|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online May 4, 2004
doi: 10.1242/10.1242/jcs.01092
Research Article |
1 Department of Biology, University of Leicester, University Road, Leicester, LE1 7RH, UK
2 Plant Functions Laboratory and Plant Science Center, RIKEN, Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Authors for correspondence (e-mail: mtf1{at}mac.com; sgm5{at}le.ac.uk)
Accepted 9 January 2004
Chloroplast division is mediated by the coordinated action of a prokaryote-derived division system(s) and a host eukaryote-derived membrane fission system(s). The evolutionary conserved prokaryote-derived system comprises several nucleus-encoded proteins, two of which are thought to control division site placement at the midpoint of the organelle: a stromal ATPase MinD and a topological specificity factor MinE. Here, we show that arc11, one of 12 recessive accumulation and replication of chloroplasts (arc) mutants in Arabidopsis, contains highly elongated and multiple-arrayed chloroplasts in developing green tissues. Genomic sequence analysis revealed that arc11 contains a missense mutation in 
-helix 11 of the chloroplast-targeted AtMinD1 changing an Ala at position 296 to Gly (A296G). Introduction of wild-type AtMinD1 restores the chloroplast division defects of arc11 and quantitative RT-PCR analysis showed that the degree of complementation was highly dependent on transgene expression levels. Overexpression of the mutant ARC11/AtMinD1 in transgenic plants results in the inhibition of chloroplast division, showing that the mutant protein has retained its division inhibition activity. However, in contrast to the defined and punctate intraplastidic localization patterns of an AtMinD1-YFP fusion protein, the single A296G point mutation in ARC11/AtMinD1 results in aberrant localization patterns inside chloroplasts. We further show that AtMinD1 is capable of forming homodimers and that this dimerization capacity is abolished by the A296G mutation in ARC11/AtMinD1. Our data show that arc11 is a loss-of-function mutant of AtMinD1 and suggest that the formation of functional AtMinD1 homodimers is paramount for appropriate AtMinD1 localization, ultimately ensuring correct division machinery placement and chloroplast division in plants.
Key words: Arabidopsis, Chloroplast division, Min system, arc mutant
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. T. Fujiwara, K. Sekine, Y. Y. Yamamoto, T. Abe, N. Sato, and R. D. Itoh Live Imaging of Chloroplast FtsZ1 Filaments, Rings, Spirals, and Motile Dot Structures in the AtMinE1 Mutant and Overexpressor of Arabidopsis thaliana Plant Cell Physiol., June 1, 2009; 50(6): 1116 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kazama, M. T. Fujiwara, A. Koizumi, K. Nishihara, R. Nishiyama, E. Kifune, T. Abe, and S. Kawano A SUPERMAN-like Gene is Exclusively Expressed in Female Flowers of the Dioecious Plant Silene latifolia Plant Cell Physiol., June 1, 2009; 50(6): 1127 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, T. Asano, M. T. Fujiwara, S. Yoshida, Y. Machida, and Y. Yoshioka Plant Cells Without Detectable Plastids are Generated in the crumpled leaf Mutant of Arabidopsis thaliana Plant Cell Physiol., May 1, 2009; 50(5): 956 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koniger, J. A. Delamaide, E. D. Marlow, and G. C. Harris Arabidopsis thaliana leaves with altered chloroplast numbers and chloroplast movement exhibit impaired adjustments to both low and high light J. Exp. Bot., June 1, 2008; 59(9): 2285 - 2297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Fujiwara, H. Hashimoto, Y. Kazama, T. Abe, S. Yoshida, N. Sato, and R. D. Itoh The Assembly of the FtsZ Ring at the Mid-Chloroplast Division Site Depends on a Balance Between the Activities of AtMinE1 and ARC11/AtMinD1 Plant Cell Physiol., March 1, 2008; 49(3): 345 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Maple and S. G. Moller Interdependency of formation and localisation of the Min complex controls symmetric plastid division J. Cell Sci., October 1, 2007; 120(19): 3446 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Maple and S. G. Moller Plastid Division: Evolution, Mechanism and Complexity Ann. Bot., April 1, 2007; 99(4): 565 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lopez-Juez Plastid biogenesis, between light and shadows J. Exp. Bot., January 1, 2007; 58(1): 11 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Forth and K. A. Pyke The suffulta mutation in tomato reveals a novel method of plastid replication during fruit ripening J. Exp. Bot., June 1, 2006; 57(9): 1971 - 1979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gao, T. L. Sage, and K. W. Osteryoung FZL, an FZO-like protein in plants, is a determinant of thylakoid and chloroplast morphology PNAS, April 25, 2006; 103(17): 6759 - 6764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Aldridge and S. G. Moller The Plastid Division Protein AtMinD1 Is a Ca2+-ATPase Stimulated by AtMinE1 J. Biol. Chem., September 9, 2005; 280(36): 31673 - 31678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Aldridge, J. Maple, and S. G. Moller The molecular biology of plastid division in higher plants J. Exp. Bot., April 1, 2005; 56(414): 1061 - 1077. [Abstract] [Full Text] [PDF]
|
|
