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First published online May 4, 2004
doi: 10.1242/10.1242/jcs.01041
Research Article |
1 Department of Internal Medicine, University of Lausanne, Lausanne, Switzerland
2 Institute of Cellular Biology and Morphology, University of Lausanne, Lausanne, Switzerland
* Author for correspondence (e-mail: gwaeber{at}chuv.hospvd.ch)
Accepted 4 December 2003
The neuronal-specific protein complexin I (CPX I) plays an important role in controlling the Ca2+-dependent neurotransmitter release. Since insulin exocytosis and neurotransmitter release rely on similar molecular mechanisms and that pancreatic ß-cells and neuronal cells share the expression of many restricted genes, we investigated the potential role of CPX I in insulin-secreting cells. We found that pancreatic islets and several insulin-secreting cell lines express high levels of CPX I. The ß-cell expression of CPX I is mediated by the presence of a neuron restrictive silencer element located within the regulatory region of the gene. This element bound the transcriptional repressor REST, which is found in most cell types with the exception of mature neuronal cells and ß-cells. Overexpression of CPX I or silencing of the CPX I gene (Cplx1) by RNA interference led to strong impairment in ß-cell secretion in response to nutrients such as glucose, leucine and KCl. This effect was detected both in the early and the sustained secretory phases but was much more pronounced in the early phase. We conclude that CPX I plays a critical role in ß-cells in the control of the stimulated-exocytosis of insulin.
Key words: Complexin I, Small interfering RNA, Secretion, Pancreatic ß-cells, Transcriptional repressor REST
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