spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 2 July 2003
doi: 10.1242/jcs.00639


This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00639v1
116/16/3295    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Rogister, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wislet-Gendebien, S.
Right arrow Articles by Rogister, B.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Cell Science 116, 3295-3302 (2003)
doi: 10.1242/jcs.00639


Research Article

Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells

S. Wislet-Gendebien1,*, P. Leprince1, G. Moonen1 and B. Rogister1,2

1 Center for Cellular and Molecular Neurobiology, University of Liège, 17 Place Delcour, B-4020 Liège, Belgium
2 Department of Neurology, University of Liège, C.H.U. (B35) Sart Tilman, B-4000 Liège, Belgium

* Author for correspondence (e-mail: s.wislet{at}ulg.ac.be)

Accepted 28 April 2003

Bone marrow stromal cells can differentiate into many types of mesenchymal cells, i.e. osteocyte, chondrocyte and adipocyte, but can also differentiate into non-mesenchymal cells, i.e. neural cells under appropriate in vivo experimental conditions (Kopen et al., 1999; Brazelton et al., 2000; Mezey et al., 2000). This neural phenotypic plasticity allows us to consider the utilization of mesenchymal stem cells as cellular material in regenerative medicine. In this study, we demonstrate that cultured adult rat stromal cells can express nestin, an intermediate filament protein predominantly expressed by neural stem cells. Two factors contribute to the regulation of nestin expression by rat stromal cells: serum in the culture medium inhibits nestin expression and a threshold number of passages must be reached below which nestin expression does not occur. Only nestin-positive rat stromal cells are able to form spheres when they are placed in the culture conditions used for neural stem cells. Likewise, only nestin-positive stromal cells are able to differentiate into GFAP (glial fibrillary acidic protein)-positive cells when they are co-cultivated with neural stem cells. We thus demonstrated that adult rat stromal cells in culture express nestin in absence of serum after passaging the cells at least ten times, and we suggest that nestin expression by these cells might be a prerequisite for the acquisition of the capacity to progress towards the neural lineage.

Key words: Nestin, Bone marrow stromal cells, GFAP, Neural stem cells, Glial differentiation


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
Stem CellsHome page
L. Lu, X. Chen, C.-W. Zhang, W.-L. Yang, Y.-J. Wu, L. Sun, L.-M. Bai, X.-S. Gu, S. Ahmed, G. S. Dawe, et al.
Morphological and Functional Characterization of Predifferentiation of Myelinating Glia-Like Cells from Human Bone Marrow Stromal Cells Through Activation of F3/Notch Signaling in Mouse Retina
Stem Cells, February 1, 2008; 26(2): 580 - 590.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. G. Phinney and D. J. Prockop
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair Current Views
Stem Cells, November 1, 2007; 25(11): 2896 - 2902.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
F. Anjos-Afonso and D. Bonnet
Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment
Blood, February 1, 2007; 109(3): 1298 - 1306.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
P. Tropel, N. Platet, J.-C. Platel, D. Noel, M. Albrieux, A.-L. Benabid, and F. Berger
Functional Neuronal Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells, December 1, 2006; 24(12): 2868 - 2876.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
C. Wiese, A. Rolletschek, G. Kania, A. Navarrete-Santos, S. V. Anisimov, B. Steinfarz, K. V. Tarasov, S. A. Brugh, I. Zahanich, C. Ruschenschmidt, et al.
Signals from Embryonic Fibroblasts Induce Adult Intestinal Epithelial Cells to Form Nestin-Positive Cells with Proliferation and Multilineage Differentiation Capacity In Vitro
Stem Cells, September 1, 2006; 24(9): 2085 - 2097.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
A. P. Croft and S. A. Przyborski
Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture.
Stem Cells, August 1, 2006; 24(8): 1841 - 1851.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
E. Pacary, H. Legros, S. Valable, P. Duchatelle, M. Lecocq, E. Petit, O. Nicole, and M. Bernaudin
Synergistic effects of CoCl2 and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells
J. Cell Sci., July 1, 2006; 119(13): 2667 - 2678.
[Abstract] [Full Text] [PDF]


Home page
HypertensionHome page
V. El-Helou, J. Dupuis, C. Proulx, J. Drapeau, R. Clement, H. Gosselin, L. Villeneuve, L. Manganas, and A. Calderone
Resident Nestin+ Neural-Like Cells and Fibers Are Detected in Normal and Damaged Rat Myocardium
Hypertension, November 1, 2005; 46(5): 1219 - 1225.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
N. Chen, J. E. Hudson, P. Walczak, I. Misiuta, S. Garbuzova-Davis, L. Jiang, J. Sanchez-Ramos, P. R. Sanberg, T. Zigova, and A. E. Willing
Human Umbilical Cord Blood Progenitors: The Potential of These Hematopoietic Cells to Become Neural
Stem Cells, October 1, 2005; 23(10): 1560 - 1570.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
N. Bertani, P. Malatesta, G. Volpi, P. Sonego, and R. Perris
Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray
J. Cell Sci., September 1, 2005; 118(17): 3925 - 3936.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
S. Wislet-Gendebien, G. Hans, P. Leprince, J.-M. Rigo, G. Moonen, and B. Rogister
Plasticity of Cultured Mesenchymal Stem Cells: Switch from Nestin-Positive to Excitable Neuron-Like Phenotype
Stem Cells, March 1, 2005; 23(3): 392 - 402.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2003