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doi: 10.1242/10.1242/jcs.00150
Research Article |
1 Division of Biology, Department of Life Sciences, Graduate Program of Arts and
Sciences, University of Tokyo, Tokyo 153-8902, Japan
2 Department of Biological Sciences, Graduate School of Science, University of
Tokyo, Tokyo 113-0033, Japan
3 Department of Cell Biology, National Institute for Basic Biology, Okazaki
444-8585, Japan
* Author for correspondence (e-mail: cnakano{at}bio.c.u-tokyo.ac.jp)
Accepted 27 August 2002
We identified a novel Rho gene rho3+ and studied its interaction with diaphanous/formin for3+ in the fission yeast Schizosaccharomyces pombe. Both rho3 null cells and for3 null cells showed defects in organization of not only actin cytoskeleton but also cytoplasmic microtubules (MTs). rho3 for3 double null cells had defects that were more severe than each single null cell: polarized growth was deficient in the double null cells. Function of For3 needed the highly conserved FH1 and FH2 domains, an N-terminal region containing a Rho-binding domain, and the C-terminal region. For3 bound to active forms of both Rho3 and Cdc42 but not to that of Rho1. For3 was localized as dots to the ends of interphase cells and to the mid-region in dividing cells. This localization was probably dependent on its interaction with Rho proteins. Overexpression of For3 produced huge swollen cells containing depolarized F-actin patches and thick cytoplasmic MT bundles. In addition, overexpression of a constitutively active Rho3Q71L induced a strong defect in cytokinesis. In conclusion, we propose that the Rho3-For3 signaling system functions in the polarized cell growth of fission yeast by controlling both actin cytoskeleton and MTs.
Key words: Actin cytoskeleton, Cell polarity, Cytokinesis, Microtubule, Rho
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