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First published online 22 June 2004
doi: 10.1242/jcs.01162

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IL-4 alone without the involvement of GM-CSF transforms human peripheral blood monocytes to a CD1a^dim, CD83⁺ myeloid dendritic cell subset

Keshab Chandra Roy^*, Gautam Bandyopadhyay, Srabanti Rakshit, Mitali Ray and Santu Bandyopadhyay

The Division of Immunology, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Kolkata, 700 032, India

Author for correspondence (e-mail: santu2{at}iicb.res.in)

Accepted 16 February 2004

Myeloid dendritic cells (DCs) are conventionally generated by culturing human peripheral blood monocytes in the presence of GM-CSF and IL-4. Here we report that IL-4 alone, in the absence of detectable endogenous GM-CSF, transforms human peripheral blood monocytes to a CD1a^dim DC subset that could be matured to CD83⁺ DCs. Absence of endogenous GM-CSF in IL-4-DC was demonstrated by RT-PCR and flow cytometry. With the exception of CD1a expression, surface marker, morphology and phagocytic activity of these DCs (IL-4-DC) were similar to myeloid DCs (GM-IL-4-DC) conventionally generated in the presence of GM-CSF and IL-4. Conventional GM-IL-4-DC produced less IL-12 compared with IL-4-DC after stimulation with anti-CD40 monoclonal antibody, or LPS plus IFN-, although the difference was more prominent when LPS plus IFN- was used as the stimulus. The GM-IL-4-DC also induced less frequent IFN-⁺ T cells in a mixed leukocyte reaction (MLR) than that of IL-4-DC. Yields of IL-4-DCs were marginally lower than that of GM-IL-4-DCs. Our data indicate that peripheral blood monocytes can be transformed to CD1a-deficient myeloid DCs solely by IL-4, and these IL-4-DCs are likely to induce a stronger Th1 response than conventional GM-IL-4-DCs.

Key words: Dendritic cell, IL-4, Blood monocyte

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