QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 6 May 2003
doi: 10.1242/jcs.00465

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.00465v1 116/12/2531    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wojcikiewicz, E. P. Articles by Moy, V. T. Search for Related Content PubMed PubMed Citation Articles by Wojcikiewicz, E. P. Articles by Moy, V. T.

Contributions of molecular binding events and cellular compliance to the modulation of leukocyte adhesion

Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, FL 33136, USA

^* Author for correspondence (e-mail: vmoy{at}miami.edu)

Accepted 7 March 2003

The interaction of leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) is central to the regulation of adhesion in leukocytes. In this report, we investigated the mechanisms by which phorbol myristate acetate (PMA) promotes LFA-1-dependent cell adhesion. The adhesion of PMA-stimulated cells to immobilized ICAM-1 was quantified in direct force measurements acquired by atomic force microscopy (AFM). Enhanced adhesion of PMA-stimulated cells to immobilized ICAM-1 stemmed from an increase in the number of LFA-1–ICAM-1 complexes formed between the two apposing surfaces on contact, rather than by affinity modulation of LFA-1. Single molecule force measurements revealed that the force spectrum of the LFA-1–ICAM-1 complex formed by PMA-stimulated cells is identical to the force spectrum of the complex formed by resting cells. Thus, PMA stimulation does not modify the mechanical strength of the individual LFA-1–ICAM-1 interaction. Instead, the enhanced cell adhesion of PMA-stimulated cells appears to be a complex process that correlates with changes in the mechanical properties of the cell. We estimate that changes in the elasticity of the cell gave rise to a more than 10-fold increase in cell adhesion.

Key words: Cell adhesion, Cell compliance, Integrins, Leukocyte, AFM, Single molecule measurements

This article has been cited by other articles:

				A. Rabodzey, P. Alcaide, F. W. Luscinskas, and B. Ladoux Mechanical Forces Induced by the Transendothelial Migration of Human Neutrophils Biophys. J., August 1, 2008; 95(3): 1428 - 1438. [Abstract] [Full Text] [PDF]

				J. te Riet, A. W. Zimmerman, A. Cambi, B. Joosten, S. Speller, R. Torensma, F. N. van Leeuwen, C. G. Figdor, and F. de Lange Distinct kinetic and mechanical properties govern ALCAM-mediated interactions as shown by single-molecule force spectroscopy J. Cell Sci., November 15, 2007; 120(22): 3965 - 3976. [Abstract] [Full Text] [PDF]

				P. Roca-Cusachs, I. Almendros, R. Sunyer, N. Gavara, R. Farre, and D. Navajas Rheology of Passive and Adhesion-Activated Neutrophils Probed by Atomic Force Microscopy Biophys. J., November 1, 2006; 91(9): 3508 - 3518. [Abstract] [Full Text] [PDF]

				X. Zhang, E. P. Wojcikiewicz, and V. T. Moy Dynamic Adhesion of T Lymphocytes to Endothelial Cells Revealed by Atomic Force Microscopy Experimental Biology and Medicine, September 1, 2006; 231(8): 1306 - 1312. [Abstract] [Full Text] [PDF]

				M. J. Rosenbluth, W. A. Lam, and D. A. Fletcher Force Microscopy of Nonadherent Cells: A Comparison of Leukemia Cell Deformability Biophys. J., April 15, 2006; 90(8): 2994 - 3003. [Abstract] [Full Text] [PDF]

				S. Sen, S. Subramanian, and D. E. Discher Indentation and Adhesive Probing of a Cell Membrane with AFM: Theoretical Model and Experiments Biophys. J., November 1, 2005; 89(5): 3203 - 3213. [Abstract] [Full Text] [PDF]

				X. Zhang, S. E. Craig, H. Kirby, M. J. Humphries, and V. T. Moy Molecular Basis for the Dynamic Strength of the Integrin {alpha}4{beta}1/VCAM-1 Interaction Biophys. J., November 1, 2004; 87(5): 3470 - 3478. [Abstract] [Full Text] [PDF]

				W. D. Hanley, D. Wirtz, and K. Konstantopoulos Distinct kinetic and mechanical properties govern selectin-leukocyte interactions J. Cell Sci., May 15, 2004; 117(12): 2503 - 2511. [Abstract] [Full Text] [PDF]

				X. Zhang, A. Chen, D. De Leon, H. Li, E. Noiri, V. T. Moy, and M. S. Goligorsky Atomic force microscopy measurement of leukocyte-endothelial interaction Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H359 - H367. [Abstract] [Full Text] [PDF]