|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online July 10, 2003
doi: 10.1242/10.1242/jcs.00670
Commentary |
Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014 USA
* Author for correspondence (e-mail: jschwarzbauer{at}molbio.princeton.edu)
Cell phenotype is specified by environmental cues embedded in the architecture and composition of the extracellular matrix (ECM). Much has been learned about matrix organization and assembly through analyses of the ECM protein fibronectin (FN). FN matrix assembly is a cell-mediated process in which soluble dimeric FN is converted into a fibrillar network. Binding of cell surface integrin receptors to FN converts it to an active form, which promotes fibril formation through interactions with other cell-associated FN dimers. As FN fibrils form on the outside of the cell, cytoplasmic domains of integrin receptors organize cytoplasmic proteins into functional complexes inside. Intracellular connections to the actin cytoskeletal network and stimulation of certain key intracellular signaling pathways are essential for FN–integrin interactions and propagation of FN fibril formation. Thus, assembly of native functional ECM depends on exquisite coordination between extracellular events and intracellular pathways.
Key words: Extracellular matrix, Fibronectin, Integrin, Fibril, Receptor, Intracellular signaling
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  A. Corti, F. Curnis, W. Arap, and R. Pasqualini The neovasculature homing motif NGR: more than meets the eye Blood, October 1, 2008; 112(7): 2628 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Feijoo-Cuaresma, F. Mendez, A. Maqueda, M. A. Esteban, S. Naranjo-Suarez, M. C. Castellanos, M. H. del Cerro, S. N. Vazquez, A. Garcia-Pardo, M. O. Landazuri, et al. Inadequate Activation of the GTPase RhoA Contributes to the Lack of Fibronectin Matrix Assembly in von Hippel-Lindau Protein-defective Renal Cancer Cells J. Biol. Chem., September 5, 2008; 283(36): 24982 - 24990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kinsey, M. R. Williamson, S. Chaudhry, K. T. Mellody, A. McGovern, S. Takahashi, C. A. Shuttleworth, and C. M. Kielty Fibrillin-1 microfibril deposition is dependent on fibronectin assembly J. Cell Sci., August 15, 2008; 121(16): 2696 - 2704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huveneers, H. Truong, R. Fassler, A. Sonnenberg, and E. H. J. Danen Binding of soluble fibronectin to integrin {alpha}5{beta}1 - link to focal adhesion redistribution and contractile shape J. Cell Sci., August 1, 2008; 121(15): 2452 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Park, C. Will, B. Martin, L. Gullotti, N. Friedrichs, R. Buettner, H. Schneider, S. Ludwig, and V. Wixler Deficiency in the LIM-only protein FHL2 impairs assembly of extracellular matrix proteins FASEB J, July 1, 2008; 22(7): 2508 - 2520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vial and P. J. McKeown-Longo PAI1 stimulates assembly of the fibronectin matrix in osteosarcoma cells through crosstalk between the {alpha}v{beta}5 and {alpha}5{beta}1 integrins J. Cell Sci., May 15, 2008; 121(10): 1661 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schlunck, H. Han, T. Wecker, D. Kampik, T. Meyer-ter-Vehn, and F. Grehn Substrate Rigidity Modulates Cell Matrix Interactions and Protein Expression in Human Trabecular Meshwork Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 262 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Tomkowicz, K. Rybinski, B. Foley, W. Ebel, B. Kline, E. Routhier, P. Sass, N. C. Nicolaides, L. Grasso, and Y. Zhou Interaction of endosialin/TEM1 with extracellular matrix proteins mediates cell adhesion and migration PNAS, November 13, 2007; 104(46): 17965 - 17970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Shynlova, S. J. Williams, H. Draper, B. G White, D. J MacPhee, and S. J Lye Uterine Stretch Regulates Temporal and Spatial Expression of Fibronectin Protein and Its Alpha 5 Integrin Receptor in Myometrium of Unilaterally Pregnant Rats Biol Reprod, November 1, 2007; 77(5): 880 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takahashi, M. Leiss, M. Moser, T. Ohashi, T. Kitao, D. Heckmann, A. Pfeifer, H. Kessler, J. Takagi, H. P. Erickson, et al. The RGD motif in fibronectin is essential for development but dispensable for fibril assembly J. Cell Biol., October 3, 2007; 178(1): 167 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rifes, L. Carvalho, C. Lopes, R. P. Andrade, G. Rodrigues, I. Palmeirim, and S. Thorsteinsdottir Redefining the role of ectoderm in somitogenesis: a player in the formation of the fibronectin matrix of presomitic mesoderm Development, September 1, 2007; 134(17): 3155 - 3165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Feral, A. Zijlstra, E. Tkachenko, G. Prager, M. L. Gardel, M. Slepak, and M. H. Ginsberg CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling J. Cell Biol., August 9, 2007; 178(4): 701 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. He, G. Prasanna, and T. Yorio Endothelin-1-Mediated Signaling in the Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Astrocytes Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3737 - 3745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A-H. Kwon, Z. Qiu, and Y. Hirao Topical Application of Plasma Fibronectin in Full-Thickness Skin Wound Healing in Rats Experimental Biology and Medicine, July 1, 2007; 232(7): 935 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Przybysz, K. Borysewicz, J. Szechinski, and I. Katnik-Prastowska Synovial fibronectin fragmentation and domain expressions in relation to rheumatoid arthritis progression Rheumatology, July 1, 2007; 46(7): 1071 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Marom, M. A. Rahat, N. Lahat, L. Weiss-Cerem, A. Kinarty, and H. Bitterman Native and fragmented fibronectin oppositely modulate monocyte secretion of MMP-9 J. Leukoc. Biol., June 1, 2007; 81(6): 1466 - 1476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Papp, M. P. Fadel, H. Kim, C. A. McCulloch, and M. Opas Calreticulin Affects Fibronectin-based Cell-Substratum Adhesion via the Regulation of c-Src Activity J. Biol. Chem., June 1, 2007; 282(22): 16585 - 16598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tura, S. Grisanti, K. Petermeier, and S. Henke-Fahle The Rho-Kinase Inhibitor H-1152P Suppresses the Wound-Healing Activities of Human Tenon's Capsule Fibroblasts In Vitro Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2152 - 2161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Knoblauch, C. Will, G. Goncharenko, S. Ludwig, and V. Wixler The binding of Mss4 to {alpha}-integrin subunits regulates matrix metalloproteinase activation and fibronectin remodeling FASEB J, February 1, 2007; 21(2): 497 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Moore, X.-D. Shen, F. Gao, R. W. Busuttil, and A. J. Coito Fibronectin-{alpha}4{beta}1 Integrin Interactions Regulate Metalloproteinase-9 Expression in Steatotic Liver Ischemia and Reperfusion Injury Am. J. Pathol., February 1, 2007; 170(2): 567 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zaidel-Bar, R. Milo, Z. Kam, and B. Geiger A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions J. Cell Sci., January 1, 2007; 120(1): 137 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Sher, C.-C. Chou, R.-H. Chou, H.-M. Wu, W.-S. Wayne Chang, C.-H. Chen, P.-C. Yang, C.-W. Wu, C.-L. Yu, and K. Peck Human Kallikrein 8 Protease Confers a Favorable Clinical Outcome in Non-Small Cell Lung Cancer by Suppressing Tumor Cell Invasiveness Cancer Res., December 15, 2006; 66(24): 11763 - 11770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. U. Sehgal, P. J. DeBiase, S. Matzno, T.-L. Chew, J. N. Claiborne, S. B. Hopkinson, A. Russell, M. P. Marinkovich, and J. C. R. Jones Integrin beta4 Regulates Migratory Behavior of Keratinocytes by Determining Laminin-332 Organization J. Biol. Chem., November 17, 2006; 281(46): 35487 - 35498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gui, K. Wojciechowski, C. D. Gildner, H. Nedelkovska, and D. C. Hocking Identification of the Heparin-binding Determinants within Fibronectin Repeat III1: ROLE IN CELL SPREADING AND GROWTH J. Biol. Chem., November 17, 2006; 281(46): 34816 - 34825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. de Laplanche, K. Gouget, G. Cleris, F. Dragounoff, J. Demont, A. Morales, L. Bezin, C. Godinot, G. Perriere, D. Mouchiroud, et al. Physiological oxygenation status is required for fully differentiated phenotype in kidney cortex proximal tubules Am J Physiol Renal Physiol, October 1, 2006; 291(4): F750 - F760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dong, J. M. Muriel, S. Ramirez, H. Hutter, E. M. Hedgecock, L. Breydo, I. V. Baskakov, and B. E. Vogel Hemicentin Assembly in the Extracellular Matrix Is Mediated by Distinct Structural Modules J. Biol. Chem., August 18, 2006; 281(33): 23606 - 23610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Winters, B. K. M. Raj, E. E. Robinson, R. A. Foty, and S. A. Corbett Three-dimensional Culture Regulates Raf-1 Expression to Modulate Fibronectin Matrix Assembly Mol. Biol. Cell, August 1, 2006; 17(8): 3386 - 3396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. DeBiase, K. Lane, S. Budinger, K. Ridge, M. Wilson, and J. C.R. Jones Laminin-311 (Laminin-6) Fiber Assembly by Type I-like Alveolar Cells J. Histochem. Cytochem., June 1, 2006; 54(6): 665 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Wiita, S. R. K. Ainavarapu, H. H. Huang, and J. M. Fernandez From the Cover: Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques PNAS, May 9, 2006; 103(19): 7222 - 7227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cho and D. F. Mosher Enhancement of thrombogenesis by plasma fibronectin cross-linked to fibrin and assembled in platelet thrombi Blood, May 1, 2006; 107(9): 3555 - 3563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lagana, J. G. Goetz, P. Cheung, A. Raz, J. W. Dennis, and I. R. Nabi Galectin Binding to Mgat5-Modified N-Glycans Regulates Fibronectin Matrix Remodeling in Tumor Cells Mol. Cell. Biol., April 15, 2006; 26(8): 3181 - 3193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Monaghan-Benson and P. J. McKeown-Longo Urokinase-type Plasminogen Activator Receptor Regulates a Novel Pathway of Fibronectin Matrix Assembly Requiring Src-dependent Transactivation of Epidermal Growth Factor Receptor J. Biol. Chem., April 7, 2006; 281(14): 9450 - 9459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H.L. Hamelers, C. Olivo, A. E.E. Mertens, D. M. Pegtel, R. A. van der Kammen, A. Sonnenberg, and J. G. Collard The Rac activator Tiam1 is required for {alpha}3{beta}1-mediated laminin-5 deposition, cell spreading, and cell migration J. Cell Biol., December 5, 2005; 171(5): 871 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mao and J. E. Schwarzbauer Stimulatory effects of a three-dimensional microenvironment on cell-mediated fibronectin fibrillogenesis J. Cell Sci., October 1, 2005; 118(19): 4427 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Jaeger, A. K. Spiegel, N. H. Ing, G. A. Johnson, F. W. Bazer, and R. C. Burghardt Functional Effects of Transforming Growth Factor {beta} on Adhesive Properties of Porcine Trophectoderm Endocrinology, September 1, 2005; 146(9): 3933 - 3942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wang, R. A. F. Clark, D. F. Mosher, and X.-D. Ren Fibronectin's Central Cell-binding Domain Supports Focal Adhesion Formation and Rho Signal Transduction J. Biol. Chem., August 5, 2005; 280(31): 28803 - 28810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fogelgren, N. Polgar, K. M. Szauter, Z. Ujfaludi, R. Laczko, K. S. K. Fong, and K. Csiszar Cellular Fibronectin Binds to Lysyl Oxidase with High Affinity and Is Critical for Its Proteolytic Activation J. Biol. Chem., July 1, 2005; 280(26): 24690 - 24697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Tang, R.-S. Yang, and W.-M. Fu Prostaglandin E2 Stimulates Fibronectin Expression through EP1 Receptor, Phospholipase C, Protein Kinase C{alpha}, and c-Src Pathway in Primary Cultured Rat Osteoblasts J. Biol. Chem., June 17, 2005; 280(24): 22907 - 22916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. H. Jarvinen, T. L. N. Jarvinen, M. Kaariainen, H. Kalimo, and M. Jarvinen Muscle Injuries: Biology and Treatment Am. J. Sports Med., May 1, 2005; 33(5): 745 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.J. Williams, B.G. White, and D.J. MacPhee Expression of {alpha}5 Integrin (Itga5) Is Elevated in the Rat Myometrium During Late Pregnancy and Labor: Implications for Development of a Mechanical Syncytium Biol Reprod, May 1, 2005; 72(5): 1114 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Canty and K. E. Kadler Procollagen trafficking, processing and fibrillogenesis J. Cell Sci., April 1, 2005; 118(7): 1341 - 1353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Shanker, K. Yamada, K. G. Green, K. A. Yamada, and J. E. Saffitz Matrix Protein-Specific Regulation of Cx43 Expression in Cardiac Myocytes Subjected to Mechanical Load Circ. Res., March 18, 2005; 96(5): 558 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ambesi, R. M. Klein, K. M. Pumiglia, and P. J. McKeown-Longo Anastellin, a Fragment of the First Type III Repeat of Fibronectin, Inhibits Extracellular Signal-Regulated Kinase and Causes G1 Arrest in Human Microvessel Endothelial Cells Cancer Res., January 1, 2005; 65(1): 148 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Pompe, L. Renner, and C. Werner Nanoscale Features of Fibronectin Fibrillogenesis Depend on Protein-Substrate Interaction and Cytoskeleton Structure Biophys. J., January 1, 2005; 88(1): 527 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Ingham, S. Brew, D. Vaz, D. N. Sauder, and M. J. McGavin Interaction of Staphylococcus aureus Fibronectin-binding Protein with Fibronectin: AFFINITY, STOICHIOMETRY, AND MODULAR REQUIREMENTS J. Biol. Chem., October 8, 2004; 279(41): 42945 - 42953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Robinson, R. A. Foty, and S. A. Corbett Fibronectin Matrix Assembly Regulates {alpha}5{beta}1-mediated Cell Cohesion Mol. Biol. Cell, March 1, 2004; 15(3): 973 - 981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Monaghan, V. Gueorguiev, C. Wilkins-Port, and P. J. McKeown-Longo The Receptor for Urokinase-type Plasminogen Activator Regulates Fibronectin Matrix Assembly in Human Skin Fibroblasts J. Biol. Chem., January 9, 2004; 279(2): 1400 - 1407. [Abstract] [Full Text] [PDF]
|
|
