|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
doi: 10.1242/10.1242/jcs.00138
Research Article |
UMR 5546 UPS-CNRS, Pôle de Biotechnologie Végétale, 24 Chemin de Borde-Rouge, BP17, Auzeville, F-31326 Castanet-Tolosan, France
* Author for correspondence (e-mail: bottin{at}smcv.ups-tlse.fr)
Accepted 3 September 2002
The cell wall of the oomycete plant pathogen Phytophthora parasitica var. nicotianae contains a protein called CBEL that shows cellulose-binding (CB), elicitor (E) of defense in plants and lectin-like (L) activities. The biological role of this molecule in Phytophthora was investigated by generating transgenic strains suppressed in CBEL expression. Phenotypic characterization of these strains showed that they were severely impaired in adhesion to a cellophane membrane, differentiation of lobed structures in contact with cellophane, and formation of branched aggregating hyphae on cellophane and on flax cellulose fibres. Infection assays revealed that the strains suppressed in CBEL expression were not greatly affected in pathogenicity and formed branched aggregating hyphae in contact with the roots of the host plant, thereby indicating that CBEL is involved in the perception of cellulose rather than in the morphogenesis of hyphal aggregates. Interestingly, the absence of CBEL was correlated with abnormal formation of papillae-like cell wall thickenings in vitro, suggesting that CBEL is involved in cell wall deposition in Phytophthora. Reverse genetics in oomycetes has long been hampered by their diploid nature and difficulties in transformation and regeneration. The gene inactivation approach reported in this work provides the first direct evidence for intrinsic functions of an elicitor and cell wall protein in oomycetes.
Key words: Oomycete, Transformation, Silencing, Branching, Lobed structures, Cellulose, CBD
This article has been cited by other articles:
|
|
 |
|
  C. Hua, Y. Wang, X. Zheng, D. Dou, Z. Zhang, F. Govers, and Y. Wang A Phytophthora sojae G-Protein {alpha} Subunit Is Involved in Chemotaxis to Soybean Isoflavones Eukaryot. Cell, December 1, 2008; 7(12): 2133 - 2140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Savidor, R. S. Donahoo, O. Hurtado-Gonzales, M. L. Land, M. B. Shah, K. H. Lamour, and W. H. McDonald Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum Mol. Cell. Proteomics, August 1, 2008; 7(8): 1501 - 1516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Grenville-Briggs, V. L. Anderson, J. Fugelstad, A. O. Avrova, J. Bouzenzana, A. Williams, S. Wawra, S. C. Whisson, P. R.J. Birch, V. Bulone, et al. Cellulose Synthesis in Phytophthora infestans Is Required for Normal Appressorium Formation and Successful Infection of Potato PLANT CELL, March 1, 2008; 20(3): 720 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. V. Ah-Fong, Q. Xiang, and H. S. Judelson Architecture of the Sporulation-Specific Cdc14 Promoter from the Oomycete Phytophthora infestans Eukaryot. Cell, December 1, 2007; 6(12): 2222 - 2230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gaulin, N. Drame, C. Lafitte, T. Torto-Alalibo, Y. Martinez, C. Ameline-Torregrosa, M. Khatib, H. Mazarguil, F. Villalba-Mateos, S. Kamoun, et al. Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns PLANT CELL, July 1, 2006; 18(7): 1766 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Latijnhouwers and F. Govers A Phytophthora infestans G-Protein {beta} Subunit Is Involved in Sporangium Formation Eukaryot. Cell, October 1, 2003; 2(5): 971 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kamoun Molecular Genetics of Pathogenic Oomycetes Eukaryot. Cell, April 1, 2003; 2(2): 191 - 199. [Full Text] [PDF]
|
|
