|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 30 April 2003
doi: 10.1242/jcs.00464
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Swiss Federal Institute of Technology (ETH) Zürich, Institute of Biochemistry, HPM F11.1, CH-8093 Zürich, Switzerland
* Author for correspondence (e-mail: ulrike.kutay{at}bc.biol.ethz.ch)
Accepted 7 March 2003
The production of ribosomes constitutes a major biosynthetic task for cells. Eukaryotic small and large ribosomal subunits are assembled in the nucleolus and independently exported to the cytoplasm. Most nuclear export pathways require RanGTP-binding export receptors. We analyzed the role of CRM1, the export receptor for leucine-rich nuclear export signals (NES), in the biogenesis of ribosomal subunits in vertebrate cells. Inhibition of the CRM1 export pathway led to a defect in nuclear export of both 40S and 60S subunits in HeLa cells. Moreover, the export of newly made ribosomal subunits in Xenopus oocytes was efficiently and specifically competed by BSA-NES conjugates. The CRM1 dependence of 60S subunit export suggested a conserved function for NMD3, a factor proposed to be a 60S subunit export adaptor in yeast. Indeed, we observed that nuclear export of human NMD3 (hNMD3) is sensitive to leptomycin B (LMB), which inactivates CRM1. It had, however, not yet been demonstrated that Nmd3 can interact with CRM1. Using purified recombinant proteins we have shown here that hNMD3 binds to CRM1 directly, in a RanGTP-dependent manner, by way of a C-terminal NES sequence. Our results suggest that the functions of CRM1 and NMD3 in ribosomal subunit export are conserved from yeast to higher eukaryotes.
Key words: Nucleo-cytoplasmic transport, Ribosome, Export, CRM1, NMD3, LMB
This article has been cited by other articles:
|
|
 |
|
  D. Engelsma, N. Valle, A. Fish, N. Salome, J. M. Almendral, and M. Fornerod A Supraphysiological Nuclear Export Signal Is Required for Parvovirus Nuclear Export Mol. Biol. Cell, June 1, 2008; 19(6): 2544 - 2552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. White, Z. Li, R. Sardana, J. M. Bujnicki, E. M. Marcotte, and A. W. Johnson Bud23 Methylates G1575 of 18S rRNA and Is Required for Efficient Nuclear Export of Pre-40S Subunits Mol. Cell. Biol., May 15, 2008; 28(10): 3151 - 3161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-J. Hung, K.-Y. Lo, S. S. Patel, K. Helmke, and A. W. Johnson Arx1 Is a Nuclear Export Receptor for the 60S Ribosomal Subunit in Yeast Mol. Biol. Cell, February 1, 2008; 19(2): 735 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Atanasiu and N. W. Fraser The Stable 2-Kilobase Latency-Associated Transcript of Herpes Simplex Virus Type 1 Can Alter the Assembly of the 60S Ribosomal Subunit and Is Exported from Nucleus to Cytoplasm by a CRM1-Dependent Pathway J. Virol., July 15, 2007; 81(14): 7695 - 7701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. West, J. B. Hedges, K.-Y. Lo, and A. W. Johnson Novel Interaction of the 60S Ribosomal Subunit Export Adapter Nmd3 at the Nuclear Pore Complex J. Biol. Chem., May 11, 2007; 282(19): 14028 - 14037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bayfield, T. E. Kaiser, R. V. Intine, and R. J. Maraia Conservation of a Masked Nuclear Export Activity of La Proteins and Its Effects on tRNA Maturation Mol. Cell. Biol., May 1, 2007; 27(9): 3303 - 3312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hedges, Y.-I Chen, M. West, C. Bussiere, and A. W. Johnson Mapping the Functional Domains of Yeast NMD3, the Nuclear Export Adapter for the 60 S Ribosomal Subunit J. Biol. Chem., December 1, 2006; 281(48): 36579 - 36587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Seiser, A. E. Sundberg, B. J. Wollam, P. Zobel-Thropp, K. Baldwin, M. D. Spector, and D. E. Lycan Ltv1 Is Required for Efficient Nuclear Export of the Ribosomal Small Subunit in Saccharomyces cerevisiae Genetics, October 1, 2006; 174(2): 679 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bernad, D. Engelsma, H. Sanderson, H. Pickersgill, and M. Fornerod Nup214-Nup88 Nucleoporin Subcomplex Is Required for CRM1-mediated 60 S Preribosomal Nuclear Export J. Biol. Chem., July 14, 2006; 281(28): 19378 - 19386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-J. Hung and A. W. Johnson Nuclear Recycling of the Pre-60S Ribosomal Subunit-Associated Factor Arx1 Depends on Rei1 in Saccharomyces cerevisiae Mol. Cell. Biol., May 15, 2006; 26(10): 3718 - 3727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Z. Scheifele, E. P. Ryan, and L. J. Parent Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein J. Virol., July 15, 2005; 79(14): 8732 - 8741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. West, J. B. Hedges, A. Chen, and A. W. Johnson Defining the Order in Which Nmd3p and Rpl10p Load onto Nascent 60S Ribosomal Subunits Mol. Cell. Biol., May 1, 2005; 25(9): 3802 - 3813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Daelemans, S. V. Costes, S. Lockett, and G. N. Pavlakis Kinetic and Molecular Analysis of Nuclear Export Factor CRM1 Association with Its Cargo In Vivo Mol. Cell. Biol., January 15, 2005; 25(2): 728 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Oeffinger, M. Dlakic, and D. Tollervey A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits Genes & Dev., January 15, 2004; 18(2): 196 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Ritland Politz, R. A. Tuft, and T. Pederson Diffusion-based Transport of Nascent Ribosomes in the Nucleus Mol. Biol. Cell, December 1, 2003; 14(12): 4805 - 4812. [Abstract] [Full Text] [PDF]
|
|
