QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rodriguez-Peña, J. M. Articles by Arroyo, J. Search for Related Content PubMed PubMed Citation Articles by Rodriguez-Peña, J. M. Articles by Arroyo, J.

Mechanisms for targeting of the Saccharomyces cerevisiae GPI-anchored cell wall protein Crh2p to polarised growth sites

¹ Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
² Centro de Citometría de Flujo y Microscopía Confocal, Universidad Complutense de Madrid, 28040 Madrid, Spain

^* Author for correspondence (e-mail: jarroyo{at}farm.ucm.es )

Accepted 10 April 2002

The cell wall is an essential structure that preserves the osmotic integrity of fungal cells and determines cellular morphology during developmental programs. The high number of different wall components demands a variety of processes to deliver precursors and synthetic proteins to the proper location at the right time for wall development and modification. Here, the specificity of the mechanisms that regulate the temporal and spatial localisation of cell wall proteins to sites of polarised growth in Saccharomyces cerevisiae is investigated. For this purpose, the localisation of Crh2p, a cell wall glycosylphosphatidylinositol (GPI)-anchored mannoprotein that we have recently described as involved in cell wall construction and localised to polarised growth sites, was followed using a Crh2p-GFP fusion protein. Crh2p distribution was studied in several genetic backgrounds affected in different steps of the cell polarity establishment machinery or/and bud morphogenesis. Crh2p is localised at the mother-bud neck in bud1 cells following the random budding pattern characteristic of this mutant. The Crh2p distribution was greatly altered in a cdc42-1 mutant, indicating complete dependence on an organised actin cytoskeleton for polarised Crh2p distribution. The usual deposition of Crh2p in a ring at the base of growing buds was lacking in cdc10-11 cells growing under restrictive temperature conditions, whereas Crh2p deposition at the septum region was absent in both cdc10-11 and cdc15-lyt1 cells. These results point to the dependence of Crh2p localisation at the bud-neck on both septins and septum integrity. Furthermore, in the absence of Bni4p, a scaffold protein involved in the targeting of the chitin synthase III complex to the bud neck, Crh2p was not longer found at the neck in large-budded cells undergoing cytokinesis. Finally, Crh2p was not properly localised in cells deleted in CHS5, which encodes a protein involved in the transport of Chs3p, and was completely mislocalised in sbe2/sbe22 mutants, suggesting that the transport systems for Chs3p and Crh2p are to a certain extent coincident. The transport of other GPI-cell wall proteins, such as Cwp1p, however, does not depend on these systems as the localisation of the latter protein was not affected in either of these mutants.

Key words: Saccharomyces cerevisiae, Glycosidase, Cell wall, Crh2p, Polarity, GFP

This article has been cited by other articles:

				G. Pardini, P. W. J. De Groot, A. T. Coste, M. Karababa, F. M. Klis, C. G. de Koster, and D. Sanglard The CRH Family Coding for Cell Wall Glycosylphosphatidylinositol Proteins with a Predicted Transglycosidase Domain Affects Cell Wall Organization and Virulence of Candida albicans J. Biol. Chem., December 29, 2006; 281(52): 40399 - 40411. [Abstract] [Full Text] [PDF]

				S. Sanchatjate and R. Schekman Chs5/6 Complex: A Multiprotein Complex That Interacts with and Conveys Chitin Synthase III from the Trans-Golgi Network to the Cell Surface Mol. Biol. Cell, October 1, 2006; 17(10): 4157 - 4166. [Abstract] [Full Text] [PDF]

				Y.-W. Liu, S.-W. Lee, and F.-J. S. Lee Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane J. Cell Sci., September 15, 2006; 119(18): 3845 - 3855. [Abstract] [Full Text] [PDF]

				G. J. Smits, L. R. Schenkman, S. Brul, J. R. Pringle, and F. M. Klis Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast Mol. Biol. Cell, July 1, 2006; 17(7): 3267 - 3280. [Abstract] [Full Text] [PDF]

				G. Lesage and H. Bussey Cell Wall Assembly in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 317 - 343. [Abstract] [Full Text] [PDF]

				S. Chabane, J. Sarfati, O. Ibrahim-Granet, C. Du, C. Schmidt, I. Mouyna, M.-C. Prevost, R. Calderone, and J.-P. Latge Glycosylphosphatidylinositol-Anchored Ecm33p Influences Conidial Cell Wall Biosynthesis in Aspergillus fumigatus. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3259 - 3267. [Abstract] [Full Text] [PDF]

				T. J. Proszynski, R. W. Klemm, M. Gravert, P. P. Hsu, Y. Gloor, J. Wagner, K. Kozak, H. Grabner, K. Walzer, M. Bagnat, et al. A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast PNAS, December 13, 2005; 102(50): 17981 - 17986. [Abstract] [Full Text] [PDF]

				Q. Y. Yin, P. W. J. de Groot, H. L. Dekker, L. de Jong, F. M. Klis, and C. G. de Koster Comprehensive Proteomic Analysis of Saccharomyces cerevisiae Cell Walls: IDENTIFICATION OF PROTEINS COVALENTLY ATTACHED VIA GLYCOSYLPHOSPHATIDYLINOSITOL REMNANTS OR MILD ALKALI-SENSITIVE LINKAGES J. Biol. Chem., May 27, 2005; 280(21): 20894 - 20901. [Abstract] [Full Text] [PDF]

				M. Sanz, F. Castrejon, A. Duran, and C. Roncero Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure Microbiology, October 1, 2004; 150(10): 3229 - 3241. [Abstract] [Full Text] [PDF]

				L. Rowbottom, C. A. Munro, and N. A. R. Gow Candida albicans mutants in the BNI4 gene have reduced cell-wall chitin and alterations in morphogenesis Microbiology, October 1, 2004; 150(10): 3243 - 3252. [Abstract] [Full Text] [PDF]

				F. Gomez-Esquer, J. M. Rodriguez-Pena, G. Diaz, E. Rodriguez, P. Briza, C. Nombela, and J. Arroyo CRR1, a gene encoding a putative transglycosidase, is required for proper spore wall assembly in Saccharomyces cerevisiae Microbiology, October 1, 2004; 150(10): 3269 - 3280. [Abstract] [Full Text] [PDF]

				R. Garcia, C. Bermejo, C. Grau, R. Perez, J. M. Rodriguez-Pena, J. Francois, C. Nombela, and J. Arroyo The Global Transcriptional Response to Transient Cell Wall Damage in Saccharomyces cerevisiae and Its Regulation by the Cell Integrity Signaling Pathway J. Biol. Chem., April 9, 2004; 279(15): 15183 - 15195. [Abstract] [Full Text] [PDF]

				E. Lalanne, D. Honys, A. Johnson, G. H. H. Borner, K. S. Lilley, P. Dupree, U. Grossniklaus, and D. Twell SETH1 and SETH2, Two Components of the Glycosylphosphatidylinositol Anchor Biosynthetic Pathway, Are Required for Pollen Germination and Tube Growth in Arabidopsis PLANT CELL, January 1, 2004; 16(1): 229 - 240. [Abstract] [Full Text] [PDF]

				L. Kozubowski, H. Panek, A. Rosenthal, A. Bloecher, D. J. DeMarini, and K. Tatchell A Bni4-Glc7 Phosphatase Complex That Recruits Chitin Synthase to the Site of Bud Emergence Mol. Biol. Cell, January 1, 2003; 14(1): 26 - 39. [Abstract] [Full Text]