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First published online 1 April 2003
doi: 10.1242/jcs.00420
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Research Article |
,
Center for the Study of Nervous System Injury and Department of
Neurology, Washington University School of Medicine, St Louis, MO 63110,
USA
* Merck Research Labs, West Point, PA 19486, USA
Department of Pharmaceutical Sciences, School of Pharmacy, Medical University
of South Carolina, 280 Calhoun Street, Charleston, SC 29425 USA
Author for correspondence (e-mail:
yusp{at}musc.edu)
Accepted 29 January 2003
The Na+, K+-ATPase (Na+, K+-pump) plays critical roles in maintaining ion homeostasis. Blocking the Na+, K+-pump may lead to apoptosis. By contrast, whether an apoptotic insult may affect the Na+, K+-pump activity is largely undefined. In cultured cortical neurons, the Na+, K+-pump activity measured as a membrane current Ipump was time-dependently suppressed by apoptotic insults including serum deprivation, staurosporine, and C2-ceramide, concomitant with depletion of intracellular ATP and production of reactive oxygen species. Signifying a putative relationship among these events, Ipump was highly sensitive to changes in ATP and reactive oxygen species levels. Moreover, the apoptosis-associated Na+, K+-pump failure and serum deprivation-induced neuronal death were antagonized by pyruvate and succinate in ATP- and reactive-oxygen-species-dependent manners. We suggest that failure of the Na+, K+-pump as a result of a combination of energy deficiency and production of reactive oxygen species is a common event in the apoptotic cascade; preserving the pump activity provides a neuroprotective strategy in certain pathological conditions.
Key words: Na+, K+-ATPase, Apoptosis, Potassium homeostasis, Neuron
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