spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 8 January 2003
doi: 10.1242/jcs.00230

This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00230v1
116/5/785    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vonna, L.
Right arrow Articles by Sackmann, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vonna, L.
Right arrow Articles by Sackmann, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Cell Science 116, 785-790 (2003)
doi: 10.1242/jcs.00230

Research Article

Local force induced conical protrusions of phagocytic cells

Laurent Vonna1,*, Agnès Wiedemann2, Martin Aepfelbacher2 and Erich Sackmann1,{ddagger}

1 Physik Depatment E22 (biophysics group), Technische Universität München, D-85748 Garching, Germany
2 Max von Pettenkofer Institut für Medizinische Mikrobiologie, Pettenkoferstrasse 9, Ludwig Maximilian Universität, D-80336 München, Germany
{ddagger} Present address: Institute of Theoretical Physics, UCSB, Santa Barbara, USA

* Author for correspondence (e-mail: lvonna{at}ph.tum.de)

Accepted 18 October 2002

Magnetic tweezers were used to study the passive and active response of macrophages to local centripetal nanonewton forces on ß1 integrins. Superparamagnetic beads coated with the ß1-integrin-binding protein invasin were attached to J774 murine macrophages to mimic phagocytosis of bacterial pathogens. Forces exceeding ~0.5 nN induce the active formation of trumpet-like protrusions resembling pseudopodia after an initial elastic deflection and a response time of ~30 seconds. The speed of advancement of the protrusion is <v>=0.065±0.020 µm second-1 and is force independent. After saturation (after about 100 seconds) the protrusion stops abruptly and is completely retracted again against forces exceeding 5 nN with an effective relaxation time of ~30 seconds. The active protrusion is tentatively attributed to the growth of the actin cortex in the direction of the force, and evidence for the involvement of actin is provided by the finding that Latrunculin A abolishes the activated cone growth. The growth is assumed to be activated by cell signaling mediated by the invasin-specific integrins (exhibiting ß1 chains) and could play a role in phagocytic and protrusive events during immune response by macrophages.

Key words: Magnetic tweezers, Phagocytosis, Yersinia, Invasin, Macrophage, J774, Protrusion


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. Herant, V. Heinrich, and M. Dembo
Mechanics of neutrophil phagocytosis: experiments and quantitative models.
J. Cell Sci., May 1, 2006; 119(Pt 9): 1903 - 1913.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003