QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 6 May 2003
doi: 10.1242/jcs.00453

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.00453v1 116/12/2431    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Trelles-Sticken, E. Articles by Scherthan, H. Search for Related Content PubMed PubMed Citation Articles by Trelles-Sticken, E. Articles by Scherthan, H.

Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres

¹ Max-Planck-Institute for Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany
² Institute of Botany, University of Vienna, Vienna, Austria

^* Author for correspondence (e-mail: schertha{at}molgen.mpg.de)

Accepted 4 March 2003

We investigated the sequence of chromosomal events during meiotic prophase in haploid, diploid and autotetraploid SK1 strains of Saccharomyces cerevisiae. Using molecular cytology, we found that meiosis-specific nuclear topology (i.e. dissolution of centromere clustering, bouquet formation and meiotic divisions) are significantly delayed in polyploid SK1 meiosis. Thus, and in contrast to the situation in plants, an increase in ploidy extends prophase I in budding yeast. Moreover, we found that bouquet formation also occurs in haploid and diploid SK1 meiosis deficient in the telomeric heterochromatin protein Sir3p. Diploid sir3 SK1 meiosis showed pleiotropic defects such as delayed centromere cluster resolution in a proportion of cells and impeded downstream events (i.e. bouquet formation, homologue pairing and meiotic divisions). Meiotic telomere clustering occurred in diploid and haploid sir3 strains. Using the haploid system, we further show that a bouquet forms at the kar3 SPB. Comparison of the expression of meiosis-specific Ndj1p-HA and Zip1p in haploid control and kar3 time courses revealed that fewer cells enter the meiotic cycle in absence of Kar3p. Elevated frequencies of bouquets in kar3 haploid meiosis suggest a role for Kar3p in regulation of telomere dynamics.

Key words: Bouquet, Haploid, Kar3, Meiosis, Polyploid, Sir3, Telomeres, Yeast

This article has been cited by other articles:

				H.-Y. Wu and S. M. Burgess Ndj1, a Telomere-Associated Protein, Promotes Meiotic Recombination in Budding Yeast Mol. Cell. Biol., May 15, 2006; 26(10): 3683 - 3694. [Abstract] [Full Text] [PDF]

				E. Trelles-Sticken, S. Bonfils, J. Sollier, V. Geli, H. Scherthan, and C. de La Roche Saint-Andre Set1- and Clb5-deficiencies disclose the differential regulation of centromere and telomere dynamics in Saccharomyces cerevisiae meiosis J. Cell Sci., November 1, 2005; 118(21): 4985 - 4994. [Abstract] [Full Text] [PDF]

				S. L. Page and R. S. Hawley The Drosophila Meiotic Mutant mei-352 Is an Allele of klp3A and Reveals a Role for a Kinesin-like Protein in Crossover Distribution Genetics, August 1, 2005; 170(4): 1797 - 1807. [Abstract] [Full Text] [PDF]

				E. Trelles-Sticken, C. Adelfalk, J. Loidl, and H. Scherthan Meiotic telomere clustering requires actin for its formation and cohesin for its resolution J. Cell Biol., July 18, 2005; 170(2): 213 - 223. [Abstract] [Full Text] [PDF]