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First published online 2 July 2003
doi: 10.1242/jcs.00634
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Research Article |
1 Department of Molecular Biology and Biotechnology, University of Sheffield,
Sheffield S10 2TN, UK
2 Cardiff School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10
3TL, UK
* Author for correspondence (e-mail: P.Sudbery{at}shef.ac.uk)
Accepted 25 April 2003
Isoamyl alcohol (IAA) induces a phenotype that resembles pseudohyphae in
the budding yeast Saccharomyces cerevisiae. We show here that IAA
causes the rapid formation of linear chains of anucleate buds, each of which
is accompanied by the formation of a septin ring at its neck. This process
requires the activity of Swe1 and Slt2 (Mpk1). Cdc28 is phosphorylated on
tyrosine 19 in a Swe1-dependent manner, while Slt2 becomes activated by dual
tyrosine/threonine phosphorylation. Tyrosine 19 phosphorylation of Cdc28 is
not dependent on Slt2. However, the defective response in the
slt2
mutant is rescued by an mih1 mutation.
The IAA response still occurs in a cell containing a dominant
non-phosphorylatable form of Cdc28, but no longer occurs in an
mih1 slt2 mutant containing this form of
Cdc28. These observations show that IAA induces the Swe1-dependent
morphogenesis checkpoint and so the resulting pseudohyphal phenotype arises in
an entirely different way from the formation of pseudohyphae induced by
nitrogen-limited growth.
Key words: Morphogenesis checkpoint, Isoamyl alcohol, Pseudohyphae, SLT2, SWE1, Yeast
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