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First published online October 22, 2003
doi: 10.1242/10.1242/jcs.00748
Research Article |
1 Laboratory of Cellular Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
2 Department of Biology, Chemistry, Pharmacology, Free University Berlin, 14195 Berlin, Germany
3 Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10021, USA
* Author for correspondence (e-mail: simon{at}mail.rockefeller.edu)
Accepted 7 July 2003
Cell migration might involve biased membrane traffic toward the leading edge to facilitate the building of extracellular matrix, membrane protrusions and adhesion plaques. We tested the hypothesis that secretory vesicles are preferentially delivered toward the leading lamella in wound-edge fibroblasts. Single fusion events of vesicles containing LDLR-GFP were mapped by total internal reflection fluorescence microscopy (TIR-FM). In migrating fibroblasts, exocytic events were polarized towards the leading edge. After disrupting microtubules with nocodazole, exocytosis continued, but fusion sites were clustered around central Golgi elements; there was no peripheral exocytosis. We conclude that microtubules are necessary for the domain-specific fusion of post-Golgi vesicles with the plasma membrane during migration.
Key words: Total internal reflection, Evanescent wave, Cell polarization, Post-Golgi vesicle, Migration, Secretion
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