y. Daigo Increased Expression of Insulin-like Growth Factor-II Messenger RNA-Binding Protein 1 Is Associated with Tumor Progression in Patients with Lung Cancer Clin. Cancer Res., January 15, 2007; 13(2): 434 - 442. [Abstract] [Full Text] [PDF]

				G. P. Patel, S. Ma, and J. Bag The autoregulatory translational control element of poly(A)-binding protein mRNA forms a heteromeric ribonucleoprotein complex Nucleic Acids Res., December 14, 2005; 33(22): 7074 - 7089. [Abstract] [Full Text] [PDF]

				N. A Hammer, T. v O Hansen, A. G. Byskov, E. Rajpert-De Meyts, M. L. Grondahl, H. E Bredkjaer, U. M Wewer, J. Christiansen, and F. C Nielsen Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer Reproduction, August 1, 2005; 130(2): 203 - 212. [Abstract] [Full Text] [PDF]

				P. Ioannidis, L. G. Mahaira, S. A. Perez, A. D. Gritzapis, P. A. Sotiropoulou, G. J. Kavalakis, A. I. Antsaklis, C. N. Baxevanis, and M. Papamichail CRD-BP/IMP1 Expression Characterizes Cord Blood CD34+ Stem Cells and Affects c-myc and IGF-II Expression in MCF-7 Cancer Cells J. Biol. Chem., May 20, 2005; 280(20): 20086 - 20093. [Abstract] [Full Text] [PDF]

				A. J. Woods, T. Kantidakis, H. Sabe, D. R. Critchley, and J. C. Norman Interaction of Paxillin with Poly(A)-Binding Protein 1 and Its Role in Focal Adhesion Turnover and Cell Migration Mol. Cell. Biol., May 1, 2005; 25(9): 3763 - 3773. [Abstract] [Full Text] [PDF]

				J. Nielsen, M. A. Kristensen, M. Willemoes, F. C. Nielsen, and J. Christiansen Sequential dimerization of human zipcode-binding protein IMP1 on RNA: a cooperative mechanism providing RNP stability Nucleic Acids Res., August 16, 2004; 32(14): 4368 - 4376. [Abstract] [Full Text] [PDF]

				T. V. O. Hansen, N. A. Hammer, J. Nielsen, M. Madsen, C. Dalbaeck, U. M. Wewer, J. Christiansen, and F. C. Nielsen Dwarfism and Impaired Gut Development in Insulin-Like Growth Factor II mRNA-Binding Protein 1-Deficient Mice Mol. Cell. Biol., May 15, 2004; 24(10): 4448 - 4464. [Abstract] [Full Text] [PDF]

				C. R. Tessier, G. A. Doyle, B. A. Clark, H. C. Pitot, and J. Ross Mammary Tumor Induction in Transgenic Mice Expressing an RNA-Binding Protein Cancer Res., January 1, 2004; 64(1): 209 - 214. [Abstract] [Full Text] [PDF]

				K. Yaniv, A. Fainsod, C. Kalcheim, and J. K. Yisraeli The RNA-binding protein Vg1 RBP is required for cell migration during early neural development Development, December 1, 2003; 130(23): 5649 - 5661. [Abstract] [Full Text] [PDF]

				K. L. Farina, S. Huttelmaier, K. Musunuru, R. Darnell, and R. H. Singer Two ZBP1 KH domains facilitate {beta}-actin mRNA localization, granule formation, and cytoskeletal attachment J. Cell Biol., January 2, 2003; 160(1): 77 - 87. [Abstract] [Full Text] [PDF]