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doi: 10.1242/10.1242/jcs.00168

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Journal of Cell Science 115, 4801-4808 (2002)
doi: 10.1242/jcs.00168

Research Article

Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells

Atsushi Mikami1, Sharon H. Tynan1, Taro Hama2, Katherine Luby-Phelps3, Tetsuichiro Saito2, James E. Crandall4, Joseph C. Besharse3 and Richard B. Vallee1,*

1 Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
2 Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507 Japan
3 Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA
4 Department of Developmental Neurobiology, Eunice Kennedy Shriver Center, University of Massachusetts Medical School, Waltham, MA 02452, USA

* Author for correspondence (e-mail: rv2025{at}columbia.edu)

Accepted 12 September 2002

Cytoplasmic dynein is involved in a wide variety of cellular functions. In addition to the initially characterized form (MAP 1C/dynein 1), a second form of cytoplasmic dynein (dynein 2) has been identified and implicated in intraflagellar transport (IFT) in lower eukaryotes and in Golgi organization in vertebrates. In the current study, the primary structure of the full-length dynein 2 heavy chain (HC) was determined from cDNA sequence. The dynein 1 and dynein 2 sequences were similar within the motor region, and around the light intermediate chain (LIC)-binding site within the N-terminal stem region. The dynein 2 HC co-immunoprecipitated with LIC3, a homologue of dynein 1 LICs. Dynein 2 mRNA was abundant in the ependymal layer of the neural tube and in the olfactory epithelium. Antibodies to dynein 2 HC, LIC3 and a component of IFT particles strongly stained the ependymal layer lining the lateral ventricles. Both dynein 2 HC and LIC3 staining was also observed associated with connecting cilia in the retina and within primary cilia of non-neuronal cultured cells. These data support a specific role for dynein 2 in the generation and maintenance of cilia.

Key words: Dynein, Cilium, Microtubule, Retina, Light intermediate chain, Brain




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[Abstract] [Full Text] [PDF]


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