QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 11 December 2002
doi: 10.1242/jcs.00250

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.00250v1 116/3/487    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weingartner, M. Articles by Bögre, L. Search for Related Content PubMed PubMed Citation Articles by Weingartner, M. Articles by Bögre, L. Social Bookmarking                         What's this?

A plant cyclin B2 is degraded early in mitosis and its ectopic expression shortens G2-phase and alleviates the DNA-damage checkpoint

¹ Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, Dr Bohrgasse 9, A-1030 Vienna, Austria
² Centro de Investigaciones Biológicas CSIC, Velázquez 144, E-28006-Madrid, Spain
³ Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeská 1083, 142 20 Prague 4, Czech Republic
⁴ School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, UK

^* Author for correspondence (e-mail: l.bogre{at}rhul.ac.uk)

Accepted 31 October 2002

Mitotic progression is timely regulated by the accumulation and degradation of A- and B-type cyclins. In plants, there are three classes of A-, and two classes of B-type cyclins, but their specific roles are not known. We have generated transgenic tobacco plants in which the ectopic expression of a plant cyclin B2 gene is under the control of a tetracycline-inducible promoter. We show that the induction of cyclin B2 expression in cultured cells during G2 phase accelerates the entry into mitosis and allows cells to override the replication checkpoint induced by hydroxyurea in the simultaneous presence of caffeine or okadaic acid, drugs that are known to alleviate checkpoint control. These results indicate that in plants, a B2-type cyclin is a rate-limiting regulator for the entry into mitosis and a cyclin B2-CDK complex might be a target for checkpoint control pathways. The cyclin B2 localization and the timing of its degradation during mitosis corroborate these conclusions: cyclin B2 protein is confined to the nucleus and during mitosis it is only present during a short time window until mid prophase, but it is effectively degraded from this timepoint onwards. Although cyclin B2 is not present in cells arrested by the spindle checkpoint in metaphase, cyclin B1 is accumulating in these cells. Ectopic expression of cyclin B2 in developing plants interferes with differentiation events and specifically blocks root regeneration, indicating the importance of control mechanisms at the G2- to M-phase transition during plant developmental processes.

Key words: Cyclin B, Mitosis, Checkpoint, Plant development

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. T. Polit and A. Kazmierczak Okadaic acid (1 {micro}M) accelerates S phase and mitosis but inhibits heterochromatin replication and metaphase-anaphase transition in Vicia faba meristem cells J. Exp. Bot., August 1, 2007; 58(11): 2785 - 2797. [Abstract] [Full Text] [PDF]

				K. De Schutter, J. Joubes, T. Cools, A. Verkest, F. Corellou, E. Babiychuk, E. Van Der Schueren, T. Beeckman, S. Kushnir, D. Inze, et al. Arabidopsis WEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint PLANT CELL, January 1, 2007; 19(1): 211 - 225. [Abstract] [Full Text] [PDF]

				S. Adachi, H. Uchimiya, and M. Umeda Expression of B2-Type Cyclin-Dependent Kinase is Controlled by Protein Degradation in Arabidopsis thaliana Plant Cell Physiol., December 1, 2006; 47(12): 1683 - 1686. [Abstract] [Full Text] [PDF]

				N. Correa-Aragunde, M. Graziano, C. Chevalier, and L. Lamattina Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato J. Exp. Bot., February 1, 2006; 57(3): 581 - 588. [Abstract] [Full Text] [PDF]

				Z. Magyar, L. De Veylder, A. Atanassova, L. Bako, D. Inze, and L. Bogre The Role of the Arabidopsis E2FB Transcription Factor in Regulating Auxin-Dependent Cell Division PLANT CELL, September 1, 2005; 17(9): 2527 - 2541. [Abstract] [Full Text] [PDF]

				M. Umeda, A. Shimotohno, and M. Yamaguchi Control of Cell Division and Transcription by Cyclin-dependent Kinase-activating Kinases in Plants Plant Cell Physiol., September 1, 2005; 46(9): 1437 - 1442. [Abstract] [Full Text] [PDF]

				M. Weingartner, M.-C. Criqui, T. Meszaros, P. Binarova, A.-C. Schmit, A. Helfer, A. Derevier, M. Erhardt, L. Bogre, and P. Genschik Expression of a Nondegradable Cyclin B1 Affects Plant Development and Leads to Endomitosis by Inhibiting the Formation of a Phragmoplast PLANT CELL, March 1, 2004; 16(3): 643 - 657. [Abstract] [Full Text] [PDF]

				T. Garcia-Muse, G. Steinberg, and J. Perez-Martin Characterization of B-type cyclins in the smut fungus Ustilago maydis: roles in morphogenesis and pathogenicity J. Cell Sci., January 22, 2004; 117(3): 487 - 506. [Abstract] [Full Text] [PDF]

				Y. Yu, A. Steinmetz, D. Meyer, S. Brown, and W.-H. Shen The Tobacco A-Type Cyclin, Nicta;CYCA3;2, at the Nexus of Cell Division and Differentiation PLANT CELL, December 1, 2003; 15(12): 2763 - 2777. [Abstract] [Full Text] [PDF]

				M. Yamaguchi, H. Kato, S. Yoshida, S. Yamamura, H. Uchimiya, and M. Umeda Control of in vitro organogenesis by cyclin-dependent kinase activities in plants PNAS, June 24, 2003; 100(13): 8019 - 8023. [Abstract] [Full Text] [PDF]