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First published online 26 June 2003
doi: 10.1242/jcs.00631

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Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1

¹ Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA
² Departments of Paediatrics and Physiology and Pharmacology, Child Health Research Institute, University of Western Ontario, London, Ontario N6C 2V5, Canada

^* Author for correspondence (e-mail: sfk{at}bilbo.bio.purdue.edu)

Accepted 23 April 2003

Gap junctions are intercellular channels that provide direct passage of small molecules between adjacent cells. In pancreatic acini, the connexin26 (Cx26) and connexin32 (Cx32) proteins form functional channels that coordinate the secretion of digestive enzymes. Although the function of Cx26/Cx32 gap junctions are well characterized, the regulatory circuits that control the spatial and temporal expression patterns of these connexin genes are not known. In an effort to identify the molecular pathways that regulate connexin gene expression, we examined Cx26 and Cx32 gene activities in mice lacking the basic helix-loop-helix transcription factor Mist1 (Mist1^KO). Mist1, Cx26 and Cx32 are co-expressed in most exocrine cell types, and acinar cells from Mist1^KO mice exhibit a highly disorganized cellular architecture and an altered pattern of expression for several genes involved in regulated exocytosis. Analysis of Mist1^KO mice revealed a dramatic decrease in both connexin proteins, albeit through different molecular mechanisms. Cx32 gene transcription was greatly reduced in all Mist1^KO exocrine cells, while Cx26 gene expression remained unaffected. However, in the absence of Cx32 protein, Cx26 did not participate in gap junction formation, leading to a complete lack of intercellular communication among Mist1^KO acinar cells. Additional studies testing Mist1 gene constructs in pancreatic exocrine cells confirmed that Mist1 transcriptionally regulates expression of the Cx32 gene. We conclude that Mist1 functions as a positive regulator of Cx32 gene expression and, in its absence, acinar cell gap junctions and intercellular communication pathways become disrupted.

Key words: Pancreas, Exocytosis, bHLH, Transcription, Cell communication

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