|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Research Article |
1 Division of Biology, Department of Life Sciences, Graduate School of Arts and
Sciences, University of Tokyo, Tokyo, 153-8902, Japan
2 Department of Cell Biology, National Institute for Basic Biology, Okazaki,
444-8585, Japan
* Author for correspondence (e-mail: mabuchi{at}ims.u-tokyo.ac.jp )
Accepted 19 November 2001
Cells of the fission yeast Schizosaccharomyces pombe divide by the contraction of the F-actin ring formed at the medial region of the cell. We investigated the process of F-actin ring formation in detail using optical sectioning and three-dimensional reconstruction fluorescence microscopy. In wild-type cells, formation of an aster-like structure composed of F-actin cables and accumulation of F-actin cables were recognized at the medial cortex of the cell during prophase to metaphase. The formation of the aster-like structure seemed to initiate from branching of the longitudinal F-actin cables at a site near the spindle pole bodies, which had been duplicated but not yet separated. A single cable extended from the aster and encircled the cell at the equator to form a primary F-actin ring during metaphase. During anaphase, the accumulated F-actin cables were linked to the primary F-actin ring, and then all of these structures seemed to be packed to form the F-actin ring. These observations suggest that formation of the aster-like structure and the accumulation of the F-actin cables at the medial region of the cell during metaphase may be required to initiate the F-actin ring formation. In the nda3 mutant, which has a mutation in ß-tubulin and has been thought to be arrested at prophase, an F-actin ring with accumulated F-actin cables similar to that of anaphase wild-type cells was formed at a restrictive temperature. Immediately after shifting to a permissive temperature, this structure changed into a tightly packed ring. This suggests that the F-actin ring formation progresses beyond prophase in the nda3 cells once the cells enter prophase. We further examined F-actin structures in both cdc12 and cdc15 early cytokinesis mutants. As a result, Cdc12 seemed to be required for the primary F-actin ring formation during prophase, whereas Cdc15 may be involved in both packing the F-actin cables to form the F-actin ring and rearrangement of the F-actin after anaphase. In spg1, cdc7 and sid2 septum initiation mutants, the F-actin ring seemed to be formed in order.
Key words: F-actin ring, F-actin cable, Aster-like structure, Deconvolution, 3D reconstruction
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. T. Skau, E. M. Neidt, and D. R. Kovar Role of Tropomyosin in Formin-mediated Contractile Ring Assembly in Fission Yeast Mol. Biol. Cell, April 15, 2009; 20(8): 2160 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Roberts-Galbraith, J.-S. Chen, J. Wang, and K. L. Gould The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring J. Cell Biol., January 12, 2009; 184(1): 113 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Huang, H. Yan, and M. K. Balasubramanian Assembly of normal actomyosin rings in the absence of Mid1p and cortical nodes in fission yeast J. Cell Biol., December 15, 2008; 183(6): 979 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lammers, S. Meyer, D. Kuhlmann, and A. Wittinghofer Specificity of Interactions between mDia Isoforms and Rho Proteins J. Biol. Chem., December 12, 2008; 283(50): 35236 - 35246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Roberts-Galbraith and K. L. Gould Stepping into the ring: the SIN takes on contractile ring assembly Genes & Dev., November 15, 2008; 22(22): 3082 - 3088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Hachet and V. Simanis Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis Genes & Dev., November 15, 2008; 22(22): 3205 - 3216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Vjestica, X.-Z. Tang, and S. Oliferenko The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast Mol. Biol. Cell, March 1, 2008; 19(3): 1125 - 1138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Vavylonis, J.-Q. Wu, S. Hao, B. O'Shaughnessy, and T. D. Pollard Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast Science, January 4, 2008; 319(5859): 97 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamasaki, M. Osumi, and I. Mabuchi Three-dimensional arrangement of F-actin in the contractile ring of fission yeast J. Cell Biol., August 27, 2007; 178(5): 765 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takaine and I. Mabuchi Properties of Actin from the Fission Yeast Schizosaccharomyces pombe and Interaction with Fission Yeast Profilin J. Biol. Chem., July 27, 2007; 282(30): 21683 - 21694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Q. Wu, V. Sirotkin, D. R. Kovar, M. Lord, C. C. Beltzner, J. R. Kuhn, and T. D. Pollard Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast J. Cell Biol., July 31, 2006; 174(3): 391 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Wachtler, Y. Huang, J. Karagiannis, and M. K. Balasubramanian Cell Cycle-dependent Roles for the FCH-Domain Protein Cdc15p in Formation of the Actomyosin Ring in Schizosaccharomyces pombe Mol. Biol. Cell, July 1, 2006; 17(7): 3254 - 3266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Osumi, M. Konomi, T. Sugawara, T. Takagi, and M. Baba High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and low-voltage scanning electron microscopy and immunoelectron microscopy J. Electron Microsc. (Tokyo), April 1, 2006; 55(2): 75 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakano and I. Mabuchi Actin-depolymerizing Protein Adf1 Is Required for Formation and Maintenance of the Contractile Ring during Cytokinesis in Fission Yeast Mol. Biol. Cell, April 1, 2006; 17(4): 1933 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mutoh, K. Nakano, and I. Mabuchi Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast Genes Cells, December 1, 2005; 10(12): 1189 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gachet and J. S. Hyams Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis J. Cell Sci., September 15, 2005; 118(18): 4231 - 4242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Karagiannis, A. Bimbo, S. Rajagopalan, J. Liu, and M. K. Balasubramanian The Nuclear Kinase Lsk1p Positively Regulates the Septation Initiation Network and Promotes the Successful Completion of Cytokinesis in Response to Perturbation of the Actomyosin Ring in Schizosaccharomyces pombe Mol. Biol. Cell, January 1, 2005; 16(1): 358 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Celton-Morizur, N. Bordes, V. Fraisier, P. T. Tran, and A. Paoletti C-Terminal Anchoring of mid1p to Membranes Stabilizes Cytokinetic Ring Position in Early Mitosis in Fission Yeast Mol. Cell. Biol., December 15, 2004; 24(24): 10621 - 10635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Motegi, M. Mishra, M. K. Balasubramanian, and I. Mabuchi Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast J. Cell Biol., June 7, 2004; 165(5): 685 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Carnahan and K. L. Gould The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe J. Cell Biol., September 1, 2003; 162(5): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Evangelista, S. Zigmond, and C. Boone Formins: signaling effectors for assembly and polarization of actin filaments J. Cell Sci., July 1, 2003; 116(13): 2603 - 2611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pardo and P. Nurse Equatorial Retention of the Contractile Actin Ring by Microtubules During Cytokinesis Science, June 6, 2003; 300(5625): 1569 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Wells The youth revolution: cell biology in Japan J. Cell Biol., August 19, 2002; 158(4): 609 - 614. [Full Text] [PDF]
|
|
