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Journal of Cell Science 115, 2423-2431 (2002)
© 2002 The Company of Biologists Limited

Research Article

The plant Spc98p homologue colocalizes with {gamma}-tubulin at microtubule nucleation sites and is required for microtubule nucleation

Mathieu Erhardt1, Virginie Stoppin-Mellet1,*, Sarah Campagne1, Jean Canaday1, Jérôme Mutterer1, Tanja Fabian2, Margret Sauter1,2, Thierry Muller1, Christine Peter1, Anne-Marie Lambert1 and Anne-Catherine Schmit1,{ddagger}

1 Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique UPR 2357, Université Louis Pasteur, 12 rue du Général Zimmer F-67084, Strasbourg Cedex, France
2 Institut für Allgemeine Botanik, Hamburg, Ohnhorststr. 18, D-22609 Hamburg, Germany
* Present address: Laboratoire de Physiologie Cellulaire Végétale, UMR 5019 CEA/CNRS/UJF, CEA Grenoble, 17 Avenue des Martyrs 38054 Grenoble cedex 9, France

{ddagger} Author for correspondence (e-mail: anne-catherine.schmit{at}ibmp-ulp.u-strasbg.fr )

Accepted 13 March 2002

The molecular basis of microtubule nucleation is still not known in higher plant cells. This process is better understood in yeast and animals cells. In the yeast spindle pole body and the centrosome in animal cells, {gamma}-tubulin small complexes and {gamma}-tubulin ring complexes, respectively, nucleate all microtubules. In addition to {gamma}-tubulin, Spc98p or its homologues plays an essential role. We report here the characterization of rice and Arabidopsis homologues of SPC98. Spc98p colocalizes with {gamma}-tubulin at the nuclear surface where microtubules are nucleated on isolated tobacco nuclei and in living cells. AtSpc98p-GFP also localizes at the cell cortex. Spc98p is not associated with {gamma}-tubulin along microtubules. These data suggest that multiple microtubule-nucleating sites are active in plant cells. Microtubule nucleation involving Spc98p-containing {gamma}-tubulin complexes could then be conserved among all eukaryotes, despite differences in structure and spatial distribution of microtubule organizing centers.

Key words: {gamma}-TuSCs, {gamma}-TuRCs, Arabidopsis, Tobacco BY-2 cells


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