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Research Article |
1 Department of Neuromuscular Diseases, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Charing Cross Campus, St Dunstan’s Road, London W6 8RP, UK
2 Randall Centre for Molecular Mechanisms of Cell Function, King’s College London, New Hunt’s House, Guy’s Campus, London SE1 1UL, UK
*Author for correspondence (e-mail: diana.watt{at}ic.ac.uk)
Accepted October 17, 2001
Normal murine dermal fibroblasts implanted into the muscles of the mdx mouse, a model for Duchenne muscular dystrophy, not only participate in new myofibre formation but also direct the expression of the protein dystrophin which is deficient in these mice. We have reported that the lectin galectin-1 is implicated in the conversion of dermal fibroblasts to muscle. In the current work we confirm the presence of galectin-1 in the medium used for conversion. Furthermore we report that exposure of clones of dermal fibroblasts to this lectin results in 100% conversion of the cells. Conversion was assessed by the expression within the cells of the muscle-specific cytoskeletal protein desmin. We also investigate the effects of galectin-1 on cells of the C2C12 mouse myogenic cell line and on primary mouse myoblasts. Exposing both transformed and primary myoblasts to the lectin resulted in an increase in fusion of cells to the terminally differentiated state in both types of cultures. Galectin-1 does not cause the myogenic conversion of murine muscle-derived fibroblasts.
Key words: Dermal fibroblast, Galectin-1, Myogenic conversion, Muscle differentiation, Myoblasts
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