|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 22 July 2003
doi: 10.1242/jcs.00678
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Ruhr-Universität Bochum, Institut für Physiologische Chemie,
Medizinische Fakultät, 44780 Bochum, Germany
2 Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe,
Postfach 3640, 76021 Karlsruhe, Germany
* Author for correspondence (e-mail: nils.johnsson{at}itg.fzk.de)
Accepted 21 May 2003
The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.
Key words: Membrane proteins, Protein interaction, Peroxins, Peroxisome, Protein import, Split-ubiquitin
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Rahim, S. Bischof, F. Kessler, and B. Agne In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system J. Exp. Bot., January 1, 2009; 60(1): 257 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Grou, A. F. Carvalho, M. P. Pinto, S. Wiese, H. Piechura, H. E. Meyer, B. Warscheid, C. Sa-Miranda, and J. E. Azevedo Members of the E2D (UbcH5) Family Mediate the Ubiquitination of the Conserved Cysteine of Pex5p, the Peroxisomal Import Receptor J. Biol. Chem., May 23, 2008; 283(21): 14190 - 14197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Apraiz, J. Mi, and S. Cristobal Identification of Proteomic Signatures of Exposure to Marine Pollutants in Mussels (Mytilus edulis) Mol. Cell. Proteomics, July 1, 2006; 5(7): 1274 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kohlstedt, C. Gershome, M. Friedrich, W. Muller-Esterl, F. Alhenc-Gelas, R. Busse, and I. Fleming Angiotensin-Converting Enzyme (ACE) Dimerization Is the Initial Step in the ACE Inhibitor-Induced ACE Signaling Cascade in Endothelial Cells Mol. Pharmacol., May 1, 2006; 69(5): 1725 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Zolman, M. Monroe-Augustus, I. D. Silva, and B. Bartel Identification and Functional Characterization of Arabidopsis PEROXIN4 and the Interacting Protein PEROXIN22 PLANT CELL, December 1, 2005; 17(12): 3422 - 3435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fan, S. Quan, T. Orth, C. Awai, J. Chory, and J. Hu The Arabidopsis PEX12 Gene Is Required for Peroxisome Biogenesis and Is Essential for Development Plant Physiology, September 1, 2005; 139(1): 231 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chavan, A. Yan, and W. J. Lennarz Subunits of the Translocon Interact with Components of the Oligosaccharyl Transferase Complex J. Biol. Chem., June 17, 2005; 280(24): 22917 - 22924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel The Saccharomyces cerevisiae Peroxisomal Import Receptor Pex5p Is Monoubiquitinated in Wild Type Cells J. Biol. Chem., March 4, 2005; 280(9): 7867 - 7874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shiozawa, N. Maita, K. Tomii, A. Seto, N. Goda, Y. Akiyama, T. Shimizu, M. Shirakawa, and H. Hiroaki Structure of the N-terminal Domain of PEX1 AAA-ATPase: CHARACTERIZATION OF A PUTATIVE ADAPTOR-BINDING DOMAIN J. Biol. Chem., November 26, 2004; 279(48): 50060 - 50068. [Abstract] [Full Text] [PDF]
|
|
