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doi: 10.1242/10.1242/jcs.00062

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Journal of Cell Science 115, 3837-3848 (2002)
Copyright © 2002 The Company of Biologists Limited
doi: 10.1242/jcs.00062

Research Article

Osteoclast spreading kinetics are correlated with an oscillatory activation of a calcium-dependent potassium current

Leon Espinosa, Laurent Paret, Carlos Ojeda, Yves Tourneur, Pierre D. Delmas and Chantal Chenu*

INSERM Unit 403, Hôpital E. Herriot, Pavillon F, 69437 Lyon Cedex 03, France

* Author for correspondence (chenu{at}lyon151.inserm.fr)

Accepted 24 July 2002

Cell movement and spreading involve calcium-dependent processes and ionic channel activation. During bone resorption, osteoclasts alternate between spread, motile and resorptive phases. We investigated whether the electrical membrane properties of osteoclasts were linked to their membrane morphological changes. Rabbit osteoclasts were recorded by time-lapse videomicroscopy performed simultaneously with patch-clamp whole cell and single channel recordings. Original image analysis methods were developed and used to demonstrate for the first time an oscillatory activation of a spontaneous membrane current in osteoclasts, which is directly correlated to the membrane movement rate. This current was identified as a calcium-dependent potassium current (IKCa) that is sensitive to both charybdotoxin and apamin and was generated by a channel with unitary conductance of approximately 25±2 pS. Blockade of this current also decreased osteoclast spreading and inhibited bone resorption in vitro, demonstrating a physiological role for this current in osteoclast activity. These results establish for the first time a temporal correlation between lamellipodia formation kinetics and spontaneous peaks of IKCa, which are both involved in the control of osteoclast spreading and bone resorption.

Key words: Osteoclasts, Cell movement, Potassium channels, Calcium-activated


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© The Company of Biologists Ltd 2002