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First published online 12 February 2003
doi: 10.1242/jcs.00317

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Myosin Va facilitates the distribution of secretory granules in the F-actin rich cortex of PC12 cells

Rüdiger Rudolf¹, Tanja Kögel¹, Sergei A. Kuznetsov^1,*, Thorsten Salm¹, Oliver Schlicker¹, Andrea Hellwig¹, John A. Hammer, III² and Hans-Hermann Gerdes^1,

¹ Department of Neurobiology, Interdisciplinary Center of Neuroscience, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany
² Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
^* Present address: Institute of Cell Biology and Biosystems Technology, University of Rostock, Albert-Einstein Str. 3, D-18051 Rostock, Germany

Author for correspondence (e-mail: hhgerdes{at}uni-hd.de)

Accepted 12 December 2002

Neuroendocrine secretory granules, the storage organelles for neuropeptides and hormones, are formed at the trans-Golgi network, stored inside the cell and exocytosed upon stimulation. Previously, we have reported that newly formed secretory granules of PC12 cells are transported in a microtubule-dependent manner from the trans-Golgi network to the F-actin-rich cell cortex, where they undergo short directed movements and exhibit a homogeneous distribution. Here we provide morphological and biochemical evidence that myosin Va is associated with secretory granules. Expression of a dominant-negative tail domain of myosin Va in PC12 cells led to an extensive clustering of secretory granules close to the cell periphery, a loss of their cortical restriction and a strong reduction in their motility in the actin cortex. Based on this data we propose a model that implies a dual transport system for secretory granules: after microtubule-dependent delivery to the cell periphery, secretory granules exhibit a myosin Va-dependent transport leading to their restriction and even dispersal in the F-actin-rich cortex of PC12 cells.

Key words: Secretory granules, Myosin Va, hCgB-GFP, Cell cortex, F-actin, Organelle transport

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