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First published online 13 May 2003
doi: 10.1242/jcs.00476

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Journal of Cell Science 116, 2647-2654 (2003)
doi: 10.1242/jcs.00476

Research Article

Unexpected roles of a Dictyostelium homologue of eukaryotic EF-2 in growth and differentiation

Sohsuke Watanabe, Kohji Sakurai, Aiko Amagai and Yasuo Maeda*

Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8578, Japan

* Author for correspondence (e-mail: ymaeda{at}mail.cc.tohoku.ac.jp)

Accepted 13 March 2003

EF-2 is believed to be indispensable for polypeptide chain elongation in protein synthesis and therefore for cell proliferation. Surprisingly, we could isolate ef2 null cells from Dictyostelium discoideum that exhibited almost normal growth and protein synthesis, which suggests that there is another molecule capable of compensating for EF-2 function. The knock-out of Dictyostelium EF-2 (Dd-EF2H; 101 kDa phosphoprotein) impairs cytokinesis, resulting in formation of multinucleate cells. The initiation of differentiation, including the acquisition of aggregation competence, was delayed in Dd-ef2 null cells compared with that in wild-type. By contrast, Dd-ef2 overexpression enhanced the progression of differentiation, thus indicating a positive involvement of Dd-EF2H in growth/differentiation transition.

Key words: Dictyostelium discoideum, EF-2, Cell cycle, Growth, Differentiation, Cytokinesis, Mitochondria


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