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First published online 22 July 2003
doi: 10.1242/jcs.00678
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Research Article |
1 Ruhr-Universität Bochum, Institut für Physiologische Chemie,
Medizinische Fakultät, 44780 Bochum, Germany
2 Institut für Toxikologie und Genetik, Forschungszentrum Karlsruhe,
Postfach 3640, 76021 Karlsruhe, Germany
* Author for correspondence (e-mail: nils.johnsson{at}itg.fzk.de)
Accepted 21 May 2003
The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.
Key words: Membrane proteins, Protein interaction, Peroxins, Peroxisome, Protein import, Split-ubiquitin
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