spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 19 November 2003
doi: 10.1242/jcs.00832

Journal of Cell Science 117, 63-72 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00832v1
117/1/63    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quittau-Prévostel, C.
Right arrow Articles by Joubert, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quittau-Prévostel, C.
Right arrow Articles by Joubert, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Targeting of PKC{alpha} and {epsilon} in the pituitary: a highly regulated mechanism involving a GD(E)E motif of the V3 region

Corinne Quittau-Prévostel, Nathalie Delaunay, Alejandra Collazos, Alice Vallentin* and Dominique Joubert{ddagger}

Inserm U469 `Molecular and Cellular Endocrinology: Signaling and Pathology', 141 rue de la Cardonille, 34094 Montpellier CEDEX 05, France

{ddagger} Author for correspondence (e-mail: dominique.joubert{at}ccipe.cnrs.fr)

Accepted 13 August 2003

Protein kinase C (PKC) has been implicated in the control of intercellular adhesion. Our previous observation demonstrating that activated PKC alpha (PKC{alpha} is selectively targeted to cell-cell contacts of pituitary GH3B6 cells supports these findings. The relevance of this observation is further strengthened by the present data establishing that this targeting selectivity also occurs in the pituitary gland. Moreover, a new mechanism involved in the control of PKC targeting is unravelled. We demonstrate that a three amino acid motif located in the V3 region of {alpha} and epsilon ({epsilon} (GDE/GEE respectively) is essential for the targeting selectivity of these isoforms because: (1) this motif is absent in delta ({delta}) and mutated in the natural D294GPKC{alpha} mutant, which do not exhibit such selectivity, and (2) a GEE to GGE mutation abolishes the selectivity of targeting to cell-cell contacts for {epsilon}, as it does for the D294G PKC{alpha} mutant. Thus the GD(E)E motif may be part of a consensus sequence able to interact with shuttle and/or anchoring proteins. GFP-tagged deletion mutants also reveal a new function for the pseudosubstrate in the cytoplasmic sequestration. Together, these data underline the complexity of PKC subcellular targeting in the pituitary, determined by the cell-cell contact, at least for {alpha} and {epsilon}

Key words: Protein kinase C, Targeting, Pituitary, Cell-cell contact


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Y. Jung, J.-M. Kim, H. K. Kang, D. H. Jang, and B.-M. Min
A Biologically Active Sequence of the Laminin {alpha}2 Large Globular 1 Domain Promotes Cell Adhesion through Syndecan-1 by Inducing Phosphorylation and Membrane Localization of Protein Kinase C{delta}
J. Biol. Chem., November 13, 2009; 284(46): 31764 - 31775.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Diouf, A. Collazos, G. Labesse, F. Macari, A. Choquet, P. Clair, C. Gauthier-Rouviere, N. C. Guerineau, P. Jay, F. Hollande, et al.
A 20-Amino Acid Module of Protein Kinase C{epsilon} Involved in Translocation and Selective Targeting at Cell-Cell Contacts
J. Biol. Chem., July 10, 2009; 284(28): 18808 - 18815.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C.-L. Chen and H.-C. Chen
Functional suppression of E-cadherin by protein kinase C{delta}
J. Cell Sci., February 15, 2009; 122(4): 513 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. F. Steinberg
Structural Basis of Protein Kinase C Isoform Function
Physiol Rev, October 1, 2008; 88(4): 1341 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C.-L. Chen, Y.-T. Hsieh, and H.-C. Chen
Phosphorylation of adducin by protein kinase C{delta} promotes cell motility
J. Cell Sci., April 1, 2007; 120(7): 1157 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Collazos, B. Diouf, N. C. Guerineau, C. Quittau-Prevostel, M. Peter, F. Coudane, F. Hollande, and D. Joubert
A Spatiotemporally Coordinated Cascade of Protein Kinase C Activation Controls Isoform-Selective Translocation.
Mol. Cell. Biol., March 1, 2006; 26(6): 2247 - 2261.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Louis, N. Guerineau, O. Fromigue, V. Defamie, A. Collazos, P. Anglard, M. A. Shipp, P. Auberger, D. Joubert, and B. Mari
Tumor Cell-mediated Induction of the Stromal Factor Stromelysin-3 Requires Heterotypic Cell Contact-dependent Activation of Specific Protein Kinase C Isoforms
J. Biol. Chem., January 14, 2005; 280(2): 1272 - 1283.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004