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First published online 23 January 2003
doi: 10.1242/jcs.00282

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.00282v1 116/5/919    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kubo, A. Articles by Tsukita, S. Search for Related Content PubMed PubMed Citation Articles by Kubo, A. Articles by Tsukita, S.

Non-membranous granular organelle consisting of PCM-1: subcellular distribution and cell-cycle-dependent assembly/disassembly

¹ Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan
² Solution Oriented Research for Science and Technology, Japan Science and Technology Corporation, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan

* Author for correspondence (e-mail: htsukita{at}mfour.med.kyoto-u.ac.jp)

Accepted 19 November 2002

Centriolar satellites were initially identified as electrondense spherical granules, 70-100 nm in diameter, localized around the centrosomes. We have previously identified pericentriolar material 1 (PCM-1), with a molecular mass of 230 kDa, as a component of centriolar satellites. We now show by immunofluorescence microscopy that these granules are not only concentrated around centrioles but also scattered throughout the cytoplasm in various types of mouse cells, leading us tentatively to call them `PCM-1 granules'. We then found that, when overexpressed, PCM-1 molecules lacking their C-terminal region bound directly with each other through two distinct regions to form large aggregates, which then recruited endogenous PCM-1. These large aggregates as well as endogenous PCM-1 granules appear to be disassembled during mitosis, and reassembled when the cells entered interphase. These findings suggest that PCM-1 granules are formed by self-aggregation of PCM-1 and that this self-aggregation is regulated in a cell-cycle-dependent manner. Furthermore, we found that PCM-1 granules are distinct from pericentrin-containing granules, and that these two distinct types of granular structures are frequently associated with each other within the cytoplasm. These findings are discussed with special reference to the possible physiological functions of PCM-1 granules.

Key words: Centriolar satellites, PCM-1, Centrosome, Pericentrin, -Tubulin

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