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doi: 10.1242/10.1242/jcs.00085
Research Article |
1 Instituto de Microbiología Bioquímica and Departamento de
Microbiología y Genética, Consejo Superior de Investigaciones
Científicas (CSIC)/Universidad de Salamanca, 37007 Salamanca,
Spain
2 Department of Biology, Faculty of Science and Engineering, Konan University,
Okamoto 8-9-1, Kobe 658-8501, Japan
* Author for correspondence (e-mail: ribas{at}usal.es)
Accepted 1 August 2002
Schizosaccharomyces pombe Bgs1p/Cps1p has been identified as a
putative (1,3)ß-D-glucan synthase (GS) catalytic subunit with a possible
function during cytokinesis and polarized growth. To study this possibility,
double mutants of cps1-12 and cdc septation mutants were
made. The double mutants displayed several hypersensitive phenotypes and
altered actin distribution. Epistasis analysis showed mutations prior to
septum synthesis were dominant over cps1-12, while cps1-12
was dominant over the end of septation mutant cdc16-116, suggesting
Bgs1p is involved in septum cell-wall (1,3)ß-D-glucan synthesis at
cytokinesis. We have studied the in vivo physiological localization of Bgs1p
in a bgs1
strain containing a functional
GFP-bgs1+ gene (integrated single copy and expressed under
its own promoter). During vegetative growth, Bgs1p always localizes to the
growing zones: one or both ends during cell growth and contractile ring and
septum during cytokinesis. Bgs1p localization in cdc septation
mutants indicates that Bgs1p needs the medial ring and septation initiation
network (SIN) proteins to localize properly with the rest of septation
components. Bgs1p localization in the actin mutant cps8-188 shows it
depends on actin localization. In addition, Bgs1p remains polarized in the
mislocalized growing poles and septa of tea1-1 and tea2-1
mutants. During the meiotic process of the life cycle, Bgs1p localizes to the
mating projection, to the cell-to-cell contact zone during cell fusion and to
the neck area during zygote formation. Also, Bgs1p localization suggests that
it collaborates in forespore and spore wall synthesis. During spore
germination, Bgs1p localizes first around the spore during isotropic growth,
then to the zone of polarized growth and finally, to the medial ring and
septum. At the end of spore-cell division, the Bgs1p displacement to the old
end occurs only in the new cell. All these data show that Bgs1p is localized
to the areas of polarized cell wall growth and so we propose that it might be
involved in synthesizing the lineal (1,3)ß-D-glucan of the primary
septum, as well as a similar lineal (1,3)ß-D-glucan when other processes
of cell wall growth or repair are needed.
Key words: Fission yeast, Cell wall, Glucan, Septation, Polarized growth, 1,3-ß-D-glucan synthase
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