spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Movie
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohashi, T.
Right arrow Articles by Erickson, H. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohashi, T.
Right arrow Articles by Erickson, H. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Cell Science 115, 1221-1229 (2002)
© 2002 The Company of Biologists Limited

Research Article

Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants

Tomoo Ohashi1, Daniel P. Kiehart2 and Harold P. Erickson1,*

1 Department of Cell Biology, Duke University Medical Center, NC27710, USA
2 Department of Biology, Duke University, NC27708, USA

* Author for correspondence (e-mail: h.erickson{at}cellbio.duke.edu )

Accepted 19 December 2001

We have prepared 3T3 cells doubly labeled to visualize simultaneously the extracellular fibronectin (FN) matrix and intracellular actin cytoskeleton in living cell cultures. We used FN-yellow fluorescent protein (FN-yfp) for the FN matrix, and the actin-binding domain of moesin fused to cyan fluorescent protein (cfp-Moe) to stain actin. Actin filament bundles were clearly seen in the protruding lamellae of the cells. FN matrix assembly appeared to be initiated as small spots of FN at the ends of actin filament bundles. The spots then elongated along the actin filament bundle toward the cell center to form FN fibrils. The end of the fibril towards the cell edge appeared immobile, and probably attached to the substrate, whereas the end toward the cell center frequently showed movements, suggesting attachment to the cell. Combining our data with the observations of Pankov et al. we suggest that fibrils grow by stretching this mobile end toward the cell center while adding new FN molecules at the end and along the entire lenght. When the cell culture was treated with cytochalasin to disrupt the actin cytoskeleton, some fibrils contracted substantially, suggesting that the segment attached primarily to the cell surface is stretched.

Key words: Fibronectin, Actin, Matrix assembly, Moesin, Green fluorescent protein


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Klotzsch, M. L. Smith, K. E. Kubow, S. Muntwyler, W. C. Little, F. Beyeler, D. Gourdon, B. J. Nelson, and V. Vogel
Fibronectin forms the most extensible biological fibers displaying switchable force-exposed cryptic binding sites
PNAS, October 27, 2009; 106(43): 18267 - 18272.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. A. Quinn, C. T. Graeber, A. R. Frackelton Jr., M. Kim, J. E. Schwarzbauer, and E. J. Filardo
Coordinate Regulation of Estrogen-Mediated Fibronectin Matrix Assembly and Epidermal Growth Factor Receptor Transactivation by the G Protein-Coupled Receptor, GPR30
Mol. Endocrinol., July 1, 2009; 23(7): 1052 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. C. Feral, A. Zijlstra, E. Tkachenko, G. Prager, M. L. Gardel, M. Slepak, and M. H. Ginsberg
CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling
J. Cell Biol., August 9, 2007; 178(4): 701 - 711.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Sivakumar, A. Czirok, B. J. Rongish, V. P. Divakara, Y.-P. Wang, and S. L. Dallas
New insights into extracellular matrix assembly and reorganization from dynamic imaging of extracellular matrix proteins in living osteoblasts
J. Cell Sci., April 1, 2006; 119(7): 1350 - 1360.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. H. Romer, K. G. Birukov, and J. G.N. Garcia
Focal Adhesions: Paradigm for a Signaling Nexus
Circ. Res., March 17, 2006; 98(5): 606 - 616.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Ohashi and H. P. Erickson
Domain Unfolding Plays a Role in Superfibronectin Formation
J. Biol. Chem., November 25, 2005; 280(47): 39143 - 39151.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. E. Robinson, R. A. Foty, and S. A. Corbett
Fibronectin Matrix Assembly Regulates {alpha}5{beta}1-mediated Cell Cohesion
Mol. Biol. Cell, March 1, 2004; 15(3): 973 - 981.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. Wierzbicka-Patynowski and J. E. Schwarzbauer
The ins and outs of fibronectin matrix assembly
J. Cell Sci., August 15, 2003; 116(16): 3269 - 3276.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2002