|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 18 March 2003
doi: 10.1242/jcs.00369
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and
Veterinary Science, Adelaide, South Australia, Australia
2 Myeloma and Mesenchymal Research Group, Matthew Robert's Foundation
laboratory, Institute of Medical and Veterinary Science, Adelaide, South
Australia, Australia
3 Department of Orthopaedics and Trauma, Adelaide University, Adelaide, South
Australia, Australia
4 Craniofacial and Skeletal Diseases Branch, National Institute of Dental &
Craniofacial Research, National Institutes of Health, Maryland, USA
5 Department of Orthopaedics, Royal Melbourne Hospital, Melbourne, Victoria,
Australia
6 Stem Cell Laboratory, Peter MacCallum Cancer Institute, East Melbourne,
Victoria, Australia
* Author for correspondence (e-mail: stan.gronthos{at}imvs.sa.gov.au)
Accepted 14 January 2003
Previous studies have provided evidence for the existence of adult human bone marrow stromal stem cells (BMSSCs) or mesenchymal stem cells. Using a combination of cell separation techniques, we have isolated an almost homogeneous population of BMSSCs from adult human bone marrow. Lacking phenotypic characteristics of leukocytes and mature stromal elements, BMSSCs are non-cycling and constitutively express telomerase activity in vivo. This mesenchymal stem cell population demonstrates extensive proliferation and retains the capacity for differentiation into bone, cartilage and adipose tissue in vitro. In addition, clonal analysis demonstrated that individual BMSSC colonies exhibit a differential capacity to form new bone in vivo. These data are consistent with the existence of a second population of bone marrow stem cells in addition to those for the hematopoietic system. Our novel selection protocol provides a means to generate purified populations of BMSSCs for use in a range of different tissue engineering and gene therapy strategies.
Key words: Bone Marrow Stroma, Mesenchymal Stem Cells, STRO-1, Bone, Cartilage, Adipose, CFU-F
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  G.T.-J. Huang, S. Gronthos, and S. Shi Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine Journal of Dental Research, September 1, 2009; 88(9): 792 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Arthur, S. Koblar, S. Shi, and S. Gronthos Eph/ephrinB Mediate Dental Pulp Stem Cell Mobilization and Function Journal of Dental Research, September 1, 2009; 88(9): 829 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Gargett, K. E. Schwab, R. M. Zillwood, H. P.T. Nguyen, and D. Wu Isolation and Culture of Epithelial Progenitors and Mesenchymal Stem Cells from Human Endometrium Biol Reprod, June 1, 2009; 80(6): 1136 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zheng, Y. Liu, C.M. Zhang, H.Y. Zhang, W.H. Li, S. Shi, A.D. Le, and S.L. Wang Stem Cells from Deciduous Tooth Repair Mandibular Defect in Swine Journal of Dental Research, March 1, 2009; 88(3): 249 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Toma, W. R. Wagner, S. Bowry, A. Schwartz, and F. Villanueva Fate Of Culture-Expanded Mesenchymal Stem Cells in The Microvasculature: In Vivo Observations of Cell Kinetics Circ. Res., February 13, 2009; 104(3): 398 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Lee, M. J. Seo, A. A. Pulin, C. A. Gregory, J. Ylostalo, and D. J. Prockop The CD34-like protein PODXL and {alpha}6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice Blood, January 22, 2009; 113(4): 816 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Krause Bone Marrow-derived Cells and Stem Cells in Lung Repair Proceedings of the ATS, April 15, 2008; 5(3): 323 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.E. Schwab, P. Hutchinson, and C.E. Gargett Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells Hum. Reprod., April 1, 2008; 23(4): 934 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Delorme, J. Ringe, N. Gallay, Y. Le Vern, D. Kerboeuf, C. Jorgensen, P. Rosset, L. Sensebe, P. Layrolle, T. Haupl, et al. Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells Blood, March 1, 2008; 111(5): 2631 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Jones and D. McGonagle Human bone marrow mesenchymal stem cells in vivo Rheumatology, February 1, 2008; 47(2): 126 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C.W. Zannettino, S. Paton, A. Kortesidis, F. Khor, S. Itescu, and S. Gronthos Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1bright/CD34 /CD45 /glycophorin-A-bone marrow cells Haematologica, December 1, 2007; 92(12): 1707 - 1708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Nauta and W. E. Fibbe Immunomodulatory properties of mesenchymal stromal cells Blood, November 15, 2007; 110(10): 3499 - 3506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rubinek, V. Chesnokova, I. Wolf, K. Wawrowsky, G. Vlotides, and S. Melmed Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1082 - C1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lue, K. Erkkila, P. Y. Liu, K. Ma, C. Wang, A. S. Hikim, and R. S. Swerdloff Fate of Bone Marrow Stem Cells Transplanted into the Testis: Potential Implication for Men with Testicular Failure Am. J. Pathol., March 1, 2007; 170(3): 899 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Anjos-Afonso and D. Bonnet Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment Blood, February 1, 2007; 109(3): 1298 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.E. Gargett Uterine stem cells: What is the evidence? Hum. Reprod. Update, January 1, 2007; 13(1): 87 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.J. Mao, W.V. Giannobile, J.A. Helms, S.J. Hollister, P.H. Krebsbach, M.T. Longaker, and S. Shi Craniofacial Tissue Engineering by Stem Cells Journal of Dental Research, November 1, 2006; 85(11): 966 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Noer, A. L. Sorensen, A. C. Boquest, and P. Collas Stable CpG Hypomethylation of Adipogenic Promoters in Freshly Isolated, Cultured, and Differentiated Mesenchymal Stem Cells from Adipose Tissue Mol. Biol. Cell, August 1, 2006; 17(8): 3543 - 3556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Munoz-Fernandez, F. J. Blanco, C. Frecha, F. Martin, M. Kimatrai, A. C. Abadia-Molina, J. M. Garcia-Pacheco, and E. G. Olivares Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts J. Immunol., July 1, 2006; 177(1): 280 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Simmons, B. Short, and N. Brouard The Properties of Prospectively Isolated Mesenchymal Stem Cells from Man and Mouse Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 334 - 338. [Full Text] [PDF]
|
|
|
|
|
|
D. Baksh, J. E. Davies, and P. W. Zandstra Soluble factor cross-talk between human bone marrow-derived hematopoietic and mesenchymal cells enhances in vitro CFU-F and CFU-O growth and reveals heterogeneity in the mesenchymal progenitor cell compartment Blood, November 1, 2005; 106(9): 3012 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Miura, M. Miura, S. Gronthos, M. R. Allen, C. Cao, T. E. Uveges, Y. Bi, D. Ehirchiou, A. Kortesidis, S. Shi, et al. Defective osteogenesis of the stromal stem cells predisposes CD18-null mice to osteoporosis PNAS, September 27, 2005; 102(39): 14022 - 14027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kortesidis, A. Zannettino, S. Isenmann, S. Shi, T. Lapidot, and S. Gronthos Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cells Blood, May 15, 2005; 105(10): 3793 - 3801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Boquest, A. Shahdadfar, K. Fronsdal, O. Sigurjonsson, S. H. Tunheim, P. Collas, and J. E. Brinchmann Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem Cells: Alteration of Gene Expression after In Vitro Cell Culture Mol. Biol. Cell, March 1, 2005; 16(3): 1131 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Thomas, S. A. Johnson, N. A. Sims, M. K. Trivett, J. L. Slavin, B. P. Rubin, P. Waring, G. A. McArthur, C. R. Walkley, A. J. Holloway, et al. Terminal osteoblast differentiation, mediated by runx2 and p27KIP1, is disrupted in osteosarcoma J. Cell Biol., December 6, 2004; 167(5): 925 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakaguchi, I. Sekiya, K. Yagishita, S. Ichinose, K. Shinomiya, and T. Muneta Suspended cells from trabecular bone by collagenase digestion become virtually identical to mesenchymal stem cells obtained from marrow aspirates Blood, November 1, 2004; 104(9): 2728 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Pittenger and B. J. Martin Mesenchymal Stem Cells and Their Potential as Cardiac Therapeutics Circ. Res., July 9, 2004; 95(1): 9 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D'Ippolito, S. Diabira, G. A. Howard, P. Menei, B. A. Roos, and P. C. Schiller Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential J. Cell Sci., June 15, 2004; 117(14): 2971 - 2981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Herzog, L. Chai, and D. S. Krause Plasticity of marrow-derived stem cells Blood, November 15, 2003; 102(10): 3483 - 3493. [Abstract] [Full Text] [PDF]
|
|
