|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
doi: 10.1242/10.1242/jcs.00201
Research Article |
1 Department of Plant Sciences, University of Cambridge, Downing Street,
Cambridge CB2 3EA, UK
2 School of Agricultural Sciences, University of Tasmania, Hobart, Tasmania
7001, Australia
* Author for correspondence (e-mail: vd211{at}cam.ac.uk)
Accepted 1 October 2002
Free oxygen radicals are an irrefutable component of life, underlying important biochemical and physiological phenomena in animals. Here it is shown that free oxygen radicals activate plasma membrane Ca2+- and K+-permeable conductances in Arabidopsis root cell protoplasts, mediating Ca2+ influx and K+ efflux, respectively. Free oxygen radicals generate increases in cytosolic Ca2+ mediated by a novel population of nonselective cation channels that differ in selectivity and pharmacology from those involved in toxic Na+ influx. Analysis of the free oxygen radical-activated K+ conductance showed its similarity to the Arabidopsis root K+ outward rectifier. Significantly larger channel activation was found in cells responsible for perceiving environmental signals and undergoing elongation. Quenching root free oxygen radicals inhibited root elongation, confirming the role of radical-activated Ca2+ influx in cell growth. Net free oxygen radical-stimulated Ca2+ influx and K+ efflux were observed in root cells of monocots, dicots, C3 and C4 plants, suggesting conserved mechanisms and functions. In conclusion, two functions for free oxygen radical cation channel activation are proposed: initialization/amplification of stress signals and control of cell elongation in root growth.
Key words: Arabidopsis, Calcium, Channel, Free oxygen radical, Plasma membrane, Potassium
This article has been cited by other articles:
|
|
 |
|
  Z. Chen, T. A. Cuin, M. Zhou, A. Twomey, B. P. Naidu, and S. Shabala Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance J. Exp. Bot., December 1, 2007; 58(15-16): 4245 - 4255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Jones, M. J. Raymond, Z. Yang, and N. Smirnoff NADPH oxidase-dependent reactive oxygen species formation required for root hair growth depends on ROP GTPase J. Exp. Bot., April 1, 2007; 58(6): 1261 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Kwak, V. Nguyen, and J. I. Schroeder The Role of Reactive Oxygen Species in Hormonal Responses Plant Physiology, June 1, 2006; 141(2): 323 - 329. [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shin, R. H. Berg, and D. P. Schachtman Reactive Oxygen Species and Root Hairs in Arabidopsis Root Response to Nitrogen, Phosphorus and Potassium Deficiency Plant Cell Physiol., August 1, 2005; 46(8): 1350 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Tegg, L. Melian, C. R. Wilson, and S. Shabala Plant Cell Growth and Ion Flux Responses to the Streptomycete Phytotoxin Thaxtomin A: Calcium and Hydrogen Flux Patterns Revealed by the Non-invasive MIFE Technique Plant Cell Physiol., April 1, 2005; 46(4): 638 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Chen, R. Huang, Y.-M. Xiao, P. Lu, J. Chen, and X.-C. Wang Extracellular Calmodulin-Induced Stomatal Closure Is Mediated by Heterotrimeric G Protein and H2O2 Plant Physiology, December 1, 2004; 136(4): 4096 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Armengaud, R. Breitling, and A. Amtmann The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling Plant Physiology, September 1, 2004; 136(1): 2556 - 2576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ludidi, M. Morse, M. Sayed, T. Wherrett, S. Shabala, and C. Gehring A Recombinant Plant Natriuretic Peptide Causes Rapid and Spatially Differentiated K+, Na+ and H+ Flux Changes in Arabidopsis thaliana Roots Plant Cell Physiol., August 15, 2004; 45(8): 1093 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Rentel and M. R. Knight Oxidative Stress-Induced Calcium Signaling in Arabidopsis Plant Physiology, July 1, 2004; 135(3): 1471 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shin and D. P. Schachtman Hydrogen peroxide mediates plant root cell response to nutrient deprivation PNAS, June 8, 2004; 101(23): 8827 - 8832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Rodriguez, A. R. Cordoba, L. Ortega, and E. Taleisnik Decreased reactive oxygen species concentration in the elongation zone contributes to the reduction in maize leaf growth under salinity J. Exp. Bot., June 1, 2004; 55(401): 1383 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Mori and J. I. Schroeder Reactive Oxygen Species Activation of Plant Ca2+ Channels. A Signaling Mechanism in Polar Growth, Hormone Transduction, Stress Signaling, and Hypothetically Mechanotransduction Plant Physiology, June 1, 2004; 135(2): 702 - 708. [Full Text] [PDF]
|
|
|
|
|
|
E. B. Blancaflor and P. H. Masson Plant Gravitropism. Unraveling the Ups and Downs of a Complex Process Plant Physiology, December 1, 2003; 133(4): 1677 - 1690. [Full Text] [PDF]
|
|
|
|
 |
|
 S. SHABALA Regulation of Potassium Transport in Leaves: from Molecular to Tissue Level Ann. Bot., November 1, 2003; 92(5): 627 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Demidchik, C. Nichols, M. Oliynyk, A. Dark, B. J. Glover, and J. M. Davies Is ATP a Signaling Agent in Plants? Plant Physiology, October 1, 2003; 133(2): 456 - 461. [Full Text] [PDF]
|
|
|
|
|
|
P. A. Essah, R. Davenport, and M. Tester Sodium Influx and Accumulation in Arabidopsis Plant Physiology, September 1, 2003; 133(1): 307 - 318. [Abstract] [Full Text] [PDF]
|
|
