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First published online 5 May 2004
doi: 10.1242/jcs.01108
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Research Article |
1 Groupe Cycle Cellulaire – CNRS UMR6061 – IFR97, Génomique Fonctionnelle et Santé, Université de Rennes I, 2 avenue du Pr Léon Bernard, 35043 Rennes, France
2 LBCMCP-CNRS UMR5088-IFR109, Institut d'Exploration Fonctionnelle des Génomes, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
3 IPBS – CNRS UMR5089, 205 route de Narbonne, 31077 Toulouse, France
* Author for correspondence (e-mail: ducommun{at}cict.fr)
Accepted 19 January 2004
Aurora-A protein kinase, which is the product of an oncogene, is required for the assembly of a functional mitotic apparatus and the regulation of cell ploidy. Overexpression of Aurora-A in tumour cells has been correlated with cancer susceptibility and poor prognosis. Aurora-A activity is required for the recruitment of CDK1-cyclin B1 to the centrosome prior to its activation and the commitment of the cell to mitosis. In this report, we demonstrate that the CDC25B phosphatase, an activator of cyclin dependent kinases at mitosis, is phosphorylated both in vitro and in vivo by Aurora-A on serine 353 and that this phosphorylated form of CDC25B is located at the centrosome during mitosis. Knockdown experiments by RNAi confirm that the centrosome phosphorylation of CDC25B on S353 depends on Aurora-A kinase. Microinjection of antibodies against phosphorylated S353 results in a mitotic delay whilst overexpression of a S353 phosphomimetic mutant enhances the mitotic inducing effect of CDC25B. Our results demonstrate that Aurora-A phosphorylates CDC25B in vivo at the centrosome during mitosis. This phosphorylation might locally participate in the control of the onset of mitosis. These findings re-emphasise the role of the centrosome as a functional integrator of the pathways contributing to the triggering of mitosis.
Key words: Aurora-A, CDC25B phosphatase, Centrosome, Mitosis
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