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Journal of Cell Science 115, 421-431 (2002)
© 2002 The Company of Biologists Limited

Research Article

Three proteins required for early steps in the protein secretory pathway also affect nuclear envelope structure and cell cycle progression in fission yeast

Anna Matynia1,3,*, Sandra S. Salus2,3,* and Shelley Sazer1,2,3,{ddagger}

1 Department of Molecular and Cellular Biology,
2 Graduate Program in Cell and Molecular Biology,
3 Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
* These authors contributed equally to this work

{ddagger}Author for correspondence (e-mail: ssazer{at}bcm.tmc.edu)

Accepted October 18, 2001

The Ran GTPase is an essential protein that has multiple functions in eukaryotic cells. Fission yeast cells in which Ran is misregulated arrest after mitosis with condensed, unreplicated chromosomes and abnormal nuclear envelopes. The fission yeast sns mutants arrest with a similar cell cycle block and interact genetically with the Ran system. sns-A10, sns-B2 and sns-B9 have mutations in the fission yeast homologues of S. cerevisiae Sar1p, Sec31p and Sec53p, respectively, which are required for the early steps of the protein secretory pathway. The three sns mutants accumulate a normally secreted protein in the endoplasmic reticulum (ER), have an increased amount of ER membrane, and the ER/nuclear envelope lumen is dilated. Neither a post-ER block in the secretory pathway, nor ER proliferation caused by overexpression of an integral ER membrane protein, results in a cell cycle-specific defect. Therefore, the arrest seen in sns-A10, sns-B2 and sns-B9 is most likely due to nuclear envelope defects that render the cells unable to re-establish the interphase organization of the nucleus after mitosis. As a consequence, these mutants are unable to decondense their chromosomes or to initiate of the next round of DNA replication.

Key words: Protein secretion, Endoplasmic reticulum, sar1, sec31, pmm1, Phosphomannomutase, SEC53, Ran, Nuclear envelope, Cell cycle, pim1-d1, Fission yeast, S. pombe


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